Dr. Chris Carlton, LSU AgCenter Department of Entomology * Insect collection techniques. * Identification & classification of key rice pests

2. Rice Insects Taxonomy and Morphology: Personal Details of Some Old and New Foes Dr. Chris Carlton, Louisiana State Arthropod Museum LSU AgCenter, Dept of Entomology 9 to 9:45 am

4. Beetles (order Coleoptera): adults http://quasimodo.versailles. inra.fr/…/famtot.htm www.zin.ru/.../Coleoptera/ images/ M. Ferro www.zin.ru/.../Coleoptera/ images/ www.zin.ru/.../Coleoptera/ images/ www.zin.ru/.../Coleoptera/ images/

5. Beetles (order Coleoptera): adults Elytra completely covering flight wings Elytra partially exposing flight wings Thorax Abdomen Elytra Head Beetles of the World 1. Lawrence et al. 1999. Copyright CSIRO 1999.

6. Beetles (order Coleoptera): larvae Head Thorax Abdomen Head Thorax Abdomen

7. Rice water weevils: family Curculionidae http://www.padil.gov.au/pbt/files/uall/38RWW.jpg http://www.aragriculture.org/images/insects /rice/ricewaterweevillarva.jpg Adult Larva Lissorhoptrus oryzophilus Kuschel Lissorhoptrus simplex (Say) + 17 others in Central and South America

8. How to distinguish L. oryzophilus and L. simplex Have a look at this part of the hind leg (metatibia). L. oryzophilus L. simplex

9. What do rice water weevil larvae use to inflict feeding damage on rice? Like a combination of pointed, cutting, and gouging chisels. Mandibles Larval head, bottom view.

10. Rice water weevils cannot breath water, but live under it. How do they breath? Like a can opener + scuba gear, sorta. Larval spiracle used to access air pockets inside stem.

11. Rice levee billbug: family Curculionidae Sphenophorus spp. At least nine species occur in Louisiana. Species identification is important.

12. Colaspis leaf beetles: family Chrysomelidae Adult Larva

13. Their respective male sex organs (aedeagus). Colaspis louisianae Blake Colaspis brunnea (F.) Distinguishing Colaspis species

14. Colaspis feeding damage in Louisiana rice seems to mainly be caused by C. louisianae

15. And now for some good news, lady beetles: family Coccinellidae What people usually think of…

16. … but a lot of lady beetles are quite small and drab in appearance Diomus terminatus (Say)

17. … and are important as natural control agents of aphids. What is this Diomus larva doing to consume the aphid? Photo series, M. Ferro and W. Akbar Mandible

18. + + Piercing body wall, injecting digestive fluids, sucking down liquified contents… Like a combination of tongs, a syringe, and a shop vac.

19. True bugs (order Hemiptera)

20. Head Thorax Abdomen True bugs Beak Immatures similar to adults, only lacking wings.

21. Rice stink bug: family Pentatomidae Oebalus pugnax (F.)

22. Rice stink bug beak A simple straw?

23. Dissection reveals a more complex apparatus. The beak comprises six individual components. Labrum=upper lip Mand. stylets=catheter Max. stylets=straw Labium=sheath

24. Southern green stink bug: family Pentatomidae http://insects.tamu.edu/images/insects/color/sgstink1.jpg Nezara viridula (L.) Adult Immature (nymph) http://ipm.ncsu….Southern_green_stink_bug_nymph2.jpg Compare with: green stink bug, Acrosternum hilare (Say) http:// www.ent.iastate.edu/.../greenstinkbug.html

26. Identifying aphids requires microscope slidemounts of WINGED aphids

27. Detail showing sensory structures on antenna used in identification Slide mounted greenbug collected in 1969

28. Moths, butterflies, and skippers (order Lepidoptera ) www.images.on.ca/.../ Zebra_Swallowtail.jpg www.floridata.com/.../ cloudless_sulphur.jpg www.ybis.at/natur/ …Pa163070.jpg www.fcps.k12.va.us/.. /ppolblue.jpg www.leps.it/indexjs.htm?.../ThymeSylve.htm www.fcps.k12.va.us/. ../ppolblue.jpg

29. Lepidoptera characters http://static.howstuffworks.com/gif/butterfly-proboscis.jpg http:// www.greenville.k12.ny.us/.../cateranat.htm

30. Caterpiller feeding Like pinking shears. Mandibles Head, rear view.

32. http:// www.ipm.iastate.edu/.../armyworm.html http://mothphotographersgroup.msstate.edu/…Crambid%201a.jpg http:// commons.wikimedia.org/wiki/File:Adult_sugarca... Mexican ricer borer Rice stalk borer Sugarcane borer European corn borer

33. Comparing personal details of sugarcane borer and Mexican rice borer…male genitalia. Mexican ricer borer Sugarcane borer Slide mounted male genitalia of Mexican ricer borer 1 1 2 2 2 1 Reiss, 1981

34. What about the worms? rice stalk borer Sugarcane borer Often, live specimens are easier to identify than preserved. From Stehr’s Immature Insects

35. European corn borer Mexican rice borer Worms, cont. Fall armyworm entweb.clemson.edu/.../pages/EuroCornBorer.htm

36. True flies, mosquitos, midges (order Diptera) www.sfu.ca/~fankbone/ biol/diptera.jpg

37. http:// animals.howstuffworks.com/.../printable Typical fly adult Typical fly larva, maggot http:// www.fishing-hotspot.co.uk/fishing-with-maggots/ Mosquito larva, also a kind of fly Head Thorax Abdomen

39. Like these swan-necked mortise chisels, sorta.

41. Rice panicle mite: family Tarsonemidae Steneotarsonemus spinki Smiley mites Male Female

42. http:// keys.lucidcentral.org/.../Mite_Glossary.htm Drill + syringe on a minute (cellular) level

43. J. Saichuk mites Brown discoloration of sheath

44. www.panoramio.com/photo/1057758

46. The first responder’s toolkit Specimen vials/bottles/ziplocks Labeling supplies Field notebook Preservative: ethanol Knife w/scissors, handlens

51. Questions?