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BioInformatics
   by Nuno Barreto

      SHiFT08
What this presentation is

• A general description of what
  bioinformatics is
• A voyage in most aspects involved
• A view of the technologies used
• A view of the future
What this presentation is
          not


• An exhaustive explanation of what
  Bioinformatics is
Bioinformatics?
Bioinformatics
          =
Molecular Biology + IT
So, that excludes other
interesting applications of IT in
   the Biology related fields
The democratization of Biological Information
Geographic information systems
So...

       What is Bioinformatics?

         What is it used for?

Why has it changed the face of Biology?
It’s all about DNA
• DNA is a nucleic acid that contains a
  “blueprint”

• This “blueprint” is used to construct every
  part of the organism, it is all Nature needs
  to reproduce an organism and keep it
  functioning correctly
image from Wikipedia
How is it captured?
Photo from http://www.flickr.com/photos/gravitywave/7715395/
Image from http://www.dna11.com
images from Wikipedia
image from Wikipedia
Made at http://www.baekdal.com/web2dna
In fact, it’s all about String Manipulation




                                              image from Wikipedia
Alignment of 27 avian influenza hemagglutinin protein
                     sequences




                                              image from Wikipedia
Genomes
• Genomes are the complete set of
  chromosomes of organism, which contain
  its full DNA

• It includes genes
• Delimitations
• And also “junk DNA”
Genes


• “a gene is a portion of an organism's DNA
  which contains both quot;codingquot; sequences
  that determine what the gene does, and
  quot;non-codingquot; sequences that determine
  when the gene is active” (from Wikipedia)
image from Wikipedia
Human Genome


• 23 chromosome
  pairs
• 3.2 billion DNA
  base pairs
• 20 500 genes

                         image from Wikipedia
Chicken Genome



• 1 billion DNA base
  pairs
• 20-23 000 genes
                       Photo from http://www.flickr.com/photos/thomashawk/31562846/
Amoeba dubia Genome




• 670 billion DNA
  base pairs


                    photo from www.behav.org/GENE/org/org_040_C.htm
Only 79 eukaryotic genomes

Only ~400 prokaryotic genomes

   Only 27 archaeal genomes
Still a lot of genomes to be sequenced,
      considering the 1.8 million classified species,
and the possibility of existing 10 to 100 million unknown.
Proteomics
• It’s all about discovering the sets of
  proteins of an organism, knowing it’s
  function
Protein Folding
• RNA is the blueprint of Proteins
• Proteins structure is very important for it’s
  functioning

• Therefore, it is crucial to know its shape in
  order to know its function
Protein Folding




                  image from Wikipedia
Hemoglobin




             image from Wikipedia
Folding @home
http://folding.stanford.edu/
Tree of Life
Bioinformatics
Bioinformatics
Bioinformatics
Bioinformatics
All info available through websites
http://www.uniprot.org
Bioinformatics
Bioinformatics
Bioinformatics
http://www.ebi.ac.uk/Tools/clustalw2/index.html
Bioinformatics
Bioinformatics
What can we do with all this
       information?
Better understand all organisms
Most importantly,
we can understand our bodies better
It is a great advancement in

    medicine
• Helps in disease diagnosis and prevention
• Helps understand our response to
  treatments, creating the possibility of
  customized ones

• Identification of drug targets
• Gene Therapy
Future
• More advances in specific treatment
• A better comprehension of our bodies
• A better comprehension of the
  relationships between organisms

• Who knows? :)
nbarreto@gmail.com

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Bioinformatics