Frozen tuna can be safely defrosted and revived in the refrigerator overnight or by submerging it in cold water. For the refrigerator method, place the frozen tuna on a plate and let it thaw in the fridge for 8-12 hours. For cold water defrosting, place the tuna in a container and cover with cold water, changing the water every 30 minutes until thawed, which usually takes 1-2 hours.