2. Scope of the guidelines
Aims to describe quantitative PCR.
Technical guideline to create a meaningful
RDML file correctly (overcome limits of XML).
Extended/replaced by MIQE in future.
3. Level of implementation
MIqPCR was published with RDML v1.0.
MIQE is in the focus of the journals.
qPCR-Ninja is developed to support RDML and
MIQE.
6. Scope of the guidelines
MIQE - minimum information for publication of
quantitative real-time PCR experiments
entire qPCR workflow
experimental design
sample information
sample processing
target information
oligos and probes
qPCR protocol
qPCR validation
data analysis
7. Level of implementation
creation of public awareness
presentations on numerous scientific meetings
company tours
part of qPCR courses
letter to editors
follow up papers
standard data format RDML (listed as MIqPCR @
MIBBI)
cited in 185 papers since 2009
guidelines adopted by journals
Clinical Chemistry
Nucleic Acids Research
BMC-Series journals
8. RDML
1.0 since 2008
1.1 to be released soon
2.0 planned
Taken up by different companies
Life Technologies
Bio-Rad
Biogazelle
9. Status & funding
Continued effort to create public awareness
Active discussion with industry on what is acceptable
under the MIQE label
Planning for MIQE 2.0
Strengthen link with RDML
Enhance development of website
No dedicated MIQE funding