1. High Throughput Screening
(HTS)
Submitted to: Submitted by:
Mr. L.Ratnakar Singh Manish Kumar
M.Pharm (Pharmacology)
Department of Pharmacy , Institute of Bio-Medical Education & Research,
Mangalayatan university Beswan, Aligarh
2. HIGH THROUGHPUT SCREENING (HTS)
Identification of one or more positive candidates
extracted from a pool of possible candidates
based on specific criteria.
3. INTRODUCTION
High Throughput Screening (HTS) is a
drug-discovery process widely used in the
pharmaceutical industry. It leverages
automation to quickly assay the biological
or biochemical activity of a large number
of drug-like compounds.
4. It is a useful for discovering ligands for
receptors, enzymes, ion-channels or other
pharmacological targets, or pharmacologically
profiling a cellular or biochemical pathway of
interest. Typically, HTS assays are performed
in "automation-friendly" microtiter plates with
a 96, 384 or 1536 well format.
5. Assay Technology in HTS
Cell growth tests (cell-based assays or
Phenotypic assays)
Tissue response - targeted functional cell-
based assay
Enzyme test - biochemical test
6. ADVANTAGES OF HTS
High sensitivity of assay (single molecule detection)
High speed of assay (automation)
Minimization of assay (microtiter plate assay)
Low background signal
Clear message (best: Yes/No answer)
7. Low complexity of assay (specific interaction)
Reproducibility
Fast data processing of results
Acceptable costs !!!
12. MICROSCOPY IN HTS:
• Scanning Tunnelling Microscopy
• Atomic Force Microscopy
• Confocal Microscopy
13. USES OF HTS:
To screen for all kind of novel biological active compounds
(libraries):
• Natural products
• Combinatorial Libraries (peptides, chemicals…)
• Biological libraries
To screen Micro arrays such as:
• DNA chips
• RNA chips
• Protein chips