2. Prokaryotic Cells
• Comparing Prokaryotic and Eukaryotic
Cells
– Prokaryote comes from the Greek words
for prenucleus.
– Eukaryote comes from the Greek words
for true nucleus.
3. Prokaryote Eukaryote
• One circular • Paired
chromosome, not in chromosomes, in
a membrane nuclear membrane
• No histones • Histones
• No organelles • Organelles
• Peptidoglycan cell • Polysaccharide cell
walls walls
• Binary fission • Mitotic spindle
4. Glycocalyx
• Outside cell wall
• Usually sticky
• A capsule is neatly
organized
• A slime layer is
unorganized & loose
• Extracellular
polysaccharide
allows cell to attach
• Capsules prevent
phagocytosis
9. Gram-Positive cell walls
• Teichoic acids:
– Lipoteichoic acid links to plasma membrane
– Wall teichoic acid links to peptidoglycan
• May regulate movement of cations
• Polysaccharides provide antigenic variation
10. Gram-Negative Outer Membrane
• Lipopolysaccharides, lipoproteins, phospholipids.
• Forms the periplasm between the outer membrane and
the plasma membrane.
• Protection from phagocytes, complement, antibiotics.
• O polysaccharide antigen, e.g., E. coli O157:H7.
• Lipid A is an endotoxin.
• Porins (proteins) form channels through membrane
12. Gram Stain Mechanism
• Crystal violet-iodine crystals form in cell
• Gram-positive
– Alcohol dehydrates peptidoglycan
– CV-I crystals do not leave
• Gram-negative
– Alcohol dissolves outer membrane and leaves holes
in peptidoglycan
– CV-I washes out
13. A typical Cell Walls
• Mycoplasmas
– Lack cell walls
– Sterols in plasma membrane
• Archaea
– Wall-less, or
– Walls of pseudomurein (lack NAM and D amino acids)
14. Damage to Cell Walls
• Lysozyme digests disaccharide in peptidoglycan.
• Penicillin inhibits peptide bridges in peptidoglycan.
• Protoplast is a wall-less cell.
• Spheroplast is a wall-less Gram-positive cell.
• L forms are wall-less cells that swell into irregular
shapes.
• Protoplasts and spheroplasts are susceptible to
osmotic lysis.
15. Fimbriae & Pili
• Fimbriae allow
attachment
• Pili are used to
transfer DNA from
one cell to another
Fimbriae
200 nm
16. Flagella
• Outside cell wall
• Made of chains of
flagellin
• Attached to a protein
hook
• Anchored to the wall
and membrane by
the basal body
19. Motile Cells
• Rotate flagella to run or tumble
• Move toward or away from stimuli (taxis)
• Flagella proteins are H antigens
(e.g., E. coli O157:H7)
23. Fluid Mosaic Model
• Membrane is as viscous as olive oil.
• Proteins move to function
• Phospholipids rotate and move
laterally
24. • Some prokaryotes
– Do have specialized membranes that perform
metabolic functions
0.2 m 1 m
Respiratory
membrane
Thylakoid
membranes
(a) Aerobic prokaryote (b) Photosynthetic prokaryote
25. Nucleoid
• The typical prokaryotic genome
– Is a ring of DNA that is not surrounded by a
membrane and that is located in a nucleoid region
• Some species of bacteria
– Also have smaller rings of DNA called plasmids
Chromosome
1 m
26. Endospores
• Resting cells
• Resistant to desiccation, heat, chemicals
• Bacillus, Clostridium
• Sporulation: Endospore formation
• Germination: Return to vegetative state
32. Type of Bacteria: Nutrition and metabolic adaptation
• A great diversity of nutritional and metabolic
adaptations have evolved in prokaryotes
• Examples of all four models of nutrition are found
among prokaryotes
– Photoautotrophy
– Chemoautotrophy
– Photoheterotrophy
– Chemoheterotrophy
33. Type of Bacteria: Nutrition and metabolic adaptation
• Major nutritional modes in prokaryotes
34. Type of Bacteria: Metabolic Relationships to Oxygen
• Obligate aerobes
– Require oxygen
• Facultative anaerobes
– Can survive with or without oxygen
• Obligate anaerobes
– Are poisoned by oxygen
47. Classification: Subkingdom Archaebacteria
Crenarchaeota (Thermophile & Acidophile)
P. furiosus (archaea)
• In the marine sand surrounding
sulfurous volcanoes.
• Optimally at 100°C (212°F)
• Highly resistant to radiation
• May possess an efficient
system for repairing DNA.
50. Classification: Subkingdom Eubacteria
Clamydias
• Chlamydia trachomatis
- May be transmitted to newborn's eyes
- Painful urination and watery discharge
• Mycoplasma hominis
• Ureaplasma urealyticum
51. Nongonococcal Urethritis
• Chlamydia trachomatis
– May be transmitted to newborn's eyes
– Painful urination and watery discharge
• Mycoplasma hominis
• Ureaplasma urealyticum
52. Gonorrhea
• Neisseria gonorrhoeae
• Attaches to oral or urogenital mucosa by fimbriae
• Females may be asymptomatic; males have painful
urination and pus discharge
• Treatment with antibiotics
• Untreated may result in
– Endocarditis
– Meningitis
– Arthritis
– Ophthalmia neonatorum
58. Classification
I. Cyanophyta Blue green algae (Cyanobacteria)
Morphology
• Non nuclear membrane
• Cell wall ; Cellulose & Pectin
• Non flagella
• Cyanophycean Starch
• Pigment : chlorophyll a , B - carotene , Flavicin &
phycobilin 2 type ; c - phycoerythrin & c - phycocyanin
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