Surface Plasmon Resonance,
Surface Plasmons:
Plasmons confined to surface (interface) and interact with light resulting in polarities.
Propagating electron density waves occurring at the interface between metal and dielectric.
3.
Surface Plasmons:
Plasmons confined to surface (interface) and interact
with light resulting in polarities.
Propagating electron density waves occurring at the
interface between metal and dielectric.
What is SPR?
10.
How does SPR work?
This Kretschmann Experimental
System uses a metal film thin
enough to monitor the plasmon
A plasmon can be thought of as a
ray of light bound onto a surface -
propagating among the surface
and presenting itself as an
electromagnetic field.
11.
SPR & Refractive Index
In optics the refractive index or index of
refraction n of an optical medium is a
dimensionless number that describes how
light, or any other radiation, propagates
through that medium.
12.
Applications of SPR
Physical applications: measure
dielectric properties, adsorption
processes, surface degradation or
hydration of
Thin organic monolayers or
bilayers
Polymer films
13.
Applications of SPR
Biological applications: as biosensors for
specific biological interactions including
adsorption and desorption kinetics, antigen-
antibody binding and epitope mapping for
determination of
Biomolecular structure and interactions of
proteins, DNA & Viruses
Lipid Bilayers
Non-specific biomolecular interactions-
bio-compatibility
Tissue engineering
14.
SPR imaging apparatus
Reflected light from the
gold surface, containing the
SPR image, is monitored
with a CCD camera.
The angle of incidence can
be changed by rotating the
entire sample assembly.