Kartheek Dokka=Chap23

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The document summarizes research on identifying cells that initiate human malignant melanoma (MMICs). The key findings are: 1. ABCB5, an ATP-binding cassette transporter protein, is expressed in MMICs and closely associated with advanced melanoma. 2. ABCB5+ melanoma cells were able to initiate tumor formation in mice, while ABCB5- cells did not, indicating ABCB5+ cells drive melanoma progression. 3. Blocking ABCB5 using an antibody inhibited tumor growth in mice xenografts, suggesting ABCB5 is a potential therapeutic target for treating advanced melanoma.

Saúde e medicina Tecnologia

Identification of Cells initiating Human Melanoma 17-January-08 Nature-06489 Kartheek Dokka

The Target! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],MMIC ABCB5

Relation of ABCB5 with malignant melanoma ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],A B D C green orange violet blue pink yellow

Expression of other markers/identifiers ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Subset defined by ABCB5 ,[object Object],[object Object]

ABCB5+ vs ABCB5 ¯ 1 ° patient tumor cells NOD/SCID mice XenoTransplants ABCB5+ ABCB5- Unsegregated Repurification Secondary Tumor Formation Total: US -7/23, + :14/23, - :1/23 + :10/18 - :0/18 Immuno-Magnetic Separation

ImmunoHistoChemistry usage for Progression of ABCB5 ,[object Object],[object Object],[object Object]

Flow Cytometry readings for ABCB5 progression ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Final Experiment with “nude mouse” ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Welcome to K-Lab! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

My results! July 13-2021 Time: 11:30pm ,[object Object],[object Object],[object Object],[object Object],Tumor Formation Time Tumor Formation Time Xenotransplants with RNAi - As the gene was silenced, tumor formation was suppressed for 3 hours and as the ABCB5 was re-made, tumor was induced. Controls: Positive: Healthy Human cells Negative: Melanoma cells of primary human patients.

Experiment 2: Chemical Carcinogen Patch ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],15 th July 2021-3 rd August 2001 9:00Am 10:35pm Tumor Formation Tumor Formation Tumor Formation Time [days] Time [days] Time [days]

References ,[object Object],[object Object],[object Object],[object Object],[object Object]

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