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1. Nanotechnology Meets Biology:
Peptide-based Methods for the
Fabrication of Functional Materials
Beverly D. Briggs and Marc R. Knecht
University of Miami
J. Phys. Chem. Lett. 2012, 3, 405-418. 1

2. Motivation
• Mimicking Nature’s specificity, size
and shape control of nanoparticle
(NP) formation can be achieved.
• Through this control, nanoparticle
synthesis can be engineered for
specialized applications.
• Traditional methods for
nanoparticle fabrication include
high temperatures and harsh
conditions.
• Biomimetic techniques offer eco-
friendly methods for fabrication
under ambient conditions and
temperatures.
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3. Cell Surface/Phage Display
 Biocombinatorial isolation methods are utilized to identify peptides that
bind to non-natural targets.
Sarikaya, et. al.,
Nat. Mater., 2003
 These techniques allow for identification of peptides with the ability to bind
materials for technological applications.
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4. Palladium and Platinum
Both Pd and Pt have applications in
catalysis, energy storage, and gas
sensing.
• Q7 and Pd4 – spherical NP size control,
isolated with Pd specificity.
• R5 – originally isolated for SiO2 from
diatoms, but also acts as a template for
shaped particle formation.
Chiu, C.-Y., et. al., Nanoscale, 2010.
Bhandari, R., et. al., ACS Catal., 2011.
• BP7A – preferentially binds to {110}
surface, forming multipods.
• T7 and S7 – preferentially bind to
{100} and {111}, respectively, controls
NP shape as well.
• Cha – peptide binds to {100} and
forms nanocubes.
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Chiu, C., et. al., Nat. Chem., 2011.

5. Gold and Silver
Carter, C. J.,
Au and Ag NPs possess visible et. al., ACS
plasmons, making them relevant for Nano, 2010.
optics, biosensing, and catalysis.
• E5 and FlgA3 – can impart chiral properties on achiral
Au particles.
• A3 – conjugated with organic molecules, leads to self
assembly of unique Au NP helices and hollow spheres.
• AG4 – used to create hybrid Au peptide spheres.
• E6, D6, and EEEE – generates Ag particles that
require only light for reduction and act as a template
for nanowires in Li ion batteries.
• Ge8 – mediates the formation of Ag nanoparticles,
which, when immobilized, result in curled structures.
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Chen, C.-L., et. al., J. Am. Chem. Soc., 2008.
Song, C., et. al., J. Am. Chem. Soc., 2010.

6. Metal Oxides
The electronic structure of
ZnO, TiO2, and BaTiO3 make
them applicable for optics,
photovoltaics, and catalysis.
• Z1 – shown to form ZnO hexagonal
plates without secondary reagents.
• G-12 and GT-16 – shown to preferentially
bind to the (0001) ZnO surface.
Ahmad, G., et. al., J. Am. Chem. Soc., 2008.
• Ti-1 and Ti-1 (RKK) –
demonstrates that basic residues • BT1 and BT2 – revealed to form
are important in TiO2 NP formation. tetragonal BaTiO3 crystals with
• Ti (Si) – 1 – isolated to bind and ferroelectric properties.
generate TiO2 and not SiO2.
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7. Conclusions:
Acknowledgments:
• Peptides can mediate the We graciously acknowledge the
formation of a variety of National Science Foundation
(Grants DMR-1145175 and
technologically significant CBET-1157431) for financial
materials. assistance. Further support from
• Composition-specific the University of Miami is also
acknowledged.
interactions are important
for NP formation, although
peptide templates can also
be used.
• New techniques are
needed to further probe the
properties of biomimetic
particles.
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