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Dr H.D. Palekar  (M.S.) Associate Professor Dept of Surgery Medical College Bhavnagar Sterilization By
OUT LINE ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Definitions:- ,[object Object],[object Object],[object Object],[object Object],[object Object]
Definitions:- ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Disinfection:-
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Definitions:-
Definitions:- ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],Definitions:-
HISTORY :- ,[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],HISTORY :-
[object Object],[object Object],[object Object],[object Object],HISTORY :-
HISTORY :- ,[object Object],[object Object]
[object Object],[object Object],[object Object],HISTORY :- AUGUSTINO  BASSI   (1835)  :-   Muscardin disease of silk worm was  caused by a fungus.
HISTORY :- ,[object Object],[object Object],[object Object]
HISTORY :- ,[object Object],[object Object]
HISTORY :- ,[object Object]
[object Object],[object Object],[object Object],HISTORY :-
HISTORY :- ,[object Object]
[object Object],[object Object],[object Object],HISTORY :-
CLASSIFICATION Methods Physical Chemical Heat Radiation Filtration Ultrasonic Vibrator Dry Heat Moist Heat Non Ionizing Ionizing Earthenware Asbestos Disc Sintered Glass Collodion( Membrane) Slow sand & Rapid
Physical Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods
[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods Precautions:-
[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],  Physical Methods
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods At a Temperature Above 100’C
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods USES of autoclave
[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods To Sterilize( I R )
[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Physical Methods
Chemical Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Chemical Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Chemical Methods ,[object Object],Most IMP Surfece Acting Agents Anionic Cationic Nonionic Amphoteric Common Soap Cetrimide  Or Cetavlon Polysorbates Tego compound
Chemical Methods ,[object Object],Phenol Carbolic Acid Cresol ( LYSOL )  Chloroxylenol (DETTOL) Chlorhexidine Chlorocresol
Chemical Methods ,[object Object],[object Object],[object Object]
Chemical Methods ,[object Object],[object Object],[object Object],[object Object]
Chemical Methods ,[object Object],Dyes Acriflavin Proflavin Methylene Blue Crystal Violet Brilliant Green
Chemical Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. 3. Glutaraldehyde 2%  10-30 min ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. 7 .  Chlorhexidine   2min Cell wall membrane damage. (to microorganism) 8.   Cresol ( LYSOL) Cell wall membrane damage. ( to microorganism) ,[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. 11 .  Bleach 1%  Solution 12 . Bleaching Powder ,[object Object],[object Object]
Commonly Used Chemical Disinfectants. 13 .  Basillol-25 ,[object Object],[object Object],[object Object],Disinfection of electronic Equipments in:    OTs    ICU    NICU    Recovery room    Medical Laboratories It is preferred disinfectant for :    Bronchoscopy    Ophthalmology    Dentistry
Commonly Used Chemical Disinfectants. ,[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Commonly Used Chemical Disinfectants.
Commonly Used Chemical Disinfectants. korsolex Strength of solution Immersion Time Instruments High Level disinf. ( Standard disinfe).   5% Bacterial Spores X 30 min-1hr only (When speed of action is the need of the hour) Surgical Instruments Emergency disinfection 10% 15 min only All Endoscopes-Laparoscopes Total sterilization ( Sporicidal disinfection) 10% / 5 % 5 hrs / 10 hrs All implants & surgical items
Levels of disinfection ,[object Object],[object Object],[object Object],[object Object]
Levels of disinfections ,[object Object],[object Object]
Levels of disinfections ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Levels of disinfections ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Levels of disinfections ,[object Object],[object Object],[object Object],[object Object],[object Object]
Levels of disinfections ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Levels of disinfections Levels of disinfection Items Time  disinfectant High level Critical and semi critical items (except thermometers and hydrotherapy tanks) >=20min. (sterilization  X , then HLD) Gluteraldehyde, Hydrogen peroxide, peracetic acid , peracetic acid with hydrogen peroxide, chlorines Intermediate level Semicritical and noncritical(except environmental surfaces) <=10min. Alchohols, Iodophors, Phenolics, chlorines Low level(environmental surfaces) Noncritical  Alchohols, Iodophors, Phenolics, chlorines
Commonly Used Chemical Disinfectants. korsolex
[object Object],[object Object],[object Object],Commonly Used Chemical Disinfectants.
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Commonly Used Chemical Disinfectants.
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Commonly Used Chemical Disinfectants. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Sterilization- Methods Of Choice 
 
 
Cleaning and disinfection, Commonly used in the hospital General Use articles. √   Bath water   -  Add Savlon when necessary √   Bed Pans  - Wash with hot water and keep dry.   - Disinfect with phenol after used by infected patients. √   Bowls   - Autoclave/ wash with hot water and keep dry. √   Crockery. Cutlery  – Wash with hot water and detergent. Keep dry. √   Floors   - Vacuum clean      No Broom to be used √   Furniture, Bed Frames -   Damp dust with detergent or with    phenol or with 2% Lysol solution √   Mattresses/Pillows –  Cover with water-impermeable cover. Wash cover  with detergent solution and dry. Disinfect with  phenol when necessary. √   Trolley tops.  –  Wipe with warm water and detergent to remove  dust and keep dry.
√   Thermometers  –  Wash with warm water and detergent or 70% alcohol for    one minute and keep dry. √   Endoscopes/ Arthroscopes / Laparoscopes / Fiberoptic Endoscopes.   -  Immerse in 2% alkaline glutaradhyde (CIDEX) under strict    controlled conditions. As per manufactures instructions. Alternative Use-   - Ethylene oxide sterilization   - Alcohol disinfection   - Hydrogen peroxide and peracetic acid √   Endotracheal suction catheter   -  Should be disposable   - Or may be stored for 24 hours properly so that it  does not  get contaminated   -Flush catheter with sterile distilled water each use.   - Bowl must be washed and dried after each suction.
√   Endotracheal tubes   -  Recycled after cleaning and autoclaving √   Ambu Bags.   -  Ideally heat disinfect   -  Or immerse in 2% glutaraldhyde and then    wash with sterile distilled water to reduce to    risk of chemical irritation which can precipitate    respiratory infection. √   Oxygen delivery face mask   -  Wash and then dry. - Wipe with 70% isopropyl alcohol to remove    mucus. √   Suction drainage bottles -  Ideally autoclave √   Ventilatory circuits respiratory equipment in  neonatal/pediatric unit   -  Heat disinfection at 80’C for 3 minutes.  - Or autoclave - Or Ethylene oxide sterilization.
   Do not use these instruments for more than 72 hours  without sterilization √  Incubators  - Clean thoroughly with warm water and soap - Wipe with 70% Isopropyl alcohol √   Humidifiers - Empty daily - Refill with sterile water - Disinfect when contaminated, with 1%    hypochlorite for 30 minutes, wash and dry - Autoclave after each patient’s use - Autoclave when respiratory circuit is changed √   Urinary catheter - Ideally single use – disposable.
Operation Theater Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Operation Theater Protocol ,[object Object],[object Object]
Operation Theater Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],Cruse PJ, Foord R. A five-year prospective study of 23,649 surgical wounds. Arch Surg. 1973 Aug;107(2):206-10. N = 23,649  if shaved    ->  ->   2.3% infection rate if no hair removed   ->  ->   0.9% infection rate
 
Operation Theater Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Operation Theatre Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Operation Theatre Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Operation Theatre Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
HAND WASH
HAND WASH   RESIDENT FLORA ,[object Object],[object Object],[object Object],Coagulation negative staphylococci, micrococci, and coreni bacteria TRANSIENT FLORA (contact organisms) ,[object Object],[object Object],Gram negative, aerobic spore formers fungi and viruses. TEMPORORY  RESIDENT FLORA   ,[object Object],Staph aureus INFECTION FLORA ,[object Object],[object Object],Staph aureus,  β  haemolytic streptococci
HAND WASH √  Three Types of Hand washing: (1) Social Hand Washing (2) Hygienic Hand Washing (3) Surgical Hand Washing      Scrub Method      Rub Method
HAND WASH ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
HAND WASH ,[object Object]
HAND WASH ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
HAND WASH ,[object Object]
HAND WASH Social & Hygienic Hand Wash
 
 
HAND WASH ,[object Object],Rub Method Scrub Method
HAND WASH ,[object Object],SCRUB   METHOD :
HAND WASH ,[object Object]
HAND WASH ,[object Object]
 
 
 
 
 
 
HAND WASH (SCRUB METHOD) ,[object Object],[object Object],[object Object],[object Object]
HAND WASH ,[object Object]
 
 
 
 
 
 
STERILLIUM ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Rub method :
HAND WASH ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
HAND WASH
GOWNING
GOWNING
Operation Theatre Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Operation Theatre Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Operation Theatre Protocol ,[object Object],[object Object]
Operation Theatre Protocol ,[object Object],[object Object]
Operation Theatre Protocol ,[object Object],[object Object],[object Object],[object Object],[object Object]
Operation Theatre Protocol OT Sterilization
Operation Theatre Protocol ,[object Object],[object Object],[object Object]
OT Sterilization   (2)  BACILLOCID SPECIAL ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
OT Protocol ,[object Object]
 
 
OT Protocol ,[object Object]
 
 
MANAGEMENT OF SPILLS
 
 
 
[object Object],[object Object],[object Object],[object Object]
Thank   You

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Sterilization

  • 1. Dr H.D. Palekar (M.S.) Associate Professor Dept of Surgery Medical College Bhavnagar Sterilization By
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  • 19. CLASSIFICATION Methods Physical Chemical Heat Radiation Filtration Ultrasonic Vibrator Dry Heat Moist Heat Non Ionizing Ionizing Earthenware Asbestos Disc Sintered Glass Collodion( Membrane) Slow sand & Rapid
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  • 56. Commonly Used Chemical Disinfectants. korsolex Strength of solution Immersion Time Instruments High Level disinf. ( Standard disinfe). 5% Bacterial Spores X 30 min-1hr only (When speed of action is the need of the hour) Surgical Instruments Emergency disinfection 10% 15 min only All Endoscopes-Laparoscopes Total sterilization ( Sporicidal disinfection) 10% / 5 % 5 hrs / 10 hrs All implants & surgical items
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  • 63. Levels of disinfections Levels of disinfection Items Time disinfectant High level Critical and semi critical items (except thermometers and hydrotherapy tanks) >=20min. (sterilization X , then HLD) Gluteraldehyde, Hydrogen peroxide, peracetic acid , peracetic acid with hydrogen peroxide, chlorines Intermediate level Semicritical and noncritical(except environmental surfaces) <=10min. Alchohols, Iodophors, Phenolics, chlorines Low level(environmental surfaces) Noncritical Alchohols, Iodophors, Phenolics, chlorines
  • 64. Commonly Used Chemical Disinfectants. korsolex
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  • 73. Cleaning and disinfection, Commonly used in the hospital General Use articles. √ Bath water - Add Savlon when necessary √ Bed Pans - Wash with hot water and keep dry. - Disinfect with phenol after used by infected patients. √ Bowls - Autoclave/ wash with hot water and keep dry. √ Crockery. Cutlery – Wash with hot water and detergent. Keep dry. √ Floors - Vacuum clean  No Broom to be used √ Furniture, Bed Frames - Damp dust with detergent or with phenol or with 2% Lysol solution √ Mattresses/Pillows – Cover with water-impermeable cover. Wash cover with detergent solution and dry. Disinfect with phenol when necessary. √ Trolley tops. – Wipe with warm water and detergent to remove dust and keep dry.
  • 74. Thermometers – Wash with warm water and detergent or 70% alcohol for one minute and keep dry. √ Endoscopes/ Arthroscopes / Laparoscopes / Fiberoptic Endoscopes. - Immerse in 2% alkaline glutaradhyde (CIDEX) under strict controlled conditions. As per manufactures instructions. Alternative Use- - Ethylene oxide sterilization - Alcohol disinfection - Hydrogen peroxide and peracetic acid √ Endotracheal suction catheter - Should be disposable - Or may be stored for 24 hours properly so that it does not get contaminated -Flush catheter with sterile distilled water each use. - Bowl must be washed and dried after each suction.
  • 75. Endotracheal tubes - Recycled after cleaning and autoclaving √ Ambu Bags. - Ideally heat disinfect - Or immerse in 2% glutaraldhyde and then wash with sterile distilled water to reduce to risk of chemical irritation which can precipitate respiratory infection. √ Oxygen delivery face mask - Wash and then dry. - Wipe with 70% isopropyl alcohol to remove mucus. √ Suction drainage bottles - Ideally autoclave √ Ventilatory circuits respiratory equipment in neonatal/pediatric unit - Heat disinfection at 80’C for 3 minutes. - Or autoclave - Or Ethylene oxide sterilization.
  • 76. Do not use these instruments for more than 72 hours without sterilization √ Incubators - Clean thoroughly with warm water and soap - Wipe with 70% Isopropyl alcohol √ Humidifiers - Empty daily - Refill with sterile water - Disinfect when contaminated, with 1% hypochlorite for 30 minutes, wash and dry - Autoclave after each patient’s use - Autoclave when respiratory circuit is changed √ Urinary catheter - Ideally single use – disposable.
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  • 87. HAND WASH √ Three Types of Hand washing: (1) Social Hand Washing (2) Hygienic Hand Washing (3) Surgical Hand Washing  Scrub Method  Rub Method
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  • 92. HAND WASH Social & Hygienic Hand Wash
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  • 123. Operation Theatre Protocol OT Sterilization
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  • 137. Thank You