SlideShare a Scribd company logo

Detection of transgenic canola (Roundup Ready® - Monsanto)

•
•
claudio iannetta
claudio iannetta

Determination of a validation protocol, based on established European Union methods, for the detection of transgenic canola (Roundup Ready® - Monsanto) in seed samples using molecular techniques

Technology
1 of 41
Claudio Iannetta (s3154021) Supervisor: Saman Buddhadasa Senior Food Scientist Food Metrology National Measurement Institute
PROJECT ,[object Object],[object Object],[object Object],[object Object],[object Object]
AGENDA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
About Canola ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],(www.whitemoldresearch.com)
GM Canola (1) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],GM Canola (2) (www.biotechnologie.it)
GM Canola (3) ,[object Object],[object Object],[object Object],[object Object]
Health Risks Associated ,[object Object],[object Object],[object Object],[object Object]
Health Risks Associated ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
International Legislation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Australian Legislation (1)
Australian Legislation (2) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Detection Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DNA Based Methods ,[object Object],[object Object],[object Object],[object Object],[object Object]
DNA Based Methods ,[object Object],[object Object],[object Object],[object Object]
Protein Based Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Why real-time PCR? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Project Aims ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Justifications ,[object Object],[object Object],[object Object],[object Object]
Project – Flow Chart ✔ ✔ ✔
Grinding of the Seed Samples ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DNA Isolation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DNA Concentration ,[object Object],[object Object],[object Object],A 260 DNA Concentration 0.0122 37 ng/µL 0.0114
DNA Purity ,[object Object],[object Object],[object Object],[object Object],A 260 A 280 Average: A 260 /A 280 0.0122 0.0076 1.81 0.0114 0.0054
DNA Fragmentation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Conventional PCR (1) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Conventional PCR (2) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Stage Cycle No Setting C 1 1 94 1 ' 2 40 94 1 ' 47 1 ' 72 30 '' 3 1 72 7 ' 4 1 4 ∞
PCR Agarose Gel Electrophoresis ,[object Object],[object Object],[object Object],[object Object]
Real-time PCR (1) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Real-time PCR (1) ,[object Object],[object Object],[object Object],[object Object],[object Object]
Real-time PCR (2) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Real-time PCR (3) ,[object Object],[object Object],[object Object],[object Object],Stage Cycle No Setting C 1 1 1 1 ,[object Object],[object Object],2 40 95 15 '' 60 1 ' 4 1 4 ∞
96 Well PCR Plate ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],The plate is split in two identical parts and the difference in some of the reagents enables the detection of the total amount of canola or the transgenic one
Standard Curve WT Canola ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Standard Curve GM Canola ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Problems with Standard Curve ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Data Analysis ,[object Object],[object Object],[object Object],[object Object],Expected % Determined % Mean Standard Deviation Coefficient Variation <25% 2 0.90 0.54% 0.005 92.1% 0.19 1 0.07 0.04% 0.004 106.1% 0.01
Some Good Results ( FatA ) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Data Analysis The consistency of the results is assured if Coefficient of Variation is below 25%, which is the recommended value from EU Network of GMO Laboratories Sample A Sample B E ( FatA ) R 2 ( FatA ) 1 2.18 x 10 3 917.891 95% 99% 2 2.26 x 10 3 840.949 3 2.24 x 10 3 879.143 Mean 2.23 x 10 3 879.327 SD 41.63 38.47 CV 1.87% 4.37%
Conclusions ,[object Object],[object Object],[object Object]
References

Recommended

Nutrition and feeding of native pig
Nutrition and feeding of native pigNutrition and feeding of native pig
Nutrition and feeding of native pig
Perez Eric
 
Postharvest handling and technologies for livestock products
Postharvest handling and technologies for livestock productsPostharvest handling and technologies for livestock products
Postharvest handling and technologies for livestock products
ILRI
 
local and global allignment
local and global allignmentlocal and global allignment
local and global allignment
SHOBI KHAKHI
 
Bio business and biosafety
Bio business and biosafetyBio business and biosafety
Bio business and biosafety
Promila Sheoran
 
Careers in biotechnology
Careers in biotechnologyCareers in biotechnology
Careers in biotechnology
OlaoluMatemilola
 
Animal husbandry
Animal husbandryAnimal husbandry
Animal husbandry
Dr.Amit Hemant Mishal
 
BioEntrepreneurship: The Business of Biotech
BioEntrepreneurship: The Business of BiotechBioEntrepreneurship: The Business of Biotech
BioEntrepreneurship: The Business of Biotech
MaRS Discovery District
 
Genetics Intro Powerpoint
Genetics Intro PowerpointGenetics Intro Powerpoint
Genetics Intro Powerpoint
swidzith
 

Recommended

Nutrition and feeding of native pig
Nutrition and feeding of native pigNutrition and feeding of native pig
Nutrition and feeding of native pig
Perez Eric
 
Postharvest handling and technologies for livestock products
Postharvest handling and technologies for livestock productsPostharvest handling and technologies for livestock products
Postharvest handling and technologies for livestock products
ILRI
 
local and global allignment
local and global allignmentlocal and global allignment
local and global allignment
SHOBI KHAKHI
 
Bio business and biosafety
Bio business and biosafetyBio business and biosafety
Bio business and biosafety
Promila Sheoran
 
Careers in biotechnology
Careers in biotechnologyCareers in biotechnology
Careers in biotechnology
OlaoluMatemilola
 
Animal husbandry
Animal husbandryAnimal husbandry
Animal husbandry
Dr.Amit Hemant Mishal
 
BioEntrepreneurship: The Business of Biotech
BioEntrepreneurship: The Business of BiotechBioEntrepreneurship: The Business of Biotech
BioEntrepreneurship: The Business of Biotech
MaRS Discovery District
 
Genetics Intro Powerpoint
Genetics Intro PowerpointGenetics Intro Powerpoint
Genetics Intro Powerpoint
swidzith
 
Introduction to Bioinformatics
Introduction to BioinformaticsIntroduction to Bioinformatics
Introduction to Bioinformatics
Denis C. Bauer
 
Biotechnologies for animal breeding and coping with climate change
Biotechnologies for animal breeding and coping with climate changeBiotechnologies for animal breeding and coping with climate change
Biotechnologies for animal breeding and coping with climate change
FAO
 
Molecular evolution
Molecular evolutionMolecular evolution
Molecular evolution
manojsiddartha bolthajira
 
Bioentrepreneurship
BioentrepreneurshipBioentrepreneurship
Bioentrepreneurship
Benish
 
Goat and sheep breeeds and breeeding
Goat and sheep breeeds and breeedingGoat and sheep breeeds and breeeding
Goat and sheep breeeds and breeeding
waiton sherekete
 
Inbreeding and outbreeding
Inbreeding and outbreedingInbreeding and outbreeding
Inbreeding and outbreeding
LekshmiJohnson
 
Introduction to-bioentrepreneurship
Introduction to-bioentrepreneurshipIntroduction to-bioentrepreneurship
Introduction to-bioentrepreneurship
Benish
 
DAIRY VISIT REPORT (AMIT) MODIFY.pptx
DAIRY VISIT REPORT (AMIT) MODIFY.pptxDAIRY VISIT REPORT (AMIT) MODIFY.pptx
DAIRY VISIT REPORT (AMIT) MODIFY.pptx
Amit304894
 
Social and ethical issues of genetic engineering
Social and ethical issues of genetic engineeringSocial and ethical issues of genetic engineering
Social and ethical issues of genetic engineering
SMALLYSINHA
 
Phylogenetic tree construction
Phylogenetic tree constructionPhylogenetic tree construction
Phylogenetic tree construction
Uddalok Jana
 
Advanced genetics
Advanced geneticsAdvanced genetics
Advanced genetics
University of Maryland Extension Small Ruminant Program
 
Product of modern biotechnology
Product of modern biotechnologyProduct of modern biotechnology
Product of modern biotechnology
Sekolah Tinggi Farmasi Indonesia
 
Mapping genetic diversity through genetic markers
Mapping genetic diversity through genetic markersMapping genetic diversity through genetic markers
Mapping genetic diversity through genetic markers
Bahauddin Zakariya University lahore
 
protein sequence analysis
protein sequence analysisprotein sequence analysis
protein sequence analysis
RamikaSingla
 
Role of biotechnology in animal feed
Role of biotechnology in animal feedRole of biotechnology in animal feed
Role of biotechnology in animal feed
Batool Abbas
 
Phylogenetic tree
Phylogenetic treePhylogenetic tree
Phylogenetic tree
Sanzid Kawsar
 
Bioentrepreneurship
BioentrepreneurshipBioentrepreneurship
Bioentrepreneurship
Ilika Kaushik
 
Synthetic biology
Synthetic biology Synthetic biology
Synthetic biology
Elham Lasemi
 
Promiscuous enzyme
Promiscuous enzymePromiscuous enzyme
Promiscuous enzyme
Vikrant Rana
 
Introduction to Biotech
Introduction to BiotechIntroduction to Biotech
Introduction to Biotech
LSC-CyFair Library, LIFE Workshops
 
Bioethics and biosafety in biotechnology
Bioethics and biosafety in biotechnologyBioethics and biosafety in biotechnology
Bioethics and biosafety in biotechnology
sanguru1977
 
Intellectual property rights in Agriculture
Intellectual property rights in AgricultureIntellectual property rights in Agriculture
Intellectual property rights in Agriculture
Agri Adhyapak
 

More Related Content

What's hot

What's hot (20)

Introduction to Bioinformatics
Introduction to BioinformaticsIntroduction to Bioinformatics
Introduction to Bioinformatics
 
Biotechnologies for animal breeding and coping with climate change
Biotechnologies for animal breeding and coping with climate changeBiotechnologies for animal breeding and coping with climate change
Biotechnologies for animal breeding and coping with climate change
 
Molecular evolution
Molecular evolutionMolecular evolution
Molecular evolution
 
Bioentrepreneurship
BioentrepreneurshipBioentrepreneurship
Bioentrepreneurship
 
Goat and sheep breeeds and breeeding
Goat and sheep breeeds and breeedingGoat and sheep breeeds and breeeding
Goat and sheep breeeds and breeeding
 
Inbreeding and outbreeding
Inbreeding and outbreedingInbreeding and outbreeding
Inbreeding and outbreeding
 
Introduction to-bioentrepreneurship
Introduction to-bioentrepreneurshipIntroduction to-bioentrepreneurship
Introduction to-bioentrepreneurship
 
DAIRY VISIT REPORT (AMIT) MODIFY.pptx
DAIRY VISIT REPORT (AMIT) MODIFY.pptxDAIRY VISIT REPORT (AMIT) MODIFY.pptx
DAIRY VISIT REPORT (AMIT) MODIFY.pptx
 
Social and ethical issues of genetic engineering
Social and ethical issues of genetic engineeringSocial and ethical issues of genetic engineering
Social and ethical issues of genetic engineering
 
Phylogenetic tree construction
Phylogenetic tree constructionPhylogenetic tree construction
Phylogenetic tree construction
 
Advanced genetics
Advanced geneticsAdvanced genetics
Advanced genetics
 
Product of modern biotechnology
Product of modern biotechnologyProduct of modern biotechnology
Product of modern biotechnology
 
Mapping genetic diversity through genetic markers
Mapping genetic diversity through genetic markersMapping genetic diversity through genetic markers
Mapping genetic diversity through genetic markers
 
protein sequence analysis
protein sequence analysisprotein sequence analysis
protein sequence analysis
 
Role of biotechnology in animal feed
Role of biotechnology in animal feedRole of biotechnology in animal feed
Role of biotechnology in animal feed
 
Phylogenetic tree
Phylogenetic treePhylogenetic tree
Phylogenetic tree
 
Bioentrepreneurship
BioentrepreneurshipBioentrepreneurship
Bioentrepreneurship
 
Synthetic biology
Synthetic biology Synthetic biology
Synthetic biology
 
Promiscuous enzyme
Promiscuous enzymePromiscuous enzyme
Promiscuous enzyme
 
Introduction to Biotech
Introduction to BiotechIntroduction to Biotech
Introduction to Biotech
 

Viewers also liked

Biosafety testing of gm crops icar november 29 2011
Biosafety testing of gm crops icar november 29 2011Biosafety testing of gm crops icar november 29 2011
Biosafety testing of gm crops icar november 29 2011
Tulika Singh
 
Genetically modified food and its consequences on human health and nutrition
Genetically modified food and its consequences on human health and nutritionGenetically modified food and its consequences on human health and nutrition
Genetically modified food and its consequences on human health and nutrition
woolencastle
 
Genetically modified food powerpoint
Genetically modified food powerpointGenetically modified food powerpoint
Genetically modified food powerpoint
kylemoe2
 
Intellectual Property Rights
Intellectual Property RightsIntellectual Property Rights
Intellectual Property Rights
harshhanu
 

Viewers also liked (13)

Bioethics and biosafety in biotechnology
Bioethics and biosafety in biotechnologyBioethics and biosafety in biotechnology
Bioethics and biosafety in biotechnology
 
Intellectual property rights in Agriculture
Intellectual property rights in AgricultureIntellectual property rights in Agriculture
Intellectual property rights in Agriculture
 
Biosafety testing of gm crops icar november 29 2011
Biosafety testing of gm crops icar november 29 2011Biosafety testing of gm crops icar november 29 2011
Biosafety testing of gm crops icar november 29 2011
 
How transgenic plant is used in agricultural field
How transgenic plant is used in agricultural fieldHow transgenic plant is used in agricultural field
How transgenic plant is used in agricultural field
 
A review of change detection techniques
A review of change detection techniques A review of change detection techniques
A review of change detection techniques
 
Genetically modified food and its consequences on human health and nutrition
Genetically modified food and its consequences on human health and nutritionGenetically modified food and its consequences on human health and nutrition
Genetically modified food and its consequences on human health and nutrition
 
TRANSGENIC CROPS
TRANSGENIC CROPSTRANSGENIC CROPS
TRANSGENIC CROPS
 
GMO presentation
GMO presentationGMO presentation
GMO presentation
 
Genetically modified food powerpoint
Genetically modified food powerpointGenetically modified food powerpoint
Genetically modified food powerpoint
 
GM foods
GM foodsGM foods
GM foods
 
analysis of transgenic plants
analysis of transgenic plantsanalysis of transgenic plants
analysis of transgenic plants
 
Gmo's ppt.......
Gmo's ppt.......Gmo's ppt.......
Gmo's ppt.......
 
Intellectual Property Rights
Intellectual Property RightsIntellectual Property Rights
Intellectual Property Rights
 

Similar to Detection of transgenic canola (Roundup Ready® - Monsanto)

11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
Alexander Decker
 
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
Alexander Decker
 
Principle, Procedure and applications of Digital PCR.pptx
Principle, Procedure and applications of Digital PCR.pptxPrinciple, Procedure and applications of Digital PCR.pptx
Principle, Procedure and applications of Digital PCR.pptx
Vikramadityaupmanyu
 
Microarray validation
Microarray validationMicroarray validation
Microarray validation
Elsa von Licy
 
Molecular detection of food borne pathogens-presentation
Molecular detection of food borne pathogens-presentationMolecular detection of food borne pathogens-presentation
Molecular detection of food borne pathogens-presentation
Yakindra Timilsena, PhD
 

Similar to Detection of transgenic canola (Roundup Ready® - Monsanto) (20)

11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
 
Isolation and pcr amplification of genomic dna from traded seeds of nutmeg
Isolation and pcr amplification of genomic dna from traded seeds of nutmegIsolation and pcr amplification of genomic dna from traded seeds of nutmeg
Isolation and pcr amplification of genomic dna from traded seeds of nutmeg
 
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
11.isolation and pcr amplification of genomic dna from traded seeds of nutmeg
 
Principle, Procedure and applications of Digital PCR.pptx
Principle, Procedure and applications of Digital PCR.pptxPrinciple, Procedure and applications of Digital PCR.pptx
Principle, Procedure and applications of Digital PCR.pptx
 
PCR.ppt
PCR.pptPCR.ppt
PCR.ppt
 
Microarray validation
Microarray validationMicroarray validation
Microarray validation
 
RNA Integrity and Quality – Standardize RNA Quality Control
RNA Integrity and Quality – Standardize RNA Quality Control RNA Integrity and Quality – Standardize RNA Quality Control
RNA Integrity and Quality – Standardize RNA Quality Control
 
Molecular detection of food borne pathogens-presentation
Molecular detection of food borne pathogens-presentationMolecular detection of food borne pathogens-presentation
Molecular detection of food borne pathogens-presentation
 
Dna extraction from molluscs -Jackson chary
Dna extraction from molluscs -Jackson charyDna extraction from molluscs -Jackson chary
Dna extraction from molluscs -Jackson chary
 
Barcode Data Standards
Barcode Data Standards Barcode Data Standards
Barcode Data Standards
 
Pcr
PcrPcr
Pcr
 
PCR lecture.ppt
PCR lecture.pptPCR lecture.ppt
PCR lecture.ppt
 
PCR (polymerase chain reaction) and Extraction of DNA from fungal plant patho...
PCR (polymerase chain reaction) and Extraction of DNA from fungal plant patho...PCR (polymerase chain reaction) and Extraction of DNA from fungal plant patho...
PCR (polymerase chain reaction) and Extraction of DNA from fungal plant patho...
 
Assessment of Genetic Diversity in Wheat Genotypes by using ISSR Molecular Ma...
Assessment of Genetic Diversity in Wheat Genotypes by using ISSR Molecular Ma...Assessment of Genetic Diversity in Wheat Genotypes by using ISSR Molecular Ma...
Assessment of Genetic Diversity in Wheat Genotypes by using ISSR Molecular Ma...
 
Publication
PublicationPublication
Publication
 
Detection of Genetically modified plants and Organic Seed production.
Detection of Genetically modified plants and Organic Seed production.Detection of Genetically modified plants and Organic Seed production.
Detection of Genetically modified plants and Organic Seed production.
 
MicroPRO, A Rapid Microbiology Method Based on Flow Cytometry
MicroPRO, A Rapid Microbiology Method Based on Flow CytometryMicroPRO, A Rapid Microbiology Method Based on Flow Cytometry
MicroPRO, A Rapid Microbiology Method Based on Flow Cytometry
 
Advances Of Molecular Genetics Of Poultry
Advances Of Molecular Genetics Of PoultryAdvances Of Molecular Genetics Of Poultry
Advances Of Molecular Genetics Of Poultry
 
Plant Booklet
Plant BookletPlant Booklet
Plant Booklet
 
Mt DNA
Mt DNAMt DNA
Mt DNA
 

Recently uploaded

IAC 2024 - IA Fast Track to Search Focused AI Solutions
IAC 2024 - IA Fast Track to Search Focused AI SolutionsIAC 2024 - IA Fast Track to Search Focused AI Solutions
IAC 2024 - IA Fast Track to Search Focused AI Solutions
Enterprise Knowledge
 
Histor y of HAM Radio presentation slide
Histor y of HAM Radio presentation slideHistor y of HAM Radio presentation slide
Histor y of HAM Radio presentation slide
vu2urc
 
08448380779 Call Girls In Greater Kailash - I Women Seeking Men
08448380779 Call Girls In Greater Kailash - I Women Seeking Men08448380779 Call Girls In Greater Kailash - I Women Seeking Men
08448380779 Call Girls In Greater Kailash - I Women Seeking Men
Delhi Call girls
 
08448380779 Call Girls In Friends Colony Women Seeking Men
08448380779 Call Girls In Friends Colony Women Seeking Men08448380779 Call Girls In Friends Colony Women Seeking Men
08448380779 Call Girls In Friends Colony Women Seeking Men
Delhi Call girls
 
Artificial Intelligence: Facts and Myths
Artificial Intelligence: Facts and MythsArtificial Intelligence: Facts and Myths
Artificial Intelligence: Facts and Myths
Joaquim Jorge
 
EIS-Webinar-Prompt-Knowledge-Eng-2024-04-08.pptx
EIS-Webinar-Prompt-Knowledge-Eng-2024-04-08.pptxEIS-Webinar-Prompt-Knowledge-Eng-2024-04-08.pptx
EIS-Webinar-Prompt-Knowledge-Eng-2024-04-08.pptx
Earley Information Science
 
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Igalia
 
08448380779 Call Girls In Civil Lines Women Seeking Men
08448380779 Call Girls In Civil Lines Women Seeking Men08448380779 Call Girls In Civil Lines Women Seeking Men
08448380779 Call Girls In Civil Lines Women Seeking Men
Delhi Call girls
 
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdfThe Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
Enterprise Knowledge
 
Boost PC performance: How more available memory can improve productivity
Boost PC performance: How more available memory can improve productivityBoost PC performance: How more available memory can improve productivity
Boost PC performance: How more available memory can improve productivity
Principled Technologies
 
Mastering MySQL Database Architecture: Deep Dive into MySQL Shell and MySQL R...
Mastering MySQL Database Architecture: Deep Dive into MySQL Shell and MySQL R...Mastering MySQL Database Architecture: Deep Dive into MySQL Shell and MySQL R...
Mastering MySQL Database Architecture: Deep Dive into MySQL Shell and MySQL R...
Miguel Araújo
 

Recently uploaded (20)

A Call to Action for Generative AI in 2024
A Call to Action for Generative AI in 2024A Call to Action for Generative AI in 2024
A Call to Action for Generative AI in 2024
 
2024: Domino Containers - The Next Step. News from the Domino Container commu...
2024: Domino Containers - The Next Step. News from the Domino Container commu...2024: Domino Containers - The Next Step. News from the Domino Container commu...
2024: Domino Containers - The Next Step. News from the Domino Container commu...
 
IAC 2024 - IA Fast Track to Search Focused AI Solutions
IAC 2024 - IA Fast Track to Search Focused AI SolutionsIAC 2024 - IA Fast Track to Search Focused AI Solutions
IAC 2024 - IA Fast Track to Search Focused AI Solutions
 
Histor y of HAM Radio presentation slide
Histor y of HAM Radio presentation slideHistor y of HAM Radio presentation slide
Histor y of HAM Radio presentation slide
 
Advantages of Hiring UIUX Design Service Providers for Your Business
Advantages of Hiring UIUX Design Service Providers for Your BusinessAdvantages of Hiring UIUX Design Service Providers for Your Business
Advantages of Hiring UIUX Design Service Providers for Your Business
 
08448380779 Call Girls In Greater Kailash - I Women Seeking Men
08448380779 Call Girls In Greater Kailash - I Women Seeking Men08448380779 Call Girls In Greater Kailash - I Women Seeking Men
08448380779 Call Girls In Greater Kailash - I Women Seeking Men
 
A Domino Admins Adventures (Engage 2024)
A Domino Admins Adventures (Engage 2024)A Domino Admins Adventures (Engage 2024)
A Domino Admins Adventures (Engage 2024)
 
Handwritten Text Recognition for manuscripts and early printed texts
Handwritten Text Recognition for manuscripts and early printed textsHandwritten Text Recognition for manuscripts and early printed texts
Handwritten Text Recognition for manuscripts and early printed texts
 
Boost Fertility New Invention Ups Success Rates.pdf
Boost Fertility New Invention Ups Success Rates.pdfBoost Fertility New Invention Ups Success Rates.pdf
Boost Fertility New Invention Ups Success Rates.pdf
 
08448380779 Call Girls In Friends Colony Women Seeking Men
08448380779 Call Girls In Friends Colony Women Seeking Men08448380779 Call Girls In Friends Colony Women Seeking Men
08448380779 Call Girls In Friends Colony Women Seeking Men
 
Artificial Intelligence: Facts and Myths
Artificial Intelligence: Facts and MythsArtificial Intelligence: Facts and Myths
Artificial Intelligence: Facts and Myths
 
Factors to Consider When Choosing Accounts Payable Services Providers.pptx
Factors to Consider When Choosing Accounts Payable Services Providers.pptxFactors to Consider When Choosing Accounts Payable Services Providers.pptx
Factors to Consider When Choosing Accounts Payable Services Providers.pptx
 
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
 
EIS-Webinar-Prompt-Knowledge-Eng-2024-04-08.pptx
EIS-Webinar-Prompt-Knowledge-Eng-2024-04-08.pptxEIS-Webinar-Prompt-Knowledge-Eng-2024-04-08.pptx
EIS-Webinar-Prompt-Knowledge-Eng-2024-04-08.pptx
 
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
 
08448380779 Call Girls In Civil Lines Women Seeking Men
08448380779 Call Girls In Civil Lines Women Seeking Men08448380779 Call Girls In Civil Lines Women Seeking Men
08448380779 Call Girls In Civil Lines Women Seeking Men
 
What Are The Drone Anti-jamming Systems Technology?
What Are The Drone Anti-jamming Systems Technology?What Are The Drone Anti-jamming Systems Technology?
What Are The Drone Anti-jamming Systems Technology?
 
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdfThe Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
 
Boost PC performance: How more available memory can improve productivity
Boost PC performance: How more available memory can improve productivityBoost PC performance: How more available memory can improve productivity
Boost PC performance: How more available memory can improve productivity
 
Mastering MySQL Database Architecture: Deep Dive into MySQL Shell and MySQL R...
Mastering MySQL Database Architecture: Deep Dive into MySQL Shell and MySQL R...Mastering MySQL Database Architecture: Deep Dive into MySQL Shell and MySQL R...
Mastering MySQL Database Architecture: Deep Dive into MySQL Shell and MySQL R...
 

Detection of transgenic canola (Roundup Ready® - Monsanto)

  • 1. Claudio Iannetta (s3154021) Supervisor: Saman Buddhadasa Senior Food Scientist Food Metrology National Measurement Institute
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.
  • 19.
  • 20. Project – Flow Chart ✔ ✔ ✔
  • 21.
  • 22.
  • 23.
  • 24.
  • 25.
  • 26.
  • 27.
  • 28.
  • 29.
  • 30.
  • 31.
  • 32.
  • 33.
  • 34.
  • 35.
  • 36.
  • 37.
  • 38.
  • 39. Data Analysis The consistency of the results is assured if Coefficient of Variation is below 25%, which is the recommended value from EU Network of GMO Laboratories Sample A Sample B E ( FatA ) R 2 ( FatA ) 1 2.18 x 10 3 917.891 95% 99% 2 2.26 x 10 3 840.949 3 2.24 x 10 3 879.143 Mean 2.23 x 10 3 879.327 SD 41.63 38.47 CV 1.87% 4.37%
  • 40.

Editor's Notes

  1. Self introduction: Good morning/afternoon to everybody My name is Claudio Iannetta and I am a Master student in Biotechnology, with a major in Food Sc &amp; Tech here at RMIT University My research project has been a collaboration between RMIT-University and NMI (National Measurement Institute), both placed in Melbourne. The supervision of the project has been guided by Dr. Saman Buddhadasa (NMI) and Dr Gregory Nugent (RMIT University).