2. EXC Component

3. Globular regions with specific binding site for the RGD sequences on fibronectin or specific binding sites for specific amino acids on laminin

4. Alpha and beta subunit link to alpha helix and the two cytoplasmic domains interact with actin binding proteins that help integrins assemble actin filaments

5. Integrins doent directly link to actin

6. They Indirectly bind to actin by binding to actin binding proteins

7. Intracellular component

8. Binding site for talin

9. Transmembrane component

10. Attach cell to basal lamina

11. Combinations of integrins allows for differential signaling in that enironment

12. Different cells express different combinations of integrins

13. Different Cells have Different Expressions/Interactions of integrins

14. A5B1

15. Allows cells to interact with fibronectin

16. Prime component of fibrillar macromolecules in the EXC Matrix below basal lamina

17. A6B1

18. Allows cells to interact with laminin

19. Laminin is the basal lamina

20. A7B1

21. Muscle cells

22. LFA

23. White blood cells/immune cells of the blood

24. Need to be able to move through endothelial cells, enter into tissue, bind to laminin and deal with infection at that site

25. A6B4

26. Allows epithelial cells to interact with basal lamina

27. Can be targeted in disease states that result in blistering of skins b/c epithelial cells don’t attach well to basal lamina

28. Mutations affect cells

29. Beta1

30. Embryo can’t implant because of the role of allowing embryo to develop

31. Alpha7

32. Different types of muscular dystrophy

33. Beta2 or Alpha subunit of LFA

34. WBC can’t migrate into tissue to deal with infections

35. Leukocyte Adhesion Deficiencies

36. Neutrophiles are the first WBC to move to infection, primary response to infection

37. If no migration, infection has an increased chance of infecting host cells

38. Focal Adhesions

39. Assembled actin filaments and actin associated proteins and integrins

40. A site where actin filaments assemble due to integrin receptors linked to actin binding proteins

41. Focal adhesions are site that allow cell to adhere to EXC matrix

42. Kinases that activate aseembly of focal adhesions

43. Focal Adhesion Kinase (FAK)

44. Turned on after integrin activation

45. Drives deoplymerization actin and breakdown of focal adhesion

46. In order for cells to be mobile, FA must first form but then be broken down. b/c if maintainted, the cell can’t move and stuck at that spot. Say break one at back and make one at front for movement

47. Fibroblasts/immune cells/RBC and other mobile cells

48. Integrin Receptor Kinase

49. Help drive signaling at site of integrin acticvation and regulate what happens with actin filaments at that site

50. Talin interacts with cytoplasmic regions of integrin receptors when bound to fibronectin (activated) or laminin

51. Confo change occurs as integrins receptors are bound by ligand in EXC matrix

52. Allows to interact with actin associate proteins like talin

53. Alpha-Actinin

54. Bind to growing actin filaments to organize them

55. Generates parallel association as actin filaments begin to form

56. Actin associated molecules are linked to signaling pathways like Ras, driving rate of assembly of actin filaments

57. Hemidesmosomes

58. Immobile cells

59. Exists at basal membrane of epithelial cells (polarized, apical side where microvilli are, and basal side, near basal lamina)

60. Basal lamina has embedded in it, fibrillar types of EXC matrix proteins, one being laminin

61. Transmembrane integrin proteins links epithelial cell to proteins of EXC matrix

62. A6B4 integrin is specifically found in hemisdesmosomes of epithelial cells

63. Links with intermediate filaments

64. This association organizes intermediate filaments in epithelial cells to help with tensile functions/mechanical stress functions of intermediate filaments

65. Link to plectin proteins that associate with integrins, allowing them to link with intermediate filaments

66. Plaque

67. Made of plectin proteins

68. Link with integrins

69. BPAG

70. Second type of transmembrane protein that binds to EXC matrix

71. Doesn’t link to actin