1. Cornell
MSU Susan Brown
Amy Iezzoni (PD) Kenong Xu
Jim Hancock
Dechun Wang Clemson
Cholani Weebadde Ksenija Gasic
Gregory Reighard
Univ. of Arkansas
John Clark Texas A&M
WSU USDA-ARS
Cameron Peace Dave Byrne
Nahla Bassil
Dorrie Main Gennaro Fazio Univ. of CA-Davis
Kate Evans Univ. of Minnesota
Jim Luby Chad Finn Tom Gradziel
Karina Gallardo
Raymond Jussaume Chengyan Yue Plant Research Intl, Carlos Crisosto
Vicki McCracken Netherlands
Nnadozie Oraguzie Oregon State Univ. Eric van de Weg Univ. of New Hamp.
Mykel Taylor Alexandra Stone Marco Bink Tom Davis

2. International Collaborators
Plant Research International, Netherlands
East Malling Research, UK Andres Bello University, Chile
CRA-FRU, Rome, Italy Plant & Food Research, New Zealand
IASMA, Trento, Italy ARC, South Africa
INRA (Bordeaux, Avignon, Angers), France
RosBREED
participant
location
+ 2 Scientific Advisory Panel members (Spain, New Zealand)

3. Bridging the Chasm!

4. The RosBREED Approach
Socio-economics information
DNA information

5. RosBREED’s “Work Packages” (5 Objs)
1) Enhance new cultivar adoption, enlarge market potential,
increase consumption
= socio-economics knowledge of trait values
2) Establish sustainable infrastructure for MAB in Rosaceae
= genome scanning capability, new QTLs (“jewels”)
3) Integrate socio-econ and genomics resources into breeding
= pedigree-based breeding information management system
4) Conduct MAB in demo breeding programs, fruit quality focus
= MAB implementation
5) Enhance sustainability of cultivar development
= tech transfer to more breeders & engage other stakeholders

6. “Jewels in the Genome”
- discovering, polishing, applying
QTL discovery MAB Pipelining Breeding
(looks promising...) (polishing...) (assembling into
masterpieces)

7. SNP Genome Scans
Illumina Infinium® arrays:
Peach: 9K Apple: 8K Cherry: 6K
Genotype obtained for
screened sweet cherry
germplasm
SNP failed

8. Reference Germplasm
Minnesota
BP Set
(n=160)
CROP
Washington Any breeder’s
BREEDING
BP Set REFERENCE PEDIGREE
additional
(n=160) SET SETS
data
n=480
New York
BP Set
(n=160)

9. Jewel Discovery
SNP
genome scans
PBA
Standardized
phenotypic data

10. Socio-Economics Surveys (example for apple)
Washington Michigan Market
Breeders
Producers Producers Intermediaries
Fruit flavor 43 41 23
Fruit crispness 15 23 10
Exterior color 26
Fruit firmness 6 7 5
Shelf life at retail 7 7 3
Sweetness/soluble solids 6 7 3
Sugar/acid balance 9 7
External appearance 13
No storage disorders 7 4
Disease resistance 2 5
Storage life 5
Other fruit quality attributes 2 3
Size 3
Juiciness 2
Tartness
Shape
Phytonutrient
Aroma
% of respondents 0 20 40 60 80 100

11. Pedigree-Based Breeding Information
Management System for MAB

12. Databasing RosBREED Information:
The GDR
uploading data
downloading data
analyses
reports/descriptions

13. MAB Successes So Far
Two Jewels in routine use
New generations enriched
WSU apple & sweet
for favourable alleles
cherry breeding programs
est. $80K saved from 2011
cherry seedling culling alone!

14. Future Funding...
2009
2010
2011
-crops?
2012
2013
-traits?
2014 -approaches?
2015
2016
-resources?
2017 -partners?
2018
2019
2020
2021
2022

15. Cornell
MSU Susan Brown
Amy Iezzoni (PD) Kenong Xu
Jim Hancock
Dechun Wang Clemson
Cholani Weebadde Ksenija Gasic
Gregory Reighard
Univ. of Arkansas
John Clark Texas A&M
WSU USDA-ARS
Cameron Peace Dave Byrne
Nahla Bassil
Dorrie Main Gennaro Fazio Univ. of CA-Davis
Kate Evans Univ. of Minnesota
Jim Luby Chad Finn Tom Gradziel
Karina Gallardo
Raymond Jussaume Chengyan Yue Plant Research Intl, Carlos Crisosto
Vicki McCracken Netherlands
Nnadozie Oraguzie Oregon State Univ. Eric van de Weg Univ. of New Hamp.
Mykel Taylor Alexandra Stone Marco Bink Tom Davis

16. Cross Assist
Find it at:
www.rosaceae.org/bt_cross_planning_tool/ca_front_page
or simply on the GDR under “Breeders Toolbox”