6. Frame 1 @ 0.33sec Frame 2 @ 0.67sec Frame 3 @ 1.00sec Frame 4 @ 1.33sec Frame 5 @ 1.67sec Frame 6 @ 2.00sec Frame 7 @ 2.33sec Frame 8 @ 2.67sec Frame 9 @ 3.00sec Frame 10 @ 3.33sec Frame | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Lambda Scan from 550-610nm: Image Series above correlate to each wavelength/intensity reading in chart/graph to right Structure is fibroblast actin labeled with Alexa Fluor 488: weaker, post-peak EM signal
7. Mark and Find Function: 3 random x,y,z coordinates identified & recaptured later Top row series: manual images before defining xyz positions with “Mark” function Bottom row series: Auto images captured with “Find” function by recalled xyz positions
8. Two frames from Movie of 3D volume rendering of z-stack: rotating at -45deg y axis viewing angle Image 1: Frame 9 of 20 @ 2.5 sec of 5.0 sec Image 2: Frame 20 of 20 @ 5.0 sec of 5.0 sec
9. Movie of 3D volume rendering of z-stack, rotating at - 45deg. y axis viewing angle
10. Original Separation 1 Separation 2 Dye Separation Series: 3 channels, Auto- separate, weak strength Original
11. Muntjac fibroblast slide display signal in all 3 channels at 40x oil, test image (unmounted slide to reboot & subsequently could not locate this area of slide: all nuclei were bleached elsewhere