Fred Martin Micrograph Portfolio

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Sample of images taken for MMP program using Zeiss Axioimager, Nikon, Olympus AX41, Leica

Educação Negócios Tecnologia

PROJECT PORTFOLIO FRED MARTIN 12/08/2009 Dr. Giorgi BIOSC 2

Invt4_10x_CompositeRGB_002.jpg 20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095 SPECIMEN (A1) NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE

invt4_a1_Composite_RG_DapiLUT_75.jpg 20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (A2) NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE

FluoCells prepared slide #2: BPAE bovine pulmonary artery endothelial cells Image A - 20091105: Zeiss #1 20x ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS

FluoCells ® prepared slide #1: BPAEC, Zeiss 63x 20091016_INVG1_AREA3_CompositeAdj_jpg.jpg ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS

NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS FM_INVT1_A1_CompositeAdjRGB_xDIC BPAE CELLS INVITROGEN #1

20x_LilyAnther_Fluro_RGB.jpg 20091123 /Nikon2 /20x ; R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (B) NIKON, FLUORESENCE

FM_lycopodiumCaptured_FluoroRGBnDIC_20x_2.jpg 20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (C) NIKON, FLUORESENCE + DIC

SPECIMEN (F) Typha_10x_Composite_001_RGB_Adj 20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095 COMPOSITE CHANNEL: RED CHANNEL: GREEN CHANNEL: BLUE NIKON, FLUORESCENSE (3 CHANNELS + OVERLAY)

Pollen Grain: Abutilon x hybridium @ 20x ,[object Object],EM Channel: Red Exp: 96.7ms Gain:7.1 Offset: -876 EM Channel: (BF) Exp: 16.7ms Gain: 4.5 Offset: -161 Composite OLYMPUS, OWN SPECIMEN, FLUORO OVERLAY + 3 CHANNELS

fm__pseudocolor_lycopod_20x_CompositeRGBnDIC.jpg SPECIMEN (D) 20091207 /Nikon2 /20x ; “R”: 13ms/ 159/ 0.51/ 2,830 “G”: 6.6ms/ 159/ 0.51/ 2,830 “B”: 12.0ms/ 159/ 0.51/ 2,830 NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)

Multichannel _Shrimp_Pseudocolor2_RGB.jpg SPECIMEN (E) 20091118 /Nikon4 /20x ; R: --ms/ --/ --/ -- G: --ms/ --/ --/ -- --B: ms/ --/ --/ -- Note: metadata not logged NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)

$Cilia PSCCE Cells Basal Lamina Pseudo Stratified Ciliated Columnar Epithelial Cross section: area 3 image Cilia Basal Lamina Nucleus Cilia (Apparent Stratification) Exp. 500.7ms Hist: 79/252 Gamma: 0.99 Auto_exp: OFF Gain: 3.1x 2009.10.01 Leica #2 60x (dry) Nucleolus { } LEICA, BRIGHTFIELD$

PSCCE Cells Cell Membrane Cilia (Apparent Stratification) Nuclei Basal Lamina LEICA, BRIGHTFIELD 48 BitDepth: 20 / 255 Histogram: On Auto_Exp? 0.97 Gamma: 2009-10-07 DateImage: 53 % Brightness: 2.2 x Gain: 1.0 s Exposure:

ZEISS, DIC FM_INVT4_A1_DIC-0005.JPG 20091209 /ZEISS 1 /20x ; DIC : EXP 69ms/ CT -0.50 / GAMMA 1.0 / BRT 1.0

[object Object],[object Object],[object Object],[object Object],[object Object],RESULTS: DIGITAL COUNT DETAIL – FIELD OF VIEW A: Onion Root Tip Meta Data Scope: Olympus 3 (A) 10x, rheo: full Iris ~ 30% 1.1 ms 2.8 gain -1,860 offset Note: Manual count done at 20x on Olympus3 OLYMPUS, MORPHOMETRY

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Fred Martin Micrograph Portfolio

1. PROJECT PORTFOLIO FRED MARTIN 12/08/2009 Dr. Giorgi BIOSC 2

2. Invt4_10x_CompositeRGB_002.jpg 20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095 SPECIMEN (A1) NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE

3. invt4_a1_Composite_RG_DapiLUT_75.jpg 20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (A2) NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: TISSUE

4. FluoCells prepared slide #2: BPAE bovine pulmonary artery endothelial cells Image A - 20091105: Zeiss #1 20x ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS

5. FluoCells ® prepared slide #1: BPAEC, Zeiss 63x 20091016_INVG1_AREA3_CompositeAdj_jpg.jpg ZEISS, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS

6. NIKON, FLUORESENCE, MOLECULAR PROBES SLIDE: CELLS FM_INVT1_A1_CompositeAdjRGB_xDIC BPAE CELLS INVITROGEN #1

7. 20x_LilyAnther_Fluro_RGB.jpg 20091123 /Nikon2 /20x ; R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (B) NIKON, FLUORESENCE

8. FM_lycopodiumCaptured_FluoroRGBnDIC_20x_2.jpg 20091123 /Nikon2 /20x ; DIC: 20ms/ 79/ 0.94/ 3,310 R: 47ms/ 79/ 0.94/ 3,310 G: 41ms/ 95/ 0.67/3,054 B:392ms/159/0.51/2,830 SPECIMEN (C) NIKON, FLUORESENCE + DIC

9. SPECIMEN (F) Typha_10x_Composite_001_RGB_Adj 20091207 /Nikon2 /10x ; DIC: 73ms/ 1,503/ 0.95/ 4,095 R: 97ms/ 79/ 0.63/ 4,095 G: 200ms/ 239/ 0.95/4,095 B:500ms/223/0.77/4,095 COMPOSITE CHANNEL: RED CHANNEL: GREEN CHANNEL: BLUE NIKON, FLUORESCENSE (3 CHANNELS + OVERLAY)

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11. fm__pseudocolor_lycopod_20x_CompositeRGBnDIC.jpg SPECIMEN (D) 20091207 /Nikon2 /20x ; “R”: 13ms/ 159/ 0.51/ 2,830 “G”: 6.6ms/ 159/ 0.51/ 2,830 “B”: 12.0ms/ 159/ 0.51/ 2,830 NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)

12. Multichannel _Shrimp_Pseudocolor2_RGB.jpg SPECIMEN (E) 20091118 /Nikon4 /20x ; R: --ms/ --/ --/ -- G: --ms/ --/ --/ -- --B: ms/ --/ --/ -- Note: metadata not logged NIKON, BRIGHTFIELD (3 CHANNEL PSEUDO-COLORIZATION)

13. Cilia PSCCE Cells Basal Lamina Pseudo Stratified Ciliated Columnar Epithelial Cross section: area 3 image Cilia Basal Lamina Nucleus Cilia (Apparent Stratification) Exp. 500.7ms Hist: 79/252 Gamma: 0.99 Auto_exp: OFF Gain: 3.1x 2009.10.01 Leica #2 60x (dry) Nucleolus { } LEICA, BRIGHTFIELD

14. PSCCE Cells Cell Membrane Cilia (Apparent Stratification) Nuclei Basal Lamina LEICA, BRIGHTFIELD 48 BitDepth: 20 / 255 Histogram: On Auto_Exp? 0.97 Gamma: 2009-10-07 DateImage: 53 % Brightness: 2.2 x Gain: 1.0 s Exposure:

15. ZEISS, DIC FM_INVT4_A1_DIC-0005.JPG 20091209 /ZEISS 1 /20x ; DIC : EXP 69ms/ CT -0.50 / GAMMA 1.0 / BRT 1.0

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Fred Martin Micrograph Portfolio

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