MSD Finca Productiva Reproduccion

1. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s
a n d fi e l d t r i a l data
Estrumate Prostaglandin
Consistently Outperforms Lutalyse ®
Summary of Eight Studies

2. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Estrumate Prostaglandin Consistently
Outperforms Lutalyse Summary Of Eight Studies ®
• Half-life for EstrumatE prostaglandin is three hours compared to a
few minutes for Lutalyse. EstrumatE prostaglandin lasts much longer 1
than Lutalyse. the sensitivity of the corpus luteum to prostaglandin
injection increases progressively during the estrous cycle. 2
• EstrumatE prostaglandin is a more potent luteolytic agent than Lutalyse. 3
• EstrumatE prostaglandin quickly reduces progesterone levels and provides 4
a rapid estrus response. 5
• EstrumatE prostaglandin features a convenient 2-mL dose for heifers and cows.
a 2-mL dose is easier to use and gives you confidence in EstrumatE prostaglandin’s
performance.

3. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Seguin’s Work 5
Dairy
• Cows were treated on Day 8 of the estrous cycle.
• Compared estrus response and conception rate by product.
• Evaluated estrus response until Day 6 after injection.
• Cows were artificially inseminated 12 hours after estrus was detected.

4. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Seguin’s Work 5
(continued)
Dairy
r e sult s, e s t r ous r e sp onse by p r oduc t r e s u lt s , p e r c e n t a nim a l s in h e at
Time to Estrus Estrumate ®
Lutalyse ®
Estrumate ®
Lutalyse ®
(n=62) (n=62)
48 hours* 37 27
48 hours post injection* 59.7% 43.5%
60 hours 17 21
60 hours post injection 87.0% 77.0%
72 hours 3 5
84 hours post injection 92.0% 85.0%
84 hours 0 0
156 hours post injection 96.8% 88.7%
96 hours 0 1
108 hours 1 0 r e s u lt s , c on c e p t ion r at e b y p r o du c t
120 hours 1 0
Estrumate ®
Lutalyse ®
132 hours 0 0 (n=62) (n=62)
144 hours 1 1 Conception rate 62.5% 56.3%
156 hours 0 0
total responses 60/62 55/62
Response rate 96.8% 88.7%
*p=0.07

5. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Martineau’s Work 3
Dairy
s t u dy c on du c t e d in c a n a di a n
d a ir y op e r at ion s f r o m
m ay 1, 19 9 3 t o ju n e 1, 19 9 4
• Lactating dairy cows and heifers having
a corpus luteum determined by rectal
palpation were randomly assigned to
treatment groups.
Estrumate ®
Lutalyse ®
Number cases 101 102
Conception rate 41.8% 33.7%
Pregnancy rate 34.9% 29.3%
*Differences between treatment
groups not statistically significant.

6. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
New York Dairy Field Trial 6
Dairy
• Compared reproductive performance of • all cows were bred the following
animals receiving Estrumate vs. Lutalyse.
® ®
thursday between noon and 2:00 p.m.
• Ovsynch protocol utilized. • Cows were pregnancy checked by
the dairy’s consulting veterinarian
• trial began November 2003 and was
at day 32-46 after breeding.
completed september 2004.
b r e e din g p r o gr a m r e s u lt s *
• the first tuesday after 45 days in milk, an Estrumate Lutalyse
® ®
injection of prostaglandin, based on the Number cows/treatment 232 222
animals’ treatment group, was administered. First service conception rate 39% 34%
• the following week on tuesday, the second service conception rate 43% 32%
cows all received a Gnrh injection.
• the following tuesday the cows received
an injection of prostaglandin, based *Differences between treatment
groups not statistically significant.
on the animals’ treatment group.

7. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
California Dairy Field Trial 6
Dairy
• Compared reproductive performance of e c ono mic s
animals receiving Estrumate vs. Lutalyse.
® ®
• For every 1% increase in pregnancy rate,
• trial began January 2004 and was per cow profitability increases up to $17.00.
completed November 2005.
• Calculate the economic advantage
• Cows received prostaglandin of Estrumate in your operation.
at 50-100 days in milk.
• Cows were pregnancy checked by
the dairy’s consulting veterinarian
at day 35-49 after breeding.
r e s u lt s *
Estrumate ®
Lutalyse ®
Number cows/treatment 1,555 1,491 *Differences between treatment
First service conception rate 30.4% 29.9% groups not statistically significant.

8. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Idaho Dairy Field Trial 6
Dairy
• Compared reproductive performance of summary
animals receiving EstrumatE vs. Lutalyse. ®
Continued trends of superior EstrumatE
• Injected products and bred performance in conception rates
following an observed heat.
two injections: 35.0% vs. 32.0%
• study conducted in the fall/winter 2004.
three injections: 30.0% vs. 28.0%
r e s u lt s *
2 Injections 3 Injections
Conception rate Conception rate
35.0% 30.0%
Estrumate
(39/111) (39/131)
32.0% 28.0%
Lutalyse
(38/119) (39/139) *Differences between treatment
groups not statistically significant.

9. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Canadian Work 7
Beef
One injection to beef heifers, where CL was • Beef heifers were palpated for cyclicity
palpated before injection* and randomly assigned to one of
two prostaglandin treatments.
Estrumate ®
Lutalyse ®
• two injections were given 11 days apart
(n=56) (n=63)
and heifers bred on estrus detection,
First service Conception rate 61.0% 57.0% following each prostaglandin injection.
Pregnancy rate 96.0% 87.0% • Heat detection and aI continued after
synchronization for approximately
one month.
two injections given 11 days apart to cycling
beef heifers* • at that time, red angus bulls were used
for the remainder of the breeding season.
Estrumate ®
Lutalyse ®
• Cows were artificially inseminated 12 hours
(n=129) (n=126) after estrus was detected.
Estrus 92.0% 86.0%
Pregnancy rate 91.0% 83.0% *Differences between treatment
groups not statistically significant.

10. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Oregon Beef Herd Field Trial 6
Beef
• Compared reproductive performance of
animals receiving Estrumate vs. Lutalyse.
® ®
• two injections 11 days apart, with
breeding 12 hours after estrus detected.
• study conducted in summer 2004.
r e s u lt s
Estrumate ®
Lutalyse ®
P Value
Heifer 39.6% 35.0%
0.2009
Conception rate (143/361) (130/371)

11. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Florida Beef Herd Study 8,9
Beef
• Evaluated pregnancy rates to a timed aI results, conception r ates by products*
program in Bos indicus x Bos taurus cows.
EstrumatE: 41% (n=297)
• used Gnrh, PG F2a and mGa.
Lutalyse: 36% (n=297)
• Cosynch hybrid program
Day 0: Gnrh
Day 1-7: mGa, 0.5 mg/head/day
Day 7: EstrumatE or Lutalyse ®
Day 10: timed aI plus Gnrh
*Differences between treatment
groups not statistically significant.

12. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Stage of Cycle Effects on Response to
Different GnRH + Prostaglandin F2a Treatments 6
m at e r i a l s a n d m e t ho d s • three different PGF protocols
injected seven days after GnrH.
• Presynchronization with a two-dose
modified protocol. – Lutalyse 25 mg
– Lutalyse 12.5 mg (day 7 and 8)
– Lutalyse 25 mg on D -14
®
– EstrumatE 0.5 mg
– Lutalyse 12.5 mg on D -3 and D -2
• Blood samples collected before
• Estrus detected with HeatWatch.
injection and at every 48 hours until
• GnrH injected in four different estrous response and/or ovulation.
days of the estrous cycle. • ultrasound imaging.
– Days 2, 5, 12, 18

13. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Stage of Cycle Effects on Response to
Different GnRH + Prostaglandin F2a Treatments 6
Estrous response (%), CL regression (%) and Interval to Estrus
of Different PGF Products Injected 7 Days after GnrH Injection
110% 95 Estrous response 5 days
after PGF injection
100% 90
90% 90 Percentage of cows
95%
Interval to Estrus (h)
with CL regression
80%
81% Interval (hours) from PGF
70% 85
% Cows
74% injection to onset of estrus
60%
63%
50% 77 80
40% *Differences between treatment
30% 75 groups not statistically significant.
20%
10% 70
Lutalyse 25 mg
®
Estrumate ®

14. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
Why Estrumate Is Superior to Lutalyse
• Cloprostenol sodium, the active
Estrumate *®
Lutalyse **
®
ingredient in EstrumatE, has a longer
half-life than dinoprost tromethamine, Half-life 3 hours
Extremely short
(few minutes)
the active ingredient in Lutalyse. ®
almost completely
• Cloprostenol is a more potent Not degraded by 15-
cleared after one or
Clearance hydroxy-dehydrogenase
luteolytic agent than dinoprost. and 13,14-reductase
two passages through
the liver and/or lungs
• Cloprostenol is not degraded by
15-hydroxydehydrogenase
and 13,14-reductase.
* EmEa, the European agency for the Evaluation of
medicinal Products. Committee for Veterinary medicinal
Products. Cloprostenol and r-Cloprostenol summary
report, april 1997.
** EmEa, the European agency for the Evaluation of
medicinal Products. Committee for Veterinary medicinal
Products. Dinoprost, summary report, June 1997.

15. e s t r um at e ® fa c t s , p ub l i s he d s t udi e s a n d f i e l d t r i a l dat a
References
EmEa, the European agency for the Evaluation of medicinal Products. “Cloprostenol and r-
1
Cloprostenol.” summary report, april 1997.
EmEa, the European agency for the Evaluation of medicinal Products. “Dinoprost.” summary report,
1
June 1997.
2
Berardinelli, J.G., et al. "Effect of prostaglandin dosage and stage of estrous cycle on the estrous
response and corpus luteum function in beef heifers.” Theriogenology, 32: 301-314, 1989.
3
martineau, r. “Dinoprost Versus Cloprostenol: Does route of Injection modulate their Efficacy in Dairy
Cattle?” The Bovine Practitioner, pp. 10-19, February 2003.
4
Hayashi, K., et al. "Changes in Prostaglandin secretion by the regressing Bovine Corp Luteum.” Elsevier
– Prostaglandins & Other Lipid mediators. 70:339-349, 2003.
5
seguin, B., et al. “Cloprostenol and Dinoprost tromenthamine in Experimental and Field trials treating
unobserved Estrus in Dairy Cows.” The Bovine Practitioner, pp. 85-90, November 1985.
6
Data on file at schering-Plough animal Health Corporation.
Comparison of Estrumate and Lutalyse in Beef Heifers. Joint Federal Provincial
7
Demonstration Project at Douglas, manitoba, Canada 1981-1983.
8
Hiers, Ea. 2003 Florida Beef report, pp 77-79.
9
Hiers, Ea et al. Journal of Animal Science 81:830-835, 2003.
Copyright © 2006, schering-Plough animal Health Corporation. all rights reserved. EstrumatE is a trademark of
schering-Plough animal Health Corporation. LutaLysE is a registered trademark of Pfizer animal Health. sPaH-Est-48a