5. Niacin test and nitrate reduction test -ve Niacin test &Nitrate reduction test &PO+ve no effect 0.5%glycerol-improves growth Dysgonic-sparsely Eugonic-grows luxuriantly microaerophilic primary isolation,aerobic on subculture obligate aerobe M.bovis M.tuberculosis
6. In liquid media-growth begins at bottom,creeps up the sides,forms prominent surface pellicle Flat smooth,white,moist,breakup easily when touched Solid medium-dry,rough,raised,irregular,wrinkled colonies-creamy white to yelow on incubation inc growth Sodium pyruvate-inc growth
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17. ZN smear evln. & AFB report 4 + 1 F >= 10 3 + 1 F 1-9 2 + 10 F 1-9 1 + 100 F 1-9 Repeat smear 300 F 1-2 AFB not seen 300 F 0 report OIL imm.field No. of AFB
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