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Fundamentals of Biology
4.1 The Ingredients of Life
• A. The Building Blocks
• 1 Organic compounds – molecules containing
  C, H, & O
• Make life possible
• High-energy molecules
 Energy used to synthesize
 Energy released in breakdown
• Four types of organic compounds
2 Carbohydrates – sugars

• Glucose – metabolized for energy
• Starches – long chains of simple sugars used
  for energy storage
• Chitin – skeletal material
• Cellulose – cell structure
3 Proteins
•   Chains of amino acids
•   Muscles are mostly made up of proteins
•   Enzymes – catalyze reactions
•   Structural proteins – skin, hair, skeleton
•   Hormones
4 Lipids
• Fats, oils, & waxes
• Energy storage – more than twice as much as
  sugar
• Water repellant
• Buoyancy
• Insulation
• Hormones
5 Nucleic Acids
• Store and transmit the genetic information of
  all living things
• Long chains of subunits called nucleotides
• DNA – instructions for the construction and
  maintenance of an organism; the complete set
  is called the genome
• The nitrogen bases are sequenced into genes
  that code for a specific protein
• RNA – helps DNA
B. The Fuel of Life
• ATP – the molecule used to store energy; like
  a rechargeable battery
• You use ~ 125 lbs./day
• Organisms need to capture, store and use
  energy
• Most organisms use only two sets of reactions
1 Photosynthesis: Making the Fuel
• Algae, plants, and some microorganisms
• Capture the sun’s energy and use it to make
  glucose
 The pigment chlorophyll captures the solar
  energy
 CO2 + H2O → C6H12O6 (glucose) + O2
• We rely on photosynthesis for food and oxygen
• Organisms that photosynthesize are called
  autotrophs
 Plants on land; bacteria and algae in the ocean
2 Respiration: Burning the Fuel
• Both autotrophs and heterotrophs do it
• Releases the energy from org. compounds
• Reverse of photosynthesis
 Organic matter (glucose) + O2→ H2O + CO2
• Similar to burning wood or oil
• Chemical energy captured in ATP
• Aerobic – uses oxygen, more efficient
• Anaerobic – does not use oxygen, less
  efficient
3 Primary Production
• Most of the glucose is used for fuel or
  converted into other types of org. compounds
• The organic matter autotrophs make is called
  primary production
 Used by the organism for growth and
  reproduction
• Autotrophs are also called producers
4 The Importance of Nutrients
• Vitamins, minerals and other substances are
  needed to convert glucose into other org.
  compounds
• Nitrogen for proteins & nucleic acids
• Phosphorus for nucleic acids
• Silica (SiO2) to make shells
• Iron – necessary, but a limited resource in the
  ocean
4.2 Living Machinery

• Organic compounds are organized into
  functional units that are alive
A. Cells and Organelles
      Cell – basic unit of life
 All organisms are made of cells
 Wrapped in a cell membrane
• Cell is filled with jelly-like cytoplasm
• Organelles have specific jobs in the cell
1. Structurally Simple Cells: Prokaryotes

 Prokaryotes are primitive cells
 Ancient, simple, small
 No membrane-bound organelles
 Bacteria
 Prokaryotes have few structures:
 Cell wall – support
 Ribosomes – assemble proteins
 DNA – loose in the cytoplasm
 Flagella – locomotion
2. Structurally Complex Cells: Eukaryotes
  Eukaryotic cells are organized and complex
 Larger than prokaryotes
  Have specialized organelles:
 Nucleus – contains chromosomes (DNA)
 Endoplasmic reticulum – make proteins and other org.
  molecules for the cell
 Golgi apparatus – package and transport molecules
 Mitochondria – respiration center to provide energy
 Flagella and cilia – for movement
  Only in plant & algal cells
 Chloroplasts – photosynthesis center
 Cell wall - support
B. Levels of Organization
1. A cell is self-contained and can carry out all the functions
   necessary for life
• Unicellular – all prokaryotes and some eukaryotes
• Multicellular – most eukaryotes
 Human body has 100,000,000,000,000 cells
• In multicellular organisms cells specialize to perform
   different tasks for the organism
• Cells that act together for a specific job are called tissues
 Muscle, nervous, bone, blood, epithelial
• Tissues are organized into organs to carry out specific
   functions
 Liver, kidney, heart, skin, brain
• Organs act together in an organ system
 Skeletal, muscular, excretory, endocrine, digestive
2. Organization exists outside the individual
                  organism
 Species – one type of organism
 Blue mussel
 Population – a group of one species
 A bunch of blue mussels
 Community – several different populations that live
 and interact in an area
 Blue mussels, crabs, barnacles, & chitons living on a
 rock
 Ecosystem – the communities living together with the
 physical environment
 Living on a rocky shore with seawater, air,
 temperature, sunlight, etc.
4.3 Challenges of Life in the Sea

• Marine organisms must cope with different
  problems than on land
• They have evolved ways to adapt to their
  marine habitat
• Most important is maintaining homeostasis
Keeping their internal condition normal
  regardless of the external condition
A. Salinity

• Marine organisms are immersed in a medium
  – sea water – that can greatly affect their cell
  function
• Enzymes and organic molecules are sensitive
  to ion concentration (salinity)
1. Diffusion and Osmosis
• Dissolved ions move around in water
• Random movement spreads them out in an even
  distribution
• Results in diffusion – movement from high low
  concentration
• When concentrations are different inside and
  outside a cell, substances will move in/out by
  diffusion
 Salt from seawater will diffuse into the cell
 Nutrients will diffuse out of the cell
• The cell membrane blocks block diffusion
 It’s selectively permeable – it allows only some
  substances to go in/out
• Water is a small molecule and can fit through the
  cell membrane
 It also diffuses from high → low concentration
 If a cell has more solutes inside than outside,
  water will stream in and swell the cell
 If the seawater has more salt, water will leave
  and the cell will shrivel
 This diffusion of water is called osmosis
• Cells may need to move materials against
  diffusion (low high)
 e.g. expelling extra salt or taking in more sugar
 Active transport – proteins in the cell membrane
  pump materials using ATP
1/3 of the cell’s energy is spent on this
2. Regulation of Salt and Water Balance
• Marine organisms have adapted ways to balance water
  and salt
• Osmoconformers –their internal concentrations
  change with the salinity of the seawater
 Live in a narrow range of salinity
• Osmoregulators – control internal concentrations to
  avoid osmotic problems
 Can tolerate changes in salinity better
 Can change their internal concentrations to match the
  seawater
 Salt water fishes lose water by osmosis
     Drink water or reduce urine amount to replace lost
  water
     Excrete excess salts in the urine or through the gills
 Freshwater fishes gain water by osmosis
Don’t drink water or produce lots of urine
Salt absorbed by gills
 Some marine birds and reptiles have special
 glands to get rid of excess salt
 Most algae have rigid cell walls that resist the
 swelling caused by osmotic water gain
B. Temperature
 Metabolic reactions speed up/slow down
 when temperature goes up/down
 Metabolic rate doubles every 10oC
 At extreme temps most enzymes cease to
 function
 Marine organisms are adapted to live in a
 temp range
 Thus determining what regions of the oceans
 they live
 Ectotherms – “cold blooded” lose their heat to the
  seawater
 Endotherms – “warm blooded” retain heat and keep
  their body temp higher than the water
 Mammals, birds, and some large fishes
 Poikilotherms – body temp changes with the temp of
  the seawater
 Incl. all ectotherms & endothermic fishes
 Homeotherms – keep internal temp the same,
  regardless of outside temp
 Produce more heat as need to keep their metabolic
  activity high
 Mammals & birds
 They need to eat more food
 Insulate their bodies with feathers, hair, and blubber
C. Surface-to-Volume Ratio
 Heat and materials exchange across the
 surface of an organism
 The surface-to-volume ratio (S/V ratio)
 determines how rapidly this happens
 As organisms get larger the volume grows
 faster than the surface area
 Small organisms rely on diffusion
 Large organisms respiratory and excretory
 systems
4.4 Perpetuating Life

A species must reproduce or vanish from the
 planet
 Produce a new offspring
 Pass on the genetic information
A. Modes of Reproduction
Cells reproduce through cell division
 Cell fission in prokaryotes; mitosis in
 eukaryotes
 Results in identical daughter cells
1. Asexual Reproduction
No partner
 Offspring are genetically identical – clones
 Most single-celled organisms reproduce this way
 Some multicellular organisms do:
 Some sea anemones will split in half, making two
 smaller ones
 –fission
 Some sponges develop growths that break off to
 become separate individuals – budding or
 vegetative reproduction
2. Sexual Reproduction
 Union of two separate gametes from two parents
 Ovaries – female gonads that produce eggs
 Testes – male gonads that produce sperm
 Meiosis divides the chromosomes in half; Fertilization
  combines them to form a full
 set again
 A fertilized egg is called a zygote. It has DNA from both
  parents
 This genetic recombination causes variation in the
  offspring
 Greatest advantage of sexual reproduction
 The zygote divides by mitosis and eventually forms an
  embryo
 May pass through a larval stage on the way to adulthood
B. Reproductive Strategies
The goal of reproduction is to pass on the genes
 Varying reproductive strategies to get the same
 result
 Broadcast spawning – release millions of eggs
 and sperm into the water
 No parental care, most die
 Have few offspring and invest more time and
 energy into their survival
 Some use sexual and asexual reproduction
 Some species are hermaphroditic, both sexual
 organs
4.5 The Diversity of Life in the Sea
• The vast diversity of organisms in the ocean
  came through millions of years of evolution
• The gradual alteration of a species’ genetic
  makeup
A. Natural Selection and Adaptation
Individual organisms show variation in how they:
 Find food, avoid being eaten, reproduce, find
 mates, metabolize, etc.
 The best-adapted produce more offspring than
 the others
 This process is called natural selection
 As their genes get passed on the favorable traits
 become more common
 The population’s genetic makeup changes over
 time as it adapts to its environment
 Populations either adapt to the changes in the
 environment or become extinct
B. Classifying Living Things

To discuss the huge variety of life forms we
  must first classify them
1. The Biological Species Concept
 What is a species? A type of organism?
 A population with common characteristics
  that can successfully breed with each other
  (fertile offspring)
 If two populations cannot interbreed they are
  reproductively isolated
2. Biological Nomenclature
• Organisms are identified with a two-word
  name - Genus and species
• Blue whale – Balaenoptera musculus
• Fin whale – Balaenoptera physalus
• Minke whale – Balaenoptera acutorostrata
• Latin or Greek is used for naming
• Common names are confusing, scientific
  names are used worldwide to precisely
  identify a species
Levels of Classification
   Domain
   Kingdom
   Phylum
   Class
   Order
   Family
   Genus
   Species
3. Phylogenetics: Reconstructing Evolution
 Organisms are grouped according to their
  relatedness
  Related organisms share an evolutionary
  history, or phylogeny
  They share a common ancestor
  Look at fossil record, anatomy, reproduction,
 embryological development, DNA, behavior,
  etc.
4. The Tree of Life

• Classifications have changed over time
• Started with two kingdoms – Animalia and
  Plantae
• Then five kingdoms – added Fungi, Monera, &
  Protista
• Then three domain system
Fundamentals of biology

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Fundamentals of biology

  • 2. 4.1 The Ingredients of Life • A. The Building Blocks • 1 Organic compounds – molecules containing C, H, & O • Make life possible • High-energy molecules  Energy used to synthesize  Energy released in breakdown • Four types of organic compounds
  • 3. 2 Carbohydrates – sugars • Glucose – metabolized for energy • Starches – long chains of simple sugars used for energy storage • Chitin – skeletal material • Cellulose – cell structure
  • 4. 3 Proteins • Chains of amino acids • Muscles are mostly made up of proteins • Enzymes – catalyze reactions • Structural proteins – skin, hair, skeleton • Hormones
  • 5. 4 Lipids • Fats, oils, & waxes • Energy storage – more than twice as much as sugar • Water repellant • Buoyancy • Insulation • Hormones
  • 6. 5 Nucleic Acids • Store and transmit the genetic information of all living things • Long chains of subunits called nucleotides • DNA – instructions for the construction and maintenance of an organism; the complete set is called the genome • The nitrogen bases are sequenced into genes that code for a specific protein • RNA – helps DNA
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  • 11. B. The Fuel of Life • ATP – the molecule used to store energy; like a rechargeable battery • You use ~ 125 lbs./day • Organisms need to capture, store and use energy • Most organisms use only two sets of reactions
  • 12. 1 Photosynthesis: Making the Fuel • Algae, plants, and some microorganisms • Capture the sun’s energy and use it to make glucose  The pigment chlorophyll captures the solar energy  CO2 + H2O → C6H12O6 (glucose) + O2 • We rely on photosynthesis for food and oxygen • Organisms that photosynthesize are called autotrophs  Plants on land; bacteria and algae in the ocean
  • 13. 2 Respiration: Burning the Fuel • Both autotrophs and heterotrophs do it • Releases the energy from org. compounds • Reverse of photosynthesis  Organic matter (glucose) + O2→ H2O + CO2 • Similar to burning wood or oil • Chemical energy captured in ATP • Aerobic – uses oxygen, more efficient • Anaerobic – does not use oxygen, less efficient
  • 14. 3 Primary Production • Most of the glucose is used for fuel or converted into other types of org. compounds • The organic matter autotrophs make is called primary production  Used by the organism for growth and reproduction • Autotrophs are also called producers
  • 15. 4 The Importance of Nutrients • Vitamins, minerals and other substances are needed to convert glucose into other org. compounds • Nitrogen for proteins & nucleic acids • Phosphorus for nucleic acids • Silica (SiO2) to make shells • Iron – necessary, but a limited resource in the ocean
  • 16. 4.2 Living Machinery • Organic compounds are organized into functional units that are alive
  • 17. A. Cells and Organelles Cell – basic unit of life  All organisms are made of cells  Wrapped in a cell membrane • Cell is filled with jelly-like cytoplasm • Organelles have specific jobs in the cell
  • 18. 1. Structurally Simple Cells: Prokaryotes Prokaryotes are primitive cells  Ancient, simple, small  No membrane-bound organelles  Bacteria Prokaryotes have few structures:  Cell wall – support  Ribosomes – assemble proteins  DNA – loose in the cytoplasm  Flagella – locomotion
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  • 20. 2. Structurally Complex Cells: Eukaryotes Eukaryotic cells are organized and complex  Larger than prokaryotes Have specialized organelles:  Nucleus – contains chromosomes (DNA)  Endoplasmic reticulum – make proteins and other org. molecules for the cell  Golgi apparatus – package and transport molecules  Mitochondria – respiration center to provide energy  Flagella and cilia – for movement Only in plant & algal cells  Chloroplasts – photosynthesis center  Cell wall - support
  • 21. B. Levels of Organization 1. A cell is self-contained and can carry out all the functions necessary for life • Unicellular – all prokaryotes and some eukaryotes • Multicellular – most eukaryotes  Human body has 100,000,000,000,000 cells • In multicellular organisms cells specialize to perform different tasks for the organism • Cells that act together for a specific job are called tissues  Muscle, nervous, bone, blood, epithelial • Tissues are organized into organs to carry out specific functions  Liver, kidney, heart, skin, brain • Organs act together in an organ system  Skeletal, muscular, excretory, endocrine, digestive
  • 22. 2. Organization exists outside the individual organism  Species – one type of organism  Blue mussel  Population – a group of one species  A bunch of blue mussels  Community – several different populations that live and interact in an area  Blue mussels, crabs, barnacles, & chitons living on a rock  Ecosystem – the communities living together with the physical environment  Living on a rocky shore with seawater, air, temperature, sunlight, etc.
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  • 24. 4.3 Challenges of Life in the Sea • Marine organisms must cope with different problems than on land • They have evolved ways to adapt to their marine habitat • Most important is maintaining homeostasis Keeping their internal condition normal regardless of the external condition
  • 25. A. Salinity • Marine organisms are immersed in a medium – sea water – that can greatly affect their cell function • Enzymes and organic molecules are sensitive to ion concentration (salinity)
  • 26. 1. Diffusion and Osmosis • Dissolved ions move around in water • Random movement spreads them out in an even distribution • Results in diffusion – movement from high low concentration • When concentrations are different inside and outside a cell, substances will move in/out by diffusion  Salt from seawater will diffuse into the cell  Nutrients will diffuse out of the cell • The cell membrane blocks block diffusion  It’s selectively permeable – it allows only some substances to go in/out
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  • 28. • Water is a small molecule and can fit through the cell membrane  It also diffuses from high → low concentration  If a cell has more solutes inside than outside, water will stream in and swell the cell  If the seawater has more salt, water will leave and the cell will shrivel  This diffusion of water is called osmosis • Cells may need to move materials against diffusion (low high)  e.g. expelling extra salt or taking in more sugar  Active transport – proteins in the cell membrane pump materials using ATP 1/3 of the cell’s energy is spent on this
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  • 30. 2. Regulation of Salt and Water Balance • Marine organisms have adapted ways to balance water and salt • Osmoconformers –their internal concentrations change with the salinity of the seawater  Live in a narrow range of salinity • Osmoregulators – control internal concentrations to avoid osmotic problems  Can tolerate changes in salinity better  Can change their internal concentrations to match the seawater  Salt water fishes lose water by osmosis  Drink water or reduce urine amount to replace lost water  Excrete excess salts in the urine or through the gills
  • 31.  Freshwater fishes gain water by osmosis Don’t drink water or produce lots of urine Salt absorbed by gills  Some marine birds and reptiles have special glands to get rid of excess salt  Most algae have rigid cell walls that resist the swelling caused by osmotic water gain
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  • 33. B. Temperature  Metabolic reactions speed up/slow down when temperature goes up/down  Metabolic rate doubles every 10oC  At extreme temps most enzymes cease to function  Marine organisms are adapted to live in a temp range  Thus determining what regions of the oceans they live
  • 34.  Ectotherms – “cold blooded” lose their heat to the seawater  Endotherms – “warm blooded” retain heat and keep their body temp higher than the water  Mammals, birds, and some large fishes  Poikilotherms – body temp changes with the temp of the seawater  Incl. all ectotherms & endothermic fishes  Homeotherms – keep internal temp the same, regardless of outside temp  Produce more heat as need to keep their metabolic activity high  Mammals & birds  They need to eat more food  Insulate their bodies with feathers, hair, and blubber
  • 35. C. Surface-to-Volume Ratio  Heat and materials exchange across the surface of an organism  The surface-to-volume ratio (S/V ratio) determines how rapidly this happens  As organisms get larger the volume grows faster than the surface area  Small organisms rely on diffusion  Large organisms respiratory and excretory systems
  • 36. 4.4 Perpetuating Life A species must reproduce or vanish from the planet  Produce a new offspring  Pass on the genetic information
  • 37. A. Modes of Reproduction Cells reproduce through cell division  Cell fission in prokaryotes; mitosis in eukaryotes  Results in identical daughter cells
  • 38. 1. Asexual Reproduction No partner  Offspring are genetically identical – clones  Most single-celled organisms reproduce this way  Some multicellular organisms do:  Some sea anemones will split in half, making two smaller ones –fission  Some sponges develop growths that break off to become separate individuals – budding or vegetative reproduction
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  • 40. 2. Sexual Reproduction  Union of two separate gametes from two parents  Ovaries – female gonads that produce eggs  Testes – male gonads that produce sperm  Meiosis divides the chromosomes in half; Fertilization combines them to form a full  set again  A fertilized egg is called a zygote. It has DNA from both parents  This genetic recombination causes variation in the offspring  Greatest advantage of sexual reproduction  The zygote divides by mitosis and eventually forms an embryo  May pass through a larval stage on the way to adulthood
  • 41. B. Reproductive Strategies The goal of reproduction is to pass on the genes  Varying reproductive strategies to get the same result  Broadcast spawning – release millions of eggs and sperm into the water  No parental care, most die  Have few offspring and invest more time and energy into their survival  Some use sexual and asexual reproduction  Some species are hermaphroditic, both sexual organs
  • 42. 4.5 The Diversity of Life in the Sea • The vast diversity of organisms in the ocean came through millions of years of evolution • The gradual alteration of a species’ genetic makeup
  • 43. A. Natural Selection and Adaptation Individual organisms show variation in how they:  Find food, avoid being eaten, reproduce, find mates, metabolize, etc.  The best-adapted produce more offspring than the others  This process is called natural selection  As their genes get passed on the favorable traits become more common  The population’s genetic makeup changes over time as it adapts to its environment  Populations either adapt to the changes in the environment or become extinct
  • 44. B. Classifying Living Things To discuss the huge variety of life forms we must first classify them 1. The Biological Species Concept  What is a species? A type of organism?  A population with common characteristics that can successfully breed with each other (fertile offspring)  If two populations cannot interbreed they are reproductively isolated
  • 45. 2. Biological Nomenclature • Organisms are identified with a two-word name - Genus and species • Blue whale – Balaenoptera musculus • Fin whale – Balaenoptera physalus • Minke whale – Balaenoptera acutorostrata • Latin or Greek is used for naming • Common names are confusing, scientific names are used worldwide to precisely identify a species
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  • 47. Levels of Classification  Domain  Kingdom  Phylum  Class  Order  Family  Genus  Species
  • 48. 3. Phylogenetics: Reconstructing Evolution Organisms are grouped according to their relatedness  Related organisms share an evolutionary history, or phylogeny  They share a common ancestor  Look at fossil record, anatomy, reproduction, embryological development, DNA, behavior, etc.
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  • 50. 4. The Tree of Life • Classifications have changed over time • Started with two kingdoms – Animalia and Plantae • Then five kingdoms – added Fungi, Monera, & Protista • Then three domain system