Οι επιπτώσεις της ρύπανσης του Ασωπού ποταμού σε τρόφιμα - βολβούς. Έρευνα του Πανεπιστημίου Αθηνών που διενεργήθηκε το 2010 Tracing the Link Between Pollution and Food Production in the Asopos Area, GreeceChrysostomos G. Kirkillis [a], Ioannis N. Pasias [b], Sofia Miniadis-Meimaroglou [a], Nikolaos S. Thomaidis [b] and Ioannis Zabetakis [a]*  a University of Athens, Department of Chemistry, Food Chemistry Laboratory, Greece. b University of Athens, Department of Chemistry, Laboratory of Analytical Chemistry, Greece.

1. Tracing the Link Between Pollution and Food Production in the Asopos Area, Greece Chrysostomos G. Kirkillis [a], Ioannis N. Pasias [b], Sofia Miniadis-Meimaroglou [a], Nikolaos S. Thomaidis [b] and Ioannis Zabetakis [a]* a University of Athens, Department of Chemistry, Food Chemistry Laboratory, Greece. b University of Athens, Department of Chemistry, Laboratory of Analytical Chemistry, Greece. izabet@chem.uoa.gr

2. Miltos Kountouras, from Skopelos, Gera, Lesvos In front of “his school” the female Teachers’ Academy in Thessaloniki [1927]

3. “ Close the schools” Miltos Kountouras [1923]. Miltos using the nickname “ Evergetoulas” published his assay in the local newspaper “ Cabana” [the bell] edited by Stratis Mirivilis

5. Asopos – Oinofita (40km north of Athens…)

6. “ Background noise” Welding/metal – chemical – cosmetics – warehouses - food - farms

7. “ materials” and methods

8. Waste 2 the water-bed directly…

9. Materials and “Methods”

15. Table 1. Levels of trace element in carrots grown in the industrial area of Thiva, Central Greece (n=13x3=39) and from other areas, of low industrial activities (n=2x3=6). Detection limits ( μ g/kg wet wt): Cr 10, Pb 12, As 22 (524-882) (1.9-6.0) (29-40) (14-26) (80-106) Control (Range) d 703±253 4.0±2.8 35±8 20±8 93±18 Control (Mean, n=2) 524±51 6.0±0.6 29±2 26±4 80±2 Control 2 882±32 1.9±0.4 40±2 14±2 106±13 Control 1 (137-616) (3.0-25) (<12-43) (<10-77) (274-764) S(Range) d 410±183 11±6 21±14 43±22 474±148 S(Mean, n=13) 526±40 6.2±0.4 22±2 76±3 435±46 S13 350±31 8.0±1.0 14±1 68±6 550 ± 37 S12 576±32 15±1 13±1 25±4 764 ± 50 S11 616±85 10±1 22±1 44±1 53 2± 39 S10 562±83 10±1 43±7 41±3 4 20± 8 S9 553±32 8.2±1.0 43±6 36±1 436 ± 37 S8 395±13 13±1 37±2 28±1 689±249 S7 217±43 3.0±1.0 <12 <10 307 ± 27 S6 137±80 4.0±0.0 <12 47±14 328 ± 29 S5 167±37 25±1 <12 22±18 27 4± 16 S4 - 21±2 <12 33±3 45 9± 86 S3 - 11±3 33±1 61±6 36 8± 13 S2 <22 - 7.5±0.1 17±4 77±15 595 ± 71 S1 As c Cu Cd Pb b Cr a Ni Concentrations of metals (Mean±SD, μ g/kg wet wt) Carrots Samples (524-882) (1.9-6.0) (29-40) (14-26) (80-106) Control (Range) d 703±253 4.0±2.8 35±8 20±8 93±18 Control (Mean, n=2) (137-616) (3.0-25) (<12-43) (<10-77) (274-764) S(Range) d 410±183 11±6 21±14 43±22 474±148 S(Mean, n=13) As c Cu Cd Pb b Cr a Ni Concentrations of metals (Mean±SD, μ g/kg wet wt) Carrots Samples

16. Table 2. Levels of trace element in onions grown in the industrial area of Thiva, Central Greece (n=14x3=42) and from other areas, of low industrial activities (n=2x3=6). Detection limits ( μ g/kg wet wt): Cr 10, Pb 12, As 22 (605-621) (3.0-9.0) (22-27) (<10) (<45-82) Control (Range) e 613±11 6.0±4.9 24±3 <10 57±35 Control (Mean, n=2) 621±10 9.0±0.0 22±7 <10 82±0 Control 2 605±28 3.0±0.0 27±4 <10 <45 Control 1 (144-452) (1.0-11) (<12-72) (<10-26) (110-688) S(Range) e 317±94 5.3±3.2 35±18 <10 422±147 S(Mean, n=14) 389±38 4.0±0.0 52±2 <10 264±44 S14 252±30 7.7±0.1 24±5 <10 480±42 S13 261±32 2.7±0.6 43±6 <10 315±25 S12 361±44 2.0±0.0 35±3 <10 110±42 S11 452±46 8.0±1.0 28±2 <10 327±6 S10 205±14 4.0±1.0 35±7 <10 505±37 S9 421±29 3.0±0.0 26±11 <10 353±16 S8 144±15 4.0±0.0 45±11 <10 481±53 S7 292±11 5.4±0.3 62±8 <10 326±38 S6 315±32 11±2 13±1 <10 506±1 S5 297±20 11±1 26±3 16±4 539±66 S4 418±46 1.0±0.0 72±1 <10 469±27 S3 - 7.0±0.0 <12 <10 688±62 S2 <22 - 3.5±0.8 24±1 26±1 5 50±4 1 S1 As d Cu Cd Pb c Cr b Ni a Concentrations of metals (Mean±SD, μ g/kg wet wt) Onions Samples (605-621) (3.0-9.0) (22-27) (<10) (<45-82) Control (Range) e 613±11 6.0±4.9 24±3 <10 57±35 Control (Mean, n=2) (144-452) (1.0-11) (<12-72) (<10-26) (110-688) S(Range) e 317±94 5.3±3.2 35±18 <10 422±147 S(Mean, n=14) As d Cu Cd Pb c Cr b Ni a Concentrations of metals (Mean±SD, μ g/kg wet wt) Onions Samples

17. Table 3 . Levels of trace element in potatoes grown in the industrial area of Thiva, Central Greece (n=3x3=9) and from other areas, of low industrial activities (n=2x3=6). Detection limits ( μ g/kg wet wt): Cr 10, Pb 12, As 22 (925-1280) (5.1-34) (<12-30) (<10-14) (68-88) Control (Range) d 1102±251 20±21 18±17 <10 78±14 Control (Mean, n=2) 1280±52 5.1±0.5 <12 14±1 68±4 Control 2 925±48 34±4.1 30±5 <10 88±7 Control 1 (241-1459) (5.8-10) (<12-36) (<10-170) (90-1894) S(Range) d 881±611 7.3±2.2 16±17 63±93 800±961 S (Mean, n=3) 1459±65 10±1 <12 <10 416±12 S3 943±4 6.0±0.2 36±7 11±1 90±8 S2 <22 241±33 5.8±0.7 <12 170±9 1894±45 S1 As c Cu Cd Pb b Cr a Ni Concentrations of metals (Mean± SD, μ g/kg wet wt) Potatoes Samples (925-1280) (5.1-34) (<12-30) (<10-14) (68-88) Control (Range) d 1102±251 20±21 18±17 <10 78±14 Control (Mean, n=2) (241-1459) (5.8-10) (<12-36) (<10-170) (90-1894) S(Range) d 881±611 7.3±2.2 16±17 63±93 800±961 S (Mean, n=3) As c Cu Cd Pb b Cr a Ni Concentrations of metals (Mean± SD, μ g/kg wet wt) Potatoes Samples

19. Table 4. Trace element concentrations in vegetables from the industrial area of Asopos and Thiva, Central Greece, compared with previously results from other studies.

20. Table 5. Estimation of heavy metal daily intake through consumption of root vegetables in Thiva. a From Data Food Network (DAFNE, 2004). b Calculated by multiplying the mean levels of an element (left column for each metal) by the mean daily consumption of root vegetables (second column of the table).

22. 7 th AACD, 30.9.2010. Professor Aras on dietary intakes

24. A glimpse of the future…

26. Athanasios Panteloglou Chemist / Environmental Criminologist The man who discovered the “Tragedy of Asopos” …in 1999

27. Priest Ioannis Oikonomides [papaYannis] @ EU parliament, April 2009