Praktikum 25 - 26 Maret 2015 Infeksi Saluran Napas, Streptococcus, corynebacterium

1. INFEKSI SALURAN NAPAS
DICKY BAGUS WIDHYATMOKO
25 – 26 MARET 2015
DEPARTEMEN MIKROBIOLOGI KLINIK, FAKULTAS KEDOKTERAN
UNIVERSITAS AIRLANGGA

2. POKOK BAHASAN
 Streptococcus spp
 Corynebacterium diphteriae
 Mycobacterium tuberculosis

3. DISEDIAKAN
 Streptococcus :
 S. viridans
 S. pneumoniae
 S. pyogenes
 Corynebacterium spp
 M. tuberculosis
 Gram Stain
 Blood Agar
 Chocolate Agar
 Optochin Test
 Bacitracin test
 PAI medium
 Neisser Stain (AB, C)
 Ziehl-Neelsen Stain
 prepared slide

4. Identifikasi
Streptococcus spp.
 Blood Agar
 Chocolate Agar
 Optochin Test
 Bacitracin test
 Zona hemolisis
 Antibiotic Sensitivity
Test for Identification

5. First step
 Gram Stain
 always do gram stain before continuing to others
test
 Catalase Test
 please learn from previous laboratory practice

6. Next Step : Hemolysis
  hemolysis
  hemolysis
  hemolysis

7.  hemolysis
 partial / incomplete hemolysis
 reduction red blood cell hemoglobin 
methemoglobin in the medium surrounding
the colony.
 The greenish color is caused by the presence
of biliverdin, which is a by-product of the
breakdown of hemoglobin
 e.g. S. pneumoniae, S. viridans groups

8.  hemolysis
Streptococcus viridans Streptococcus pneumoniae

9.  hemolysis
 complete or true lysis of red blood cells
 clear zone, approaching the color and
transparency of the base medium, surrounds
the colony
 e.g. S. pyogenes

10.  hemolysis
 Streptococcus pyogenes
 clear zone surround
colony

11.  hemolysis
 lack of hemolysis / non-hemolysis
 e.g. Enterococcus faecalis

12.  hemolysis
non hemolysis, Enterococcus faecalis

13. Hemolysis Resume
taken from : http://iws2.collin.edu/dcain/

14.  hemolysis
 how to differentiate between S. pneumoniae
with S. viridans
 Using
 Optochin test
 Inulin Test
 Bile Solubility Test

15. Optochin Test
 Antibiotics Susceptibility Test
 Optochin also known as ethyl-hydro-cupreine
 To Differentiate between S. pneumoniae with
S. viridans
 S > 14 mm : S. pneumoniae
 No zone inhibition : S. viridans

16. Optochin Test
taken from http://arabslab.com
S. pneumoniae 
Sensitive
S. viridans groups  no
zone inhibition

17. Inulin Test
 To differentiate between Streptococcus
pneumoniae with all others alpha-hemolytics
streptococci
 pneumoniae hydrolize inulin

18. Bile Solubility Test
 Bile (sodium deoxycholate)
 Distinguishes S. pneumoniae from all others
alpha-hemolytics streptococci
 Sodium deoxycholate (2% in water) will lyse
the pneumococcal cell wall

19. Bile Solubility Test

20.  for feedback mailto : me@dickybagus.my.id