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R.E.D. Laboratories N.V.
                   TEST REQUEST FORM                                                                                                               Z.1 Researchpark 100 - 1731 Zellik
                                                                                                                                                                            Belgium

                                                                                                                                                      BE 462 648 824 RPR BRUSSEL


Family name:                                                                   First name:
Address:

Zip Code:                                                                      City:                                     Country:

Phone:

Date of birth:                                                                 Sex:    Male / Female

Date and hour blood drawn:

Name of requesting physician:                                                                                            Your patient ref.:

Shipping charges We may take care of the shipment of your samples from the point of collection to our laboratory. There are no shipping charges for
samples collected in Belgium. For samples collected in certain countries we ask for a contribution to the shipping costs. Contact us for more information.


                                                                                       Immune Dysfunction
                 RNase L cleavage                                                               3 x 8 ml Na-heparin tubes shipped within 8 hours at room temperature, or 3 x 8 ml CPT-
□    RNAE        37/80 RNase L fragments ratio
                                                                                        179 €   heparin tubes, centrifuged, inverted 5-10 times, shipped within 24 hrs at room temperature

                 Proinflammatory cytokines
□    CYTS        IL-1, IL-6, IL-8, IL-10, IL-12p70, TNF,                               179 €   2 ml serum, centrifuged within 2 hours. Aliquot and store at –80°C
                 TGF-1, MIP-1 MCP1

                 Th1/Th2 cytokines
□    CYTH        IL-2, IL-4, IFN mRNA levels
                                                                                        130 €

                 Cytokine RNA, inflammatory
□    CYTI        IL-6, IL-8, IL-10, TNF, TGF-1, MIP-1                                 179 €
                                                                                                One Paxgene™ tube. Stable for 72 hours at ambient temperature.
                 mRNA levels
                                                                                                For longer periods freeze and ship at –20°C
                 Perforin expression
□    PERF        NK cell activity
                                                                                        115 €

                 Elastase expression
□    ELAS        Neutrophil activation
                                                                                        115 €

                 CD57 absolute count                                                            2 ml whole blood in EDTA tube. Keep at ambient temperature or 4°C.
□    CD57        Absolute number of CD57+/CD3– cells
                                                                                        68 €    Stable for 48 hours. Do not freeze

                 Immunophenotyping                                                              5 ml whole blood in EDTA tube. Keep at ambient temperature or 4°C.
□    IMPH        CD4, CD8 and CD56 determination
                                                                                        68 €    Stable for 48 hours. Do not freeze

□    SCD14       Soluble CD14                                                           72 €
                                                                                                2 ml serum. Stable for 24 hours. For longer periods store at –20° / –80°C
□    C4AS        C4A serum level                                                        85 €


                                                          Environmental Exposure, Oxidative Stress
                                                                                                2 x 8 ml whole blood in Na-heparin tube, shipped within 24 hours (keep at ambient
□    PrPc        PrPc functional testing                                                90 €    temperature)

                 MELISA® Nickel & Mercury                                                       20 ml whole blood in Na-Citrate tube, shipped within 48 hours (keep at ambient
□    MEL1        ATEGIS TEST                                                            160 €   temperature). Send tubes Monday-Tuesday-Wednesday only
                 Hg-MeHg-PhHg-Ni

                 28-element urine profile
□    METU        Al, Sb, As, Ba, Be, Bi, Pb, Ce, Ga, Cd, Ni, Pd, Pt, Hg, Pr,
                 Re, Rh, Ru, Ag, Te, Tl, Sn, W, U, Zr, Cu, Se, Zn
                                                                                        85 €    10-15 mL urine in urine container, after chelation therapy, room temperature


□    ANOX        Total antioxidant capacity                                             85 €

□    IMOX        IgA/IgM oxidative products                                             68 €    2 ml serum. Stable for 24 hours. For longer periods store at –20° / –80°C

                 MDA-TBARS
□    OXFA        Fatty acids oxidation
                                                                                        30 €


                                                                                 Genotyping VDR, MTHFR
                 VDR polymorphisms
□    GVDR        Fok1, Bsm1
                                                                                        68 €
                                                                                                2 ml whole blood in EDTA tube. Stable for 48 hours.
                                                                                                For longer periods freeze at –20°C / –80°C
                 Methylation cycle polymorphisms
□    GFOL        MTHFR 677C/T and 1298A/C
                                                                                        68 €


Phone: + 32 (0)2 481 53 10 (from 9 a.m to 4 p.m, Monday to Friday)                                                                                          Email: info@redlabs.com
Fax: +32 (0)2 481 53 11                                                                                                                                              www.redlabs.com
Viral infections
                                                                                                                Cost of PCR test:
             Human Herpesvirus 6
□   HHV6     Includes detection of chromosomal integration   □   HHV7     Human Herpesvirus 7                   60 € for sample processing + 48 € per PCR

                                                                                                                Sample required:

□   EPBV     Epstein-Barr virus                              □   CYMV     Cytomegalovirus                       Blood: 2 ml whole blood in EDTA tube. Stable for 24 hours.
                                                                                                                For longer periods freeze at –20°C or –80°C

                                                                          Enterovirus                      Biopsy in a sterile, dry tube (no buffer or fixative of any kind).
□   PARV     Parvovirus B19                                  □   ENTV     ! RNA: special collection tube ! Freeze at –20°C or –80°C within 6 hours

                                                                                                           PCR tests marked as «RNA» (WENV, COXV, ENTV)
             West Nile virus                                              Coxsackie virus
□   WENV     ! RNA: special collection tube !                □   COXV                                      require special collection tubes.
                                                                          ! RNA: special collection tube ! Please contact us for more information



                                                                                   Molds
□   CALB     Candida albicans IgG                            □   CLAH    Cladosporium herbarum IgG

                                                                                                                  Cost each determination: 30 €
□   ASPN     Aspergillus niger IgG                           □   ALTA    Alternaria alternata IgG                 Cost panel: 140 €

                                                                                                                  Sample required: 2 ml serum. Stable for 24 hours at ambient
□   PENC     Penicillium chrysogenum IgG                     □   ASPF    Aspergillus fumigatus IgG                temperature, for one week at 4°C. For longer periods store at
                                                                                                                  –20° / –80°C

□   MOLP     Molds IgG panel (CALB+ASPN+PENC+CLAH+ALTA+ASPF)


                                                             Zoonosis, Mycoplasma, Chlamydia
PCR TESTS

             Chlamydia pneumoniae                                        Chlamydia trachomatis
□   CHLP     Preferably sputum                               □   CHLT    Preferably swab

                                                                                                                  Cost of PCR test:
□   MYCP     Mycoplasma spp.                                 □   BRUC    Brucella spp.                            60 € for sample processing + 48 € per PCR

                                                                                                                  Sample required:
□   EHRL     Ehrlichia chaffeensis - canis                   □   BORB    Borrelia burgdorferi sl
                                                                                                                  Blood: 2 ml whole blood in EDTA tube. Stable for 24 hours.
                                                                                                                  For longer periods freeze at –20°C or –80°C
□   BART     Bartonella spp.                                 □   BABE    Babesia spp.
                                                                                                                  Sputum, swab: sample in a sterile, dry tube (no buffer or fixati-
                                                                                                                  ve of any kind). Stable for 24 hours. For longer periods freeze
□   ANAP     Anaplasma phagocytophilum                       □   RICK    Rickettsia spp.
                                                                                                                  at –20°C or –80°C

□   COXI     Coxiella spp.

SEROLOGY

             Zoonosis screening (serology)                               Cost 115 €
□   ZONP     Bartonella henselae, Borrelia burgdorferi,                  Sample required: 2 ml serum. Stable for 24 hours at ambient temperature, for one week at 4°C. For
             Coxiella burnetii, Rickettsia conorii                       longer periods store at –20° / –80°C

             BORCG
             Confirmation of Borrelia positivity (western blot). Only done if Borrelia EIA is positive. No additional sample required. Additional cost: 96 €


                                                                        Intestinal Function
              IgA/IgM intestinal bacteria (leaky gut)
              Detection of IgA/IgM directed against Klebsiella
□   IMBA      pneumoniae, Proteus mirabilis, Pseudomonas                  68 €
              aeruginosa, Citrobacter koseri, Pseudomonas                                  2 ml serum. Stable for 24 hours at ambient temperature, for one week at 4°C. For longer
              putida, Morganella morganii, Hafnia alvei                                    periods store at –20° / –80°C

              Tissue transglutaminase and Gliadin Ab
□   CELI      tTg IgG and IgA; GliadinDP IgG and IgA
                                                                          68 €

              Genetics of intestinal inflammation
□   IDGP      Polymorphisms of ATG16L1, IL-23R, IL-17F,                  115 €
                                                                                           2 ml whole blood in EDTA tube. Stable for 48 hours.
              TLR4, IL-6
                                                                                           For longer periods freeze at –20°C / –80°C
              Genetic lactase deficiency
□   LACT      C/T-13910 polymorphism of the lactase gene
                                                                          68 €




I am aware that the laboratory has no RIZIV number. These tests are «research only»; interpretation must be done by a health care professional. The cost
for the requested analyses will be invoiced directly to me by R.E.D Laboratories, at the above mentionned rate. I hereby authorise R.E.D Laboratories to
possibly use my blood sample for research purposes.
       Signature:                                                                  Date:

A signed copy of this form must be sent together with your samples.                                                                         We do accept :

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RED Laboratories - Request Form 08-10IMMUen

  • 1. R.E.D. Laboratories N.V. TEST REQUEST FORM Z.1 Researchpark 100 - 1731 Zellik Belgium BE 462 648 824 RPR BRUSSEL Family name: First name: Address: Zip Code: City: Country: Phone: Date of birth: Sex: Male / Female Date and hour blood drawn: Name of requesting physician: Your patient ref.: Shipping charges We may take care of the shipment of your samples from the point of collection to our laboratory. There are no shipping charges for samples collected in Belgium. For samples collected in certain countries we ask for a contribution to the shipping costs. Contact us for more information. Immune Dysfunction RNase L cleavage 3 x 8 ml Na-heparin tubes shipped within 8 hours at room temperature, or 3 x 8 ml CPT- □ RNAE 37/80 RNase L fragments ratio 179 € heparin tubes, centrifuged, inverted 5-10 times, shipped within 24 hrs at room temperature Proinflammatory cytokines □ CYTS IL-1, IL-6, IL-8, IL-10, IL-12p70, TNF, 179 € 2 ml serum, centrifuged within 2 hours. Aliquot and store at –80°C TGF-1, MIP-1 MCP1 Th1/Th2 cytokines □ CYTH IL-2, IL-4, IFN mRNA levels 130 € Cytokine RNA, inflammatory □ CYTI IL-6, IL-8, IL-10, TNF, TGF-1, MIP-1 179 € One Paxgene™ tube. Stable for 72 hours at ambient temperature. mRNA levels For longer periods freeze and ship at –20°C Perforin expression □ PERF NK cell activity 115 € Elastase expression □ ELAS Neutrophil activation 115 € CD57 absolute count 2 ml whole blood in EDTA tube. Keep at ambient temperature or 4°C. □ CD57 Absolute number of CD57+/CD3– cells 68 € Stable for 48 hours. Do not freeze Immunophenotyping 5 ml whole blood in EDTA tube. Keep at ambient temperature or 4°C. □ IMPH CD4, CD8 and CD56 determination 68 € Stable for 48 hours. Do not freeze □ SCD14 Soluble CD14 72 € 2 ml serum. Stable for 24 hours. For longer periods store at –20° / –80°C □ C4AS C4A serum level 85 € Environmental Exposure, Oxidative Stress 2 x 8 ml whole blood in Na-heparin tube, shipped within 24 hours (keep at ambient □ PrPc PrPc functional testing 90 € temperature) MELISA® Nickel & Mercury 20 ml whole blood in Na-Citrate tube, shipped within 48 hours (keep at ambient □ MEL1 ATEGIS TEST 160 € temperature). Send tubes Monday-Tuesday-Wednesday only Hg-MeHg-PhHg-Ni 28-element urine profile □ METU Al, Sb, As, Ba, Be, Bi, Pb, Ce, Ga, Cd, Ni, Pd, Pt, Hg, Pr, Re, Rh, Ru, Ag, Te, Tl, Sn, W, U, Zr, Cu, Se, Zn 85 € 10-15 mL urine in urine container, after chelation therapy, room temperature □ ANOX Total antioxidant capacity 85 € □ IMOX IgA/IgM oxidative products 68 € 2 ml serum. Stable for 24 hours. For longer periods store at –20° / –80°C MDA-TBARS □ OXFA Fatty acids oxidation 30 € Genotyping VDR, MTHFR VDR polymorphisms □ GVDR Fok1, Bsm1 68 € 2 ml whole blood in EDTA tube. Stable for 48 hours. For longer periods freeze at –20°C / –80°C Methylation cycle polymorphisms □ GFOL MTHFR 677C/T and 1298A/C 68 € Phone: + 32 (0)2 481 53 10 (from 9 a.m to 4 p.m, Monday to Friday) Email: info@redlabs.com Fax: +32 (0)2 481 53 11 www.redlabs.com
  • 2. Viral infections Cost of PCR test: Human Herpesvirus 6 □ HHV6 Includes detection of chromosomal integration □ HHV7 Human Herpesvirus 7 60 € for sample processing + 48 € per PCR Sample required: □ EPBV Epstein-Barr virus □ CYMV Cytomegalovirus Blood: 2 ml whole blood in EDTA tube. Stable for 24 hours. For longer periods freeze at –20°C or –80°C Enterovirus Biopsy in a sterile, dry tube (no buffer or fixative of any kind). □ PARV Parvovirus B19 □ ENTV ! RNA: special collection tube ! Freeze at –20°C or –80°C within 6 hours PCR tests marked as «RNA» (WENV, COXV, ENTV) West Nile virus Coxsackie virus □ WENV ! RNA: special collection tube ! □ COXV require special collection tubes. ! RNA: special collection tube ! Please contact us for more information Molds □ CALB Candida albicans IgG □ CLAH Cladosporium herbarum IgG Cost each determination: 30 € □ ASPN Aspergillus niger IgG □ ALTA Alternaria alternata IgG Cost panel: 140 € Sample required: 2 ml serum. Stable for 24 hours at ambient □ PENC Penicillium chrysogenum IgG □ ASPF Aspergillus fumigatus IgG temperature, for one week at 4°C. For longer periods store at –20° / –80°C □ MOLP Molds IgG panel (CALB+ASPN+PENC+CLAH+ALTA+ASPF) Zoonosis, Mycoplasma, Chlamydia PCR TESTS Chlamydia pneumoniae Chlamydia trachomatis □ CHLP Preferably sputum □ CHLT Preferably swab Cost of PCR test: □ MYCP Mycoplasma spp. □ BRUC Brucella spp. 60 € for sample processing + 48 € per PCR Sample required: □ EHRL Ehrlichia chaffeensis - canis □ BORB Borrelia burgdorferi sl Blood: 2 ml whole blood in EDTA tube. Stable for 24 hours. For longer periods freeze at –20°C or –80°C □ BART Bartonella spp. □ BABE Babesia spp. Sputum, swab: sample in a sterile, dry tube (no buffer or fixati- ve of any kind). Stable for 24 hours. For longer periods freeze □ ANAP Anaplasma phagocytophilum □ RICK Rickettsia spp. at –20°C or –80°C □ COXI Coxiella spp. SEROLOGY Zoonosis screening (serology) Cost 115 € □ ZONP Bartonella henselae, Borrelia burgdorferi, Sample required: 2 ml serum. Stable for 24 hours at ambient temperature, for one week at 4°C. For Coxiella burnetii, Rickettsia conorii longer periods store at –20° / –80°C BORCG Confirmation of Borrelia positivity (western blot). Only done if Borrelia EIA is positive. No additional sample required. Additional cost: 96 € Intestinal Function IgA/IgM intestinal bacteria (leaky gut) Detection of IgA/IgM directed against Klebsiella □ IMBA pneumoniae, Proteus mirabilis, Pseudomonas 68 € aeruginosa, Citrobacter koseri, Pseudomonas 2 ml serum. Stable for 24 hours at ambient temperature, for one week at 4°C. For longer putida, Morganella morganii, Hafnia alvei periods store at –20° / –80°C Tissue transglutaminase and Gliadin Ab □ CELI tTg IgG and IgA; GliadinDP IgG and IgA 68 € Genetics of intestinal inflammation □ IDGP Polymorphisms of ATG16L1, IL-23R, IL-17F, 115 € 2 ml whole blood in EDTA tube. Stable for 48 hours. TLR4, IL-6 For longer periods freeze at –20°C / –80°C Genetic lactase deficiency □ LACT C/T-13910 polymorphism of the lactase gene 68 € I am aware that the laboratory has no RIZIV number. These tests are «research only»; interpretation must be done by a health care professional. The cost for the requested analyses will be invoiced directly to me by R.E.D Laboratories, at the above mentionned rate. I hereby authorise R.E.D Laboratories to possibly use my blood sample for research purposes. Signature: Date: A signed copy of this form must be sent together with your samples. We do accept :