4.11.24 Mass Incarceration and the New Jim Crow.pptx
Metabolism protein and fat
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METABOLISM OF FOOD LOGO
Dr. Daxaben N. Mehta
Principal
Smt. S.C.U.Shah Home Science and
C.U.Shah Arts & Commerce Mahila College
Wadhwancity – Dist: Surendranagar
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AMINO ACID Broadcasting
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ENVIRONMENT Bio- ORGANISM
synthesis Protein
Ingested
protein
AMINO
ACIDS Purines
c c
Degradation Pyrimidines
(required) Carbon Porphyrins
Nitrogen skeletons
(ketogenic) (glucogenic)
Urea Used for
pyruvate
energy α-ketoglutarate
acetoacetate succinyl-CoA
acetyl CoA fumarate
oxaloacetate
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TRANSAMINATION LOGO
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UREA CYCLE LOGO
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FAT METABOLISM LOGO
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7. β - OXIDATION
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Breakdown of fats into
Acetyl coenzyme A --> Krebs Cycle
FADH2 --> Oxidative Phosphorylation
NADH--> Oxidative Phosphorylation
Breaks off two carbons at a time to acetyl
CoA
Remaining goes another round
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8. Prepares a Fatty Acid for transport and metabolism
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CH3CH2CH2CH2CH2COOH
ATP
PPi
[CH3CH2CH2CH2CH2CO-AMP] Fatty acyl
CoA Ligase
HS-CoA
AMP
CH3CH2CH2CH2CH2CO~SCoA
Fatty acyl CoA
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Beta-Oxidation of Fatty Acids
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In reaction 1, oxidation:
• Removes H atoms from the
and carbons.
• Forms a trans C=C bond.
• Reduces FAD to FADH2.
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Beta-Oxidation of Fatty Acids
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In reaction 2, hydration:
• Adds water across the
trans C=C bond.
• Forms a hydroxyl group
(—OH) on the
carbon.
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11. Beta ()-Oxidation of Fatty Acids
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LOGO
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In reaction 3, a second
oxidation:
• Oxidizes the hydroxyl
group.
• Forms a keto group
on the carbon.
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12. Beta ()-Oxidation of Fatty Acids
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LOGO
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In Reaction 4, acetyl
CoA is cleaved:
• By splitting the bond
between the and
carbons.
• To form a shortened
fatty acyl CoA that
repeats steps 1 - 4 of
-oxidation.
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MECHANISM LOGO
CH3CH2CH2CH2CH2CO~SCoA Fatty Acyl CoA
FAD
Fatty Acyl Dehydrogenase
FADH
CH3CH2CH=CHCH2CO~SCoA β Enoyl Co. A
H2O
Enoyl Co. A Hydratase
OH
CH3CH2CH2CHCH2CO~SCoA β Hydroxy Acyl Co. A
β Hydroxy acyl Co. A Dehydrogenase NAD+
O
NADH
CH3CH2CH2CCH2CO~SCoA β Keto Acyl Co. A
HSCo A
Thiolase
Fatty Acyl CoA CH3CH2CH2CO~SCoA CH2CO~SCoA Acetyl CoA
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6 carbon Fatty Acid Acyl-CoA
CH3CH2CH2CH2CH2CH2CH2C~SCoA
O
CH3C~SCoA
O
CH3C~SCoA
O CH3C~SCoA
O
3 two carbon Acetyl-CoAs
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ENERGY PRODUCTION LOGO
COMPARISON BETWEEN
Hexanoic Acid C6H12O2 Glucose C6H12O6
Hexanoic acid Glucose
Hexanoil Co. A - 1 ATP Pyruvate 2 ATP
6 NADH
Hexanoil Co. A Pyruvate
3 Acetyl-CoA 36 ATP 2 Acetyl-CoA 24 ATP
2 FADH2 4 ATP
2 NADH + H+ 6 ATP 2 NADH + H+ 6 ATP
45 ATP 38 ATP
MW 116 MW 180
ATP per Gram 0.32 ATP per Gram 0.17
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Oxidation of Unsaturated Fatty
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Acids.
• Oxidation of monounsaturated fatty acyl-CoA
requires additional reaction performed with the
help of the enzyme isomerase.
• Double bonds in the unsaturated fatty acids are in
the cis configuration and cannot be acted upon
by enoyl-CoA hydratase (the enzyme catalyzing
the addition of water to the trans double bond
generated during β-oxidation.
• Enoyl-CoA isomerase repositions the double
bond, converting the cis isomer to trans isomer, a
normal intermediate in β-oxidation.
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Oxidation of polyunsaturated
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fatty acids.
• Requires two additional reactions and a
second enzyme, reductase, in addition to
isomerase.
• NADPH-dependent 2,4-dienoyl-CoA
reductase converts trans-2, cis-4-dienoyl-
CoA intermediate into the trans-2-enoyl-
CoA substrate necessary for β-oxidation.
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fatty acids.
• Odd-carbon fatty acids are oxidized by the same pathway as
even-carbon acids until three-carbon propionyl-CoA is formed.
• After that, three additional reactions are required involving three
enzymes.
• Propionyl-CoA is carboxylated by propionyl-CoA carboxylase
(with the cofactor biotin) to form the D stereoisomer of
methylmalonyl-CoA (The formation of the carboxybiotin
intermediate requires energy from ATP).
• D-methylmalonyl-CoA is changed into L-methylmalonyl-CoA by
methylmalonyl-CoA epimerase.
• L-methylmalonyl-CoA undergoes an intramolecular
rearrangment to form succinyl-CoA, which enters the citric acid
cycle. This rearrangment is catalyzed by methylmalonyl-CoA
mutase, which requires coenzyme B12, derived from vitamin B12
(cobalamin).
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Ketone Bodies LOGO
A special source of fuel and energy for certain tissues
• Some of the acetyl-CoA produced by fatty acid
oxidation in liver mitochondria is converted to
acetone, acetoacetate and -hydroxybutyrate
• These are called "ketone bodies"
• Source of fuel for brain, heart and muscle
• Major energy source for brain during starvation
• Synthesis in Figure 24.28
• They are transportable forms of fatty acids!
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Ketone Bodies and Diabetes
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LOGO
"Starvation of cells in the midst of plenty"
• Glucose is abundant in blood, but uptake by cells in
muscle, liver, and adipose cells is low
• Cells, metabolically starved, turn to gluconeogenesis
and fat/protein catabolism
• In type I diabetics, OAA is low, due to excess
gluconeogenesis, so Ac-CoA from fat/protein
catabolism does not go to TCA, but rather to ketone
body production
• Acetone can be detected on breath of type I
diabetics
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