![What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components. Separate ,[object Object],[object Object],[object Object],[object Object],Components Mixture](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)
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Paper Chromatography
- 7. Illustration of Chromatography Mixture Components Separation Stationary Phase Mobile Phase Components Affinity to Stationary Phase Affinity to Mobile Phase Blue ---------------- Insoluble in Mobile Phase Black Red Yellow
- 10. (A) uses charge, (B) uses pores, and (C) uses covalent bonds to create the differential affinities among the mixture components for the stationary phase.
- 12. Paper Chromatography Experiment What Color is that Sharpie?
- 21. Observing the Chromatograms Concentration of Isopropanol 0% 20% 50% 70% 100%
- 23. Blue Dye Concentration of Isopropanol 0% 20% 50% 70% 100% 1. Dye separated – blue 2. Not very soluble in low concentrations of isopropanol 3. Completely soluble in high concentrations of isopropanol
- 24. Green Dye Concentration of Isopropanol 0% 20% 50% 70% 100% 1. Dye separated – blue and yellow 2. Blue – Soluble in concentrations of isopropanol >20% 3. Yellow – Soluble in concentrations of isopropanol >0%