1. Cameron Martins
Amazing
Review
Rated R
No child under 17 will be permitted
with out a adult

2. Lab one
• Special Media: The two other types of media you
will see in class today are enriched and
selective/differential. Enriched media is used to
culture fastidious organisms like Streptococcus
pyogenes. The example in the laboratory is blood
agar which contains 5% sheep blood.
• Selective media allows for some bacteria to grow
and inhibits others. Differential media allows
bacteria to grow based on some biochemical or
physical property that allows the bacteria to be
differentiated from each other.

5. Lab two
Spread plate technique

6. Lab two
• Spread plate technique
• Uses a series of dilutions to dilute the bacteria
• Usually uses broth of a saline solution.
• Uses alcohol and a hockey stick to spread.
• The equation used is

7. Lab two
Streak plate technique

8. Lab two
Streak plate technique
• This uses distance to dilute bacteria out
• Uses a inoculating loop to spread to dilute.

9. Lab 3
• Hanging Drop Slide
This slide is used to detect motion of the bacteria.
• Negative staining
Does not stain the bacteria it stains the
background. This stain is acidic.(ex nigrosin)
Simple Staining
Will stain the bacteria, this stain is basic. (ex. Crystal
violet)

10. Lab 3

11. Lab 3
Negative stain

12. Lab 3
simple stain

13. Lab 4
• Gram stain
Stains the cell wall of the organism this tells the type of cell
wall of the organism.Red cells gram – purple gram positive.
• Capsule stain
The maneval stain was used this told if the organism had a
capsule uses a negative stain to coat the outside of the cell.
A capsulated cell would have a clear space between the
two stains.
Endospore stain
This procedure you cooked in malachite green stain
For 5 min. then counter stain with safranin.
The red is vegetative cells and the green is endospores.

14. Gram stain

15. Capsule

16. Endospore stain

17. Gram negative Media
• EMB
• Macconkey agar
• Hektoen
• Urea

18. EMB
• A positive test means what ?
• growth of Gram - organisms. Additionally, the
medium contains lactose which can be utilized
by lactose fermenters as a carbon and energy
source.
• Grow means its what ?
• That it’s a gram -.

19. EMB

20. Macconkey’s
• What does growth mean ?
• That is a gram -
• What does positive test mean?
• Like EMB, this medium contains lactose and those
organisms that can utilize lactose produce acids. The acid
production results in a lower pH in the medium and a
change in the color of the medium to yellow. Neutral red is
the ph indicator
• This also has peptones in the media if it turns pink it means
that the organism is breaking down peptones.
• The selective molecule is bile salts

21. Macconkey agar

22. Hektoen enteric agar
• MacConkey’s agar. However, this differs from
MacConkey’s agar because it also has ammonium
sulfate and sodium thiosulfate in it.
• These chemicals are indicator molecules because they
react with H2S released from the breakdown of
peptones in the medium and turn the H2S producing
colonies black (indicating that H2S was present).
• The pH indicator in this medium is bromothymole blue.
• When bacteria use the lactose and sucrose in the
medium acid is produced and the medium’s color
changes from a blue/green
• It has bile salts as the selective indicator molecule

23. Hectoen enteric agar

24. Urea broth
• If its positive
• the organism has urease .
• If negative it doesn’t poses urease.
• Has phenol red as a ph indicator

25. Urea broth

26. Gram positive
• MSA
• PEA
• Mitis-salavarius

27. Mitis-salavarius
• Grows up mainly gram positive mainly
streptococcus.
• It incorporates the trypan blue from taking in
sucrose peptones and all the different
carbohydrates.
• This media incorporates crystal violet and
potassium tellurite which inhibits gram
negatives, and other non streptococcus
organisms.

28. Mitis- salavarius

29. PEA
• This media has a inhibitor molecule of phenol
ethyl alcohol.
• This molecule inhibits gram – organisms by
disrupting the LPS.

30. PEA

31. Mannitol salt agar
• IF an organims grows this means that the
organism is a halophile.
• .When the organism uses mannitol it changes
the PH of the media changing it yellow
• PH indicator phenol red
• It has NaCl as a inhibitory molecule.

32. Mannitol salt agar

33. Temperature
• Cardinal temperatures
These are the temperature which an organism
grows. This includes minimum maximum and
optimal temperatures.

34. temperature

35. Examples

36. Oxygen requirements

37. Example