Presented at Nordic Green II conference, April 2010

1. Industrial Biotechnology: on the
Road to Sustainable Biorefineries
for Biochemical and Biofuels
Production
Richard J. LaDuca
April 28, 2010
Nordic Green II
Conference
Palo Alto, CA

2. Genencor at a Glance….
We put Biotechnology to work… developing & Industrial
producing enzymes for industrial applications. Enzymes
Animal Feed Enzymes
Cellulosic Enzymes
History traced to 1982 Food Enzymes
Fabric & Household
May 2005: Division of Danisco Care
Grain Processing
Industrial Biotechnology Focus Textiles Enzymes
Over 250 commercial products
Biochemicals
Proven innovator: ~3,500+ Lysine
owned & licensed patents and Threonine
Tryptophan
patent applications 1,3 Propanediol
Indigo
H Biotin
> $800 MM USD annual turnover C C
CH3
Ascorbic Acid
1,3 Propanediol
~ 1,500 employees worldwide H2C CH2
BioIsoprene™
BioIsoprene™
2

3. Genencor® business timeline
A Leader in Industrial Biotechnology
Danisco acquires GCOR, 100%
Eastman Chemical 1993, Danisco
50% 1999, 50%
Dual Kodak-Cultor ownership that
forms:
Genencor International, Inc. GCOR buys Gist- Brocades Enzymes (Brugge, New Wuxi China plant and
BE) application laboratories
Genentech & Corning GCOR buys Solvay Enzyme (USA,
Argentina, Germany) Shanghai Research Center
form Genencor, Inc.
GCOR buys Wuxi Enzymes (China)
Cedar Rapids
GCOR buys Enzyme Bio-Systems, (USA) expansion and
establishment of
GCOR buys Rhodia Enzymes (UK) Grain CoE
1982 1990 1995 1996 1999 2002 2003 2005 2007 2008 2009
An early pioneer in modern industrial biotechnology
3

4. Genencor Biotechnology
Our Core Competency:
We design and operate Cell Factories
– We rapidly create engineered proteins that accomplish the previously
impossible and deliver significant value to our most important
customers.
– We engineer the metabolic pathways of micro-organisms to produce
enzymes, proteins, and other bio-molecules at industrial scale and
economics.
4

5. Industry Trends: Fuels / Chemicals
Petroleum-based Processes Biobased Processes
Existing, mature refining infrastructure; Building off of early successes using
starch-based feedstocks
high efficiencies, low cost
Enables a $5-10 billion dollar industry
Enables a $2+ trillion dollar chemical Driven by a renewable raw material
industry worldwide base
Volatility in oil price leads to volatility in Innovation and advances in enzyme
chemical / fuel prices processing efficiencies and costs
Feedstock source is non-renewable Trend towards: less GHG emissions;
less non-renewable energy usage; and
Innovation potential around existing reduced environmental impact
chemical processes has been played Move to waste biomass as a feed
out source
GHG / CO2 abatement concerns Technology innovation and systems
integration drive move to
BIOREFINERIES
5

6. Biorefinery:
“A biorefinery is a facility that converts waste
biomass into fuels, power, and chemicals.
The biorefinery concept is analogous to today's
petroleum refineries, which produce multiple fuels
and products from petroleum.”
- DOE 2008, Biomass Program
66

7. Industrial Biotechnology: delivering
technology enabling Biorefineries
Non-Renewable Renewable Renewable
Feedstocks Feedstocks Feedstocks
Crude Agricultural Biomass
Oil Products Wastes
Petro-Refining / Bio Bio
Cracking Refining Biorefineries Refining
using
E E
renewable raw
Fractionation Starch
Processing
Fractionation materials as
Cellulose
Processing
Fractionation
Enzymes
feedstocks are TODAY
Enzymes
Chem Building Starch C5/C6
Blocks
delivering products
C5/C6 Sugars,
Sugars Lignin
that compete with
Chem Syn. & Bio Bio
Conversion E Conversion
petroleum refining
E Conversion
Metabolic Metabolic
Pathway Pathway
Petro-based Engineering Biobased Biobased
Engineering
Products Products Products
Chemicals Fuels Biochemicals Biofuels Biochemicals Biofuels
Not Sustainable Sustainable Sustainable
Proven Method Proven Method In Development
Price Volatility Food Based Ag Wastes
Existing Infrastructure Existing Infrastructure No Existing Infrastructure
7

8. Genencor’s Road to Sustainable
BIOREFINERIES
Enzymes for starch processing
1st Generation Biochemicals & Bioethanol
2nd Generation Bioethanol
Renewable Biochemicals and Biofuels
98

9. Conventional 1st generation ethanol
production process
Thermo-stable E Alcohol
alpha amylase Glucoamylase Yeast recovery
Milo Liquefaction Saccharification Distillation &
Fermentation
Corn dehydration
Wheat Water
Rye JET COOKER
*
Barley >100° C
Tapioca 5–8 MIN
STORAGE
TANK
60° C
8–10 HRS
(optional)
GRINDING SLURRY
TANK
SECONDARY
LIQUEFACTION
95° C
*
~90 MIN
DDGS
pH adjustment steps are not shown
9

10. No-cook ethanol production process
Alcohol
recovery
Milo
Corn
Wheat Saccharification & Distillation &
Rye fermentation dehydration
Water
Barley
Tapioca
STORAGE
TANK
SLURRY
GRINDING TANK
*
STARGEN™
&
Yeast
DDGS
E
pH adjustment steps are not shown 10

11. Genencor & DuPont: 1st Generation
Biochemicals from Renewable Raw
Materials
E
11

12. 1st Generation Biochemicals
Genencor’s enzyme technology is at the heart of the
integrated bioprocess to produce 1,3 propanediol
12

13. Enzyme Activities Important
in Biomass Hydrolysis
Accellerase® DUET achieves 3- Many customers and pilot
fold dose reduction on multiple facilities are trialing Accellerase®
substrates
Genencor® is actively working
Accellerase® DUET enabling on future generations of
commercialization with $0.50 Accellerase® products.
enzyme cost / Gal EtOH
E
13

14. Total Sugar Conversion: Pretreated
Hardwood Pulp, 3 day Saccharification
E
0.17 mL Accellerase® DUET vs. 1.0 mL Accellerase® 1500 (5.9X)
14

15. 2nd Generation Advanced Biofuels
50/50 Joint Venture between Danisco and DuPont
Commercializing integrated technology to produce cellulosic ethanol
Over $140mm investment over 3 years
Biomass feedstock-flexible (utilizing cob and switchgrass in Eastern Tennessee)
pilot plant 2010
Commercial plant in 2012
E
15

16. Isoprene Production:
PETROLEUM CHEMICAL BIOBASED
TREES
CRACKING SYNTHESIS PROCESS
• Slow • Extractive • High cost • Renewable
distillation from feedstocks
• Not Ethylene & • Petroleum
commercially Propylene feedstocks • Biochemical
relevant production AND Biofuel
• Mfg. Products
• Price linked to infrastructure in
oil prices Russia & Japan
• Moving to
“light” streams 16

17. The Vision: Isoprene from Biomass
Biomass
Sugars
OH
Isoprene
O
HO
HO
Corn Switchgrass OH OH
D-Glucose
2-Methyl-1,3-butadiene
+
other C5 and C6
sugars
Sugar cane Woody
Molasses biomass
We aim to develop an efficient and sustainable fermentation
route to isoprene from carbohydrate feedstocks
17

18. Genencor and Goodyear leveraging
each others’ expertise …
H CH3
E E C C
H2C CH2
BioIsoprene™
Polymer-Grade
Renewable Microbial Strain Large Scale recovery &
BioIsoprene™
Feedstock(s) Development Fermentation purification
Product
knowhow
Integrated biobased process for production of BioIsoprene™
18

19. C5 Platform Progress – Stage I Biochemicals
BioIsoprene™ Technology
Conceptual View of Cell Factory
Glucose
C6 ►3C DXP Pathway
Sucrose / (C5)
Biomass Isoprene Isoprene
3C ►2C Cells Synthase Recovery
Glycerol
16C ►2C MVA Pathway
E
Plant Oils
Multiple feedstocks possible
Two feeder pathways to isoprene precursor
Isoprene synthase
Gas Phase recovery
19

20. BioIsoprene™ BioRefinery:
Market
Applications
Polymer Grade Cis 1,4
Tires /
BioIsoprene™ Polyisoprene
Elastomers
Product Synthetic Rubber
Replace Natural
Rubber
Renewable Gas-phase
Feedstocks BioIsoprene™ Synthetics in tires
product recovered
& purified to liquid-
phase
rDNA Host Non-tire synthetics
BioIsoprene™
produced in Systems:
gas-phase E.coli; Yeast; Fungi;
Adhesive
E • in-situ others Applications
purification
• relieves end
product
inhibition/toxicity
Other styrene
adhesives
• faster, better,
cheaper
20

21. BioIsoprene™ Molecule:
Genencor’s Unique C5 Platform
Tires
Biochemicals
Purification
Adhesives
High Purity
BioIsoprene™ monomer
Specialty Polymers
H CH3
C C
H2C CH2
Isoprene
Monomer Gasoline
Renewable Microbial Strain Large Scale Gas Phase
C10 Isomers
Feedstocks Development Fermentation Recovery
C15 Isomers
+
+ Other Fuels & Blends
Diesel
Biofuels BioIsoFuel™ Examples Jet Fuel
Chemical
Catalysis
21

22. C5 Platform Progress – Stage I
Biochemicals
Tires
BioIsoprene™ Project
Purification
Adhesives
High Purity
BioIsoprene™ monomer
Specialty Polymers
H CH3
C C
H2C CH2
Isoprene
Monomer
Microbial Strain Large Scale Gas Phase
Sugar
Development Fermentation Recovery
In Stage I development we have started from sugar
22

23. C5 Platform Progress – Stage II Biofuels
BioIsoFuel™ Products
Concerted effort converting biomass to sugar
• Leverage long history / IP with biomass enzymes
Continued optimization
Hybrid biological + chemical process
H CH3
C C
H2C CH2
Isoprene
Monomer Gasoline
Renewable Microbial Strain Large Scale Gas Phase
C10 Isomers
Feedstocks Development Fermentation Recovery
C15 Isomers
+
+ Other Fuels & Blends
Diesel
BioIsoFuel™ Exaamples Jet Fuel
Chemical
Partner Catalysis
23

24. Genencor®: on the Road to
Sustainable Biorefineries
The biobased economy is here and its growing
• ~5-10% of chemicals will be bio-based by 2012
(McKinsey 2006)
High oil prices and environmental concerns are key
factors fueling the biobased economy
Innovations in enzyme processing are enabling the
development of sustainable integrated systems for
biomass processing to fermentable sugars
New business models / Strategic relationships and
Technology Integrators will speed Biorefinery
development
Biobased processes will continue to deliver Strategic
alternatives to petrochemicals
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25. Our Vision of the Biobased Economy
We envision a future where biotechnology
fulfills many unmet needs…
 In development and deployment of
industrial enzymes
 In the production of fuels, chemicals and
materials
We envision a future where biotechnology
helps create sustainable industrial
activities
We envision a future where biorefineries
take their place alongside oil refineries
We aspire to be recognized as a leader in
world-changing technology solutions.
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26. Questions:
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