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Non-typhoidal Salmonellosis, is one of the most common and widely distributed foodborne diseases, with tens of millions of human cases occurring worldwide every year. In the United States, the incidence of NTS infection has doubled in the past 2 decades. In 2009 there were 14 million cases of NTS.
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INTRODUCTION The term ‘non-technical skills’ was first applied to safety by the European civil aviation regulator in relation to airline pilots’ behaviour on the flight deck but is now used by a number of professions . Non-technical skills can be defined as ‘ the cognitive, social, and personal resource skills that complement technical skills, and contribute to safe and efficient task performance. NTS typically include situation awareness, decision-making, team work, leadership, and the management of stress and fatigue. Safety & efficiency in any field of work is not just limited to possession of thorough academic knowledge & skilful application of the technical skills, but it also encompasses the basic human behaviour & attitude of individuals during the course of performance of their duties. Deficiencies in non-technical skills can increase the chances of error, which in turn can increase the chances of an adverse event. Detailed investigations of adverse health care events have shown that in almost 80% of the cases the underlying cause is poor application of NTS like poor communication, inadequate monitoring, failures to cross-check drugs and equipment. Good non-technical skills (e.g. vigilance, anticipation, clear communication, team coordination) can reduce the likelihood of error and consequently of accidents Two categories of NTS have been recognized: 1. Cognitive & Mental skills which include planning, decision making, situation awareness etc. 2. Social & Interpersonal skills like coordinated team work, leadership, communication etc.
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Infectious diseases are a major public health concern causing over 3.5 million deaths worldwide. Diagnosing patients as quickly and effectively as possible is crucial for managing disease outbreaks. Next-generation sequencing (NGS) provides unique capabilities to understand the genetic profile of infectious disease patients that no other technology can match. Whole-genome metagenomics allows clinicians to take a deeper dive into pathogens by generating big-data about their characteristics. This data can be rapidly analyzed using complex bioinformatics software algorithms to achieve clinical-grade diagnostic accuracy. In a healthcare system shifting towards personalized medicine, NGS can provide clinicians the tools that they need to prescribe individualized treatments to save patients who were previously untreatable. The result is improved quality of care, better treatment regimes, and cost-saving healthcare.
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Liquid biopsies enable us to monitor the evolution of genetic aberrations in primary tumors as they shed the tumor cells into the circulation. The limitation is the ability to detect these low frequency genetic aberrations in a consistent manner to understand short- and long-term implications and how this information will be used in the clinic. This slidedeck will cover the challenges and solutions associated with multiple steps as one starts with liquid biopsy and move towards finding a new biomarker.
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Antibiotic resistant strains of pathogenic bacteria are a growing worldwide health problem. To effectively combat the spread of difficult-to-treat bacterial infections, rapid surveillance methods for detection of antibiotic resistance genes is required to monitor both bacterial isolates and metagenomic samples. Additionally, identification of potential new sources for different antibiotic resistance genes is critical. Both of these goals require tools that can be used for profiling of antibiotic resistance genes from various types of samples. Real-time PCR has proven to be effective for the detection of antibiotic resistance genes. Using PCR array technology, simultaneous detection of 87 prevalent and important antibiotic resistance genes is possible and should prove to be an effective method for antibiotic resistance monitoring. This allows for a more comprehensive profiling of antibiotic resistance genes than is possible using individual PCR assays.
Identification of antibiotic resistance genes in Klebsiella pneumoniae isolat...
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Since its inception, next-generation sequencing has found utility in a diverse set of industries, from biomarker discovery in pharma to ancestral identification in archeology. Across the board, NGS has the advantage of allowing us to answer questions that require a lot of data. Next-generation sequencing provides orders of magnitude more data than traditional Sanger sequencing as hundreds of “lanes” analyzed in parallel vs. hundreds of millions of “clusters” which allows for many samples to be multiplexed on a single-run. By starting with different genetic material and following specific experimental workflows, NGS can be applied to many applications. Here we focus on DNA resequencing applications, which implies the data generated will be compared to an existing reference sequence (such as the human genome). Specifically, we’ll focus on how we can analyze patient-derived material to identify onco-relevant mutations including single-nucleotide variants, insertions-deletions, copy number variants and translocations. We’ll also focus on how known reference standards have been shown to be vital in ensuring data generated from NGS assays is accurate and reproducible.
Molecular QC: Using Reference Standards in NGS Pipelines
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Candy Smellie
Metagenomicsnewer approach in understanding Microbes by Dr.T.V.Rao MD
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QIAseq RNA is a revolutionary turnkey solution for digital gene expression analysis by NGS. From 10 genes to 1000, from one sample to 100, QIAseq RNA delivers precise results on both ION and Illumina sequencing platforms. The data from QIAseq RNA is directly comparable to expression analysis derived from whole transcriptome sequencing or by qRTPCR, only better, cheaper, faster, and more flexible. This webinar will describe the principles of digital expression analysis by NGS, and review the features and benefits of the QIAseq system, options available, and the integrated data analysis package.
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NGS for Infectious Disease Diagnostics: An Opportunity for Growth
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Making your science powerful : an introduction to NGS experimental design
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Step by Step, from Liquid Biopsy to a Genomic Biomarker: Liquid Biopsy Series...
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Computational infrastructure for NGS data analysis
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Discovery and Annotation of Novel Proteins from Rumen Gut Metagenomic Sequenc...
Discovery and Annotation of Novel Proteins from Rumen Gut Metagenomic Sequenc...
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Next Generation Sequencing of Fish Microbiome- AquaCyprus 2014
Next Generation Sequencing of Fish Microbiome- AquaCyprus 2014
Microbiome 2013
Microbiome 2013
Identification of antibiotic resistance genes in Klebsiella pneumoniae isolat...
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Molecular QC: Using Reference Standards in NGS Pipelines
Molecular QC: Using Reference Standards in NGS Pipelines
Metagenomics newer approach in understanding Microbes
Metagenomics newer approach in understanding Microbes
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Introduction to Metagenomics. Applications, Approaches and Tools (Bioinformat...
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