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EMBRACE – BioMart Developments & Future Syed Haider Rice Group - EBI July 2008
EMBRACE www.embracegrid.info European Model for Bioinformatics Research and Community Education ,[object Object],[object Object]
BioMart ,[object Object],[object Object],[object Object],www.biomart.org
BioMart ,[object Object],[object Object],[object Object],[object Object],[object Object]
In a nutshell ATGCTGTTGTGC ATGCTGGACTGG ATGGCCCGATGG ATGCTGTTGTGC ATGCTGGACTGG ATGGCCCGATGG Source data (MySQL, Oracle, Postgres) DB Mart
Deploying BioMart ,[object Object],[object Object]
1. Transformation ATGCTGTTGTGC ATGCTGGACTGG ATGGCCCGATGG ATGCTGTTGTGC ATGCTGGACTGG ATGGCCCGATGG DB Mart Source data (MySQL, Oracle, Postgres)
1. Transformation MartBuilder
2. Configuration Mart Mart Mart
2. Configuration MartEditor
User Interfaces
Concepts for End Users ,[object Object],[object Object],[object Object]
Examples ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Web Service Access <Query> <Dataset name=&quot; hsapiens_gene_ensembl &quot; > <Filter name=&quot; chromosome_name &quot; value=&quot; 1 &quot;/> <Attribute name=&quot; ensembl_gene_id &quot;/> <Attribute name=&quot; ensembl_transcript_id &quot;/> <Attribute name=&quot; biotype &quot;/> </Dataset> </Query> wget --post-data 'query= ‘ http://www.biomart.org/biomart/martservice
Web Service Access <Query> <Dataset name=&quot; hsapiens_gene_ensembl &quot; > <Filter name=&quot; chromosome_name &quot; value=&quot; 1 &quot;/> <Attribute name=&quot; ensembl_gene_id &quot;/> <Attribute name=&quot; ensembl_transcript_id &quot;/> <Attribute name=&quot; biotype &quot;/> </Dataset> </Query> wget --post-data 'query= ‘ http://www.biomart.org/biomart/martservice martview
VIRTUALSCHEMANAME=default &ATTRIBUTES = hsapiens_gene_ensembl .default.feature_page. ensembl_gene_id &FILTERS = hsapiens_gene_ensembl .default.filters. chromosome_name.&quot;1&quot; Web Service Access XML Free URL http://biomart.org/biomart/martview?
BioMart DAS Access http://www.YourBioMart.org/biomart/das/ DATASET /features? segment= FILTERS http://www.biomart.org/biomart/das/ default__hsapiens_gene_ensembl__ensembl_das_chr /features? segment= 1:1,100000 http://www.biomart.org/biomart/das/ default__hsapiens_gene_ensembl__ensembl_das_gene /features? segment= ENSG00000197194
Web based Access How far it has gone  ?
Taverna
Bioma R t - BioConductor package
Cytoscape
Galaxy
Template Queries
 
 
Learn as you go.... Show URL Request Show XML Query Show Perl Script
[object Object],[object Object],[object Object],[object Object],[object Object],Future
[object Object],[object Object],Future
Visualisation: Gene List Analysis & Clinical Significance ,[object Object],Gene List Visualisation Gene list analysis Clinical Significance
Map Genes onto Genome
Visualisation: Gene List Analysis & Clinical Significance ,[object Object],Gene List Visualisation Gene list analysis Clinical Significance
Map Genes onto GO GO Biological process (32) Cellular component (18) Molecular Function (24) Stem cell maintenance (7) Positive regulation of developmental process (8) Leukocyte mediated cytotoxicity (5) regulation of cell killing (12) Developmental process (15) Cell killing (17)
Visualisation: Gene List Analysis & Clinical Significance ,[object Object],Gene List Visualisation Gene list analysis Clinical Significance
Map Genes onto Pathways Reactome Apoptosis (43) Intrinsic pathway for apoptosis (26) Signaling by Wnt (10) Signaling by TGF β  (23)   Activation of BH3-only proteins (5) Permeabilization of mitochondria (3) Release of apoptotic factors from mitochondria (18)
Future ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Submission support
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Galaxy Credits References
[object Object]
BioMart Central Portal – queries served

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Haider Embrace Bosc2008

  • 1. EMBRACE – BioMart Developments & Future Syed Haider Rice Group - EBI July 2008
  • 2.
  • 3.
  • 4.
  • 5. In a nutshell ATGCTGTTGTGC ATGCTGGACTGG ATGGCCCGATGG ATGCTGTTGTGC ATGCTGGACTGG ATGGCCCGATGG Source data (MySQL, Oracle, Postgres) DB Mart
  • 6.
  • 7. 1. Transformation ATGCTGTTGTGC ATGCTGGACTGG ATGGCCCGATGG ATGCTGTTGTGC ATGCTGGACTGG ATGGCCCGATGG DB Mart Source data (MySQL, Oracle, Postgres)
  • 12.
  • 13.
  • 14. Web Service Access <Query> <Dataset name=&quot; hsapiens_gene_ensembl &quot; > <Filter name=&quot; chromosome_name &quot; value=&quot; 1 &quot;/> <Attribute name=&quot; ensembl_gene_id &quot;/> <Attribute name=&quot; ensembl_transcript_id &quot;/> <Attribute name=&quot; biotype &quot;/> </Dataset> </Query> wget --post-data 'query= ‘ http://www.biomart.org/biomart/martservice
  • 15. Web Service Access <Query> <Dataset name=&quot; hsapiens_gene_ensembl &quot; > <Filter name=&quot; chromosome_name &quot; value=&quot; 1 &quot;/> <Attribute name=&quot; ensembl_gene_id &quot;/> <Attribute name=&quot; ensembl_transcript_id &quot;/> <Attribute name=&quot; biotype &quot;/> </Dataset> </Query> wget --post-data 'query= ‘ http://www.biomart.org/biomart/martservice martview
  • 16. VIRTUALSCHEMANAME=default &ATTRIBUTES = hsapiens_gene_ensembl .default.feature_page. ensembl_gene_id &FILTERS = hsapiens_gene_ensembl .default.filters. chromosome_name.&quot;1&quot; Web Service Access XML Free URL http://biomart.org/biomart/martview?
  • 17. BioMart DAS Access http://www.YourBioMart.org/biomart/das/ DATASET /features? segment= FILTERS http://www.biomart.org/biomart/das/ default__hsapiens_gene_ensembl__ensembl_das_chr /features? segment= 1:1,100000 http://www.biomart.org/biomart/das/ default__hsapiens_gene_ensembl__ensembl_das_gene /features? segment= ENSG00000197194
  • 18. Web based Access How far it has gone ?
  • 20. Bioma R t - BioConductor package
  • 24.  
  • 25.  
  • 26. Learn as you go.... Show URL Request Show XML Query Show Perl Script
  • 27.
  • 28.
  • 29.
  • 30. Map Genes onto Genome
  • 31.
  • 32. Map Genes onto GO GO Biological process (32) Cellular component (18) Molecular Function (24) Stem cell maintenance (7) Positive regulation of developmental process (8) Leukocyte mediated cytotoxicity (5) regulation of cell killing (12) Developmental process (15) Cell killing (17)
  • 33.
  • 34. Map Genes onto Pathways Reactome Apoptosis (43) Intrinsic pathway for apoptosis (26) Signaling by Wnt (10) Signaling by TGF β (23) Activation of BH3-only proteins (5) Permeabilization of mitochondria (3) Release of apoptotic factors from mitochondria (18)
  • 35.
  • 36.
  • 37.
  • 38. BioMart Central Portal – queries served