SIRION BIOTECH Portfolio

1. Experts in Gene Silencing and Over-expression Delivering ANY Gene to ANY Cell

2. Sirion Offers Shorter Timelines and Improved Quality Custom development of genetically modified mammalian cells Cell line optimization services Cell line immortalization services

3. Sirion Delivers Shorter Timelines and Improved Quality Functionally tested, ready-to-use tailor-made genetically modified cells, Guaranteed gene delivery into virtually any mammalian cell type Guaranteed > 90% knockdown maintained over the required experimental time frame Results in weeks instead of the months needed for in-house solutions

5. Ready-to use frozen cells

8. Flexible choice of promoters and expression cassettes

9. Expression up to 4 genes in one vector possible

10. QC, RCA-tested vector preparationsHigh titer virus stocks for in vivo Your Benefits All vectors are guaranteed for function S1/BSL1 ready-to-use, pre-transduced, frozen cell stocks available Fast time lines ─ 4 weeks from gene to vector SIRION-Viral vectors SIRION BIOTECH State of the art transfection Molecular Myology, LMU Munich

12. Increased gene transfer in vivo

13. Accessibility of cell types MOI 200 MOI 200 NIH-3T3 cells were transduced with an Adenovirus Serotype 5 expressing EGFP with a MOI of 200 without and in the presence of TransMax.

15. Inducible knockdown via partnership with TET systemsshRNA design and cloning into Sirion screening system Determination of knockdown efficiency by QPCR Confirmation of knockdown on the protein level by Western blot Ad368-high performance shRNA >90% KD

16. >90% knockdown – Guaranteed! Shorter Timelines and Improved Quality High performance viral vectors with guaranteed >90% gene knockdown Stable or inducible knockdown cells for reliable results Short timelines save money compared to long in house development Sirion’s experienced team can quickly generate the cell model you need

18. Polyclonal or monoclonal cell line generation

20. Conditionally immortalized cells terminally differentiate with close to primary cell type phenotype and function for more reliable results.* hTERT requires a license from Geron Inc.

22. Guaranteed cell viability >90%

24. Drug candidate screening

25. Target validation in vivo (tumor models)

27. Identification ofcritical cellular pathways for productivity

28. Over-expression or knockdown of target genes

29. Stable genetic modification

30. Antibody yield (ELISA testing)

31. Virus yield (PFU/IU testing)

32. Cellular assays /enzyme activity

33. Culture medium optimization

34. Genetic cell modification

36. Experts in Gene Silencing and Over-expression Delivering ANY Gene to ANY Cell SIRION BIOTECH GmbH Tel: +49 89 700 961 999Am Klopferspitz 19 Fax: +49 89 700 961 99882152 Martinsried info@sirion-biotech.comGermany www.sirion-biotech.com