Li Huang, Montana State University

1. Novel resistance to rust pathogens in bread wheat
Li Huang
Montana State University

2. Mutagenesis
Alpowa--Susceptible line Leaf rust screening
Treat seeds with EMS Select for resistance ones
Mutation density: 1/12 kb (Feiz et al., 2009, Genetics)

3. Al-180 Al-220 Al-268 Al-271 Al-535
Alpowa MNs MNs MNs MNs MNs
APR
Pt S R R R R R
S R R R S S
Pgt
R R R R R S
Pst APR
S R R S S S

6. Al-220
‘Alpowa’ MNs

8. Infection types of the mutant and the wild type
with Pt race PBJL
3 dpi 6 dpi
Al-220 Al-220
‘Alpowa’ MNs ‘Alpowa’ MNs

9. Infection types of the mutant and the wild type
with Pt race PBJL at seedling stage
Al-220
‘Alpowa’ MNs
dpi 12 15 18 21 12 15 18 21

10. Infection types with Pt race PBJL
at adult stage
Al-220
‘Alpowa’ MNs
dpi 18 21 24 18 21 24

11. Infection types with Pt race PBJL
at seedling stage
Susceptible Resistance
8 11 14 17 20 8 11 14 17 20
Chinese Spring x MNsAl-220

12. Infection types with Pgt race TMLK
at seedling stage
Al-220
‘Alpowa’ MNs
dpi 18 21 24 18 21 24

13. Al-220
Infection types of MNs and ‘Alpowa’ with races
of three rust pathogens
Lines
Pathogens Races Stage Al-220
‘Alpowa’ MNs
PBJL Seedling 3~4 ;~2
Adult S R
Pt SBGD Adult S I
BBBDD Adult S I
78 Seedling 8 7
127 Seedling 8 8
Pst Adult R R
101/102 Adult S R
TLMK Seedling 3-~4 ;1+
QFCS 3~4 ;1+
TTKSK 3+ ;1+
TTKST 3+ ;1+
Pgt TTTSK 3+ ;
TPMK 4 ;
TTTT 4 ;1+
QTHJ 3+ ;1+

14. Infection types with a local Egt race
at seedling stage
Al-220
‘Alpowa’ MNs
dpi 18 21 24 18 21 24

15. Seedling stage
PR Genes Enzyme Actvity
PR1
PR2 β-1,3-Glucanase
PR3 Endochitinase
PR4 Endochitinase
PR5 Thaumatin-like
PR9 Lignin-forming Peroxidase
PR10 RNase
Adult stage

16. Al-220
MNs
‘Alpowa’

17. Al-220
Genetic analysis of resistance in the MNs
F2 individuals
Expected
Crosses Ratio X2
Resistant Susceptible
Al-220
MNs / ‘Alpowa’ 210 71 3:1 0.67
Al-220
‘Chinese Spring’ / MNs 100 36 3:1 0.69

18. 2BS
cM
Centromere
Genetics map of Lr48 Chinese Spring 2B Bin Map
Bansal et al., 2008, TAG Genetic map of MNsAL220 GrainGenes

19. Acknowledgement
The Huang Lab
Jackie Campbell
Dr. Hongtao Zhang
Collaborators
Dr. Mike Giroux MSU
Dr. Yue Jin USDA-ARS, Cereal Disease Lab
Dr. Meinan Wang WSU
Dr. Xianming Chen WSU
Dr. Deven See WSU
Funding