DR Anthony Crasto scaleup techniques & pilot plant, Glenmark scientist helping millions with websites, million hits on google amcrasto@gmail.com

1. Principal Scientist, Process Research A Short Presentation Dec 2011 amcrasto@gmail.com

7. Process flow

24. SRTM University, Nanded

27. Typical Distillation Pilot Plant Setup

28. DR ANTHONY CRASTO

30. Automated pilot plant , controlled with only one process control system for production of recombinant technical enzymes

34. Sugar Cane Crushing Cane Juice Sugar Process Molasses Bagasse Hydrolysate: Hexose, Pentose, Lignin Residues: Ash, Char Ethanol Fermentation Distillation Vinasse Biomass Hydrogen Fermentation Methane Fermentation Hydrogen Process E. Power Methane Process Steam A B C D 10,000 t/day (1 sugar Mill) 3.000 t/day (50% moisture) Hexose 525 t Pentose 315 t Lignin 210 t Residues 450 t Gas for industry Raw material Gas Fuel

35. Pilot Plant Placement

36. LAYOUT 01-5 100L Vessel 01-1-1Tank for raw material preparation 01-1-6 Stirring device 01-1-4 Stirring device

37. 01-3-1 Bioreactor 01-3-2 Circulation system 01-3-6 Flow monitor

39. Axial Compression Technology to semi-prep column (20 mm i. d. and 30 mm i. d.). The column bed is compressed adequately by attaching the end assembly newly designed. It provides proper bed density (10% higher than conventional columns) and bed uniformity. The combination of technology acquired by long our experience with DAC column, the advanced technique of slurry packing, and new hardware design offers an outstanding durability and efficiency Column : 50 X 20 mmI.D. 5 µm Eluent : A) water B) methanol Gradient : 5%B-95%B Flow rate : 50 mL/min Pressure : ∼ 17MPa

40. Conclusion The various process considerations described in this article can help chemists understand and adopt a systematic and prospective approach in research and development to have documented and controlled synthetic processes. This approach will help manufacturers meet product-quality objectives consistently and build a good basis for achieving the goals of prospective validation and scale-up activities.

42. https://sites.google.com/site/anthonycrastoscaleup/home