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BIO 101 Laboratory
                               This serves as a supplement in the laboratory.
                                    Don’t use this in your lecture class.


                                            EXERCISE VII
                                           PLANT TISSUES

A. MERISTIMATIC TISSUES / MERISTEMS
     composed of mitotically dividing cells
     responsible for elongation & widening the diameter of plant organs
     1. Apical Meristem
          found at the tips of stems (shoot apical meristem) & roots (root apical meristem); produce
              primary tissues
          account for primary growth → elongation of roots & shoots




           Root Apical Meristem                           Shoot Apical Meristem
                  (RAM)                                          (SAM)

     2. Intercalary Meristem
          occur between mature tissues
          most common in grasses → occur at the bases of the nodes
          help regenerate parts removed by grazing herbivores
          for rapid elongation of the internodes in monocots

     3. Lateral Meristem
          cylindrical meristems that form the subapical regions of the roots & shoots of woody plants
          produce secondary growth → increases the girth of the plant
          secondary growth makes the plant sturdier enabling it to grow taller & intercept light
          it includes the vascular cambium & cork cambium (also, phellogen)
               vascular cambium → forms the wood (secondary xylem) & secondary phloem
               cork cambium (phellogen → forms the periderm which in turn forms a layer of dead,
                                phellogen)
                                phellogen
               suberized cells that protects the inner tissues of the secondary plant body




                                                   -1-
BIO 101 Laboratory
                                This serves as a supplement in the laboratory.
                                     Don’t use this in your lecture class.




                  Intercalary Meristem                         Lateral Meristem
                (found only in monocots)            (Vascular cambium & Cork Cambium)

B. PROTECTIVE TISSUES / DERMAL TISSUES
     comprise the outermost layer of the plants
     1. Epidermis
          dermal tissue that covers the primary body of the plant, protecting the underlying tissues
          regulates the movement of gases between the plant and the atmosphere
          sheet of cutinized cells covering the plants to protect it against rapid water loss &
          mechanical injury
          modified epidermal cells:
              Guard Cells
                  kidney-shaped in dicots
                  dumbbell-shaped in monocots
              Trichomes
                  single-celled or multicellular outgrowths of epidermal cells
                  found in leaves, stems, flowers, & fruits
                  e.g.: cotton fibers → from the epidermis of cotton seeds
     2. Cork
          the outermost part of the periderm
          secondary tissue produced by the cork cambium




 Epidermis with Guard Cells &                   Trichomes                          Cork
           Stomata
           Stomata


                                                    -2-
BIO 101 Laboratory
                                This serves as a supplement in the laboratory.
                                     Don’t use this in your lecture class.

C. FUNDAMENTAL/ GROUND TISSUES
     more variable in function & type
     it constitute the mesophyll in leaves & the cortex & pith in stems & roots
     1. Parenchyma
           isodiametric or polyhedral cells with prominent protoplast & thin walls
           common examples are:
               Chlorenchyma – contains chloroplasts
               Aerenchyma – cells with prominent intercellular spaces
     2. Collenchyma
           consists of cells with unevenly thickened cell walls
           functions for support
     3. Sclerenchyma
           consists of cells which are devoid of protoplast upon maturity but has thick lignified walls
           two types of cells:
               Sclerids – short & polygonal
                  e.g.: stone cells in pears gives it a gritty texture (rough)
               Fibers – long and with tapering ends
                  e.g.: hemp fibers for making ropes

                      Parenchyma                      Collenchyma                      Sclerenchyma
Distinguishing    made up of                    made up of collenchyma           made up of fibers (long
   Features       parenchyma cells →            cells → elongated in             slender cells typically
                  usually many sided            shape                            occurring in strands) &
                                                                                 sclerids (variable shape,
                                                                                 shorter than fibers)
  Cell Wall       primary or secondary;         unevenly thickened               primary & thick secondary;
                  may be lignified,             primary cell wall only;          usually lignified
                  suberized, or cutinized       not lignified
                  alive at maturity             alive at maturity                maybe alive or dead at
                                                                                 maturity
 Location on      Throughout the plant          beneath the epidermis in         Fibers: usually associated
the Plant Body    body                          young elongating stems           with xylem & phloem; in
                                                often seen as a cylinder         leaves in monocots;
                                                of tissue or only in             sometimes in cortex of
                                                patches in ribs along            stems
                                                veins in some leaves             Sclerids: throughout the
                                                                                 plant
   Function       for metabolic processes       support in primary plant         Fibers → for support
                  such as respiration &         body                             Sclerids → for protection &
                  photosynthesis; storage                                        mechanical support
                  & conduction; wound
                  healing & regeneration




                                                    -3-
BIO 101 Laboratory
                                This serves as a supplement in the laboratory.
                                     Don’t use this in your lecture class.




       Parenchyma Cells                   Chollenchyma Cells                     Sclerenchyma Cells




                  Sclerenchyma (Fiber Cells)                  Sclerenchyma (Stone Cells)


D. Vascular / Conducting Tissues
     Vascular tissues are complex → i.e. composed of four types of cells
     1. Xylem
          for transport of water & dissolved minerals absorbed by the roots from the soil
          composed of fibers, parenchyma cells, vessels (large), & tracheids (small, devoid of
          protoplast)
             Vessels & Tracheids
                main water-conducting elements
                exhibit secondary wall thickenings which can be annular, spiral, scalariform,
                reticulate, or pitted




                             Xylem showing vessel elements & tracheids

                                                    -4-
BIO 101 Laboratory
                           This serves as a supplement in the laboratory.
                                Don’t use this in your lecture class.

                    in longitudinal (left) and transverse (right) sections.

2. Phloem
    for transport of food materials from the leaves to all plant parts
    in woody stems, phloem tissues constitute the greater bulk of the bark
    consists of fibers, parenchyma cells, & sieve tube cells (devoid of protoplast)
       Sieve Tube Cells
          main food-conducting elements
          thick-walled & provided at both ends with perforated sieve plates
       companion cells → associated with sieve tube cells




                  Phloem showing sieve tube members & companion cells
                     in longitudinal (left) & transverse (right) sections.




                                               -5-
BIO 101 Laboratory
           This serves as a supplement in the laboratory.
                Don’t use this in your lecture class.


                      PLANT ORGANS
           Vegetative Plant Organs:
           1. Roots
           2. Stems
           3. Leaves

           Reproductive Plant Organs:
           1. Flowers
           2. Fruits
           3. Seeds




A. ROOTS
                          Root System




                               -6-
BIO 101 Laboratory
                           This serves as a supplement in the laboratory.
                                Don’t use this in your lecture class.



                                         Monocot Root




                                      Young Eudicot Root




1. Epidermis – uncutinized cells with root hairs (to facilitate absorption)
2. Cortex – multi-layered parenchyma cells:
       Endodermis = inner most layer (endodermal cells)
       Casparian strip = suberized
       Passage cells = for water transport
3. Stele – innermost cylinder
       Pericycle – made up of parenchyma cells; gives rise to the vascular cambium &
       secondary/lateral roots
       Xylem – 3-4 radial rows of thick walled cells alternating with thin phloem cells
       Vascular cambium – meristimatic zone between phloem & xylem
       Phloem – thin-walled; food conducting cells alternating with xylem rays
       Pith – if present, composed of parenchyma cells


                                               -7-
BIO 101 Laboratory
           This serves as a supplement in the laboratory.
                Don’t use this in your lecture class.

B. Stems
                          Morphology




                Monocot stem (cross section)


                               -8-
BIO 101 Laboratory
                            This serves as a supplement in the laboratory.
                                 Don’t use this in your lecture class.



C. Leaf
               Morphology                                                    Venation




                                                         Monocot                      Eudicot
                                                        (Parallel)              (Netted/Reticulated)
     Monocot              Eudicot

                                            Simple Leaf




          Pinnately Compound Leaf                                 Palmately Compound Leaf




                                                -9-
BIO 101 Laboratory
                       This serves as a supplement in the laboratory.
                            Don’t use this in your lecture class.



                                   Leaf Arrangement




                                                                        Whorled
Alternate                           Opposite

            Monocot:




            Eudicot:




                                           - 10 -
BIO 101 Laboratory
                                 This serves as a supplement in the laboratory.
                                      Don’t use this in your lecture class.

D. Flower
                                                Morphology




            Complete Flower – has all 4 parts (sepals, petals, pistil, stamen)
            Incomplete – lacks one or more of the 4 parts
            Perfect Flower (bisexual) – has both stamens & carpels/ovary
            Imperfect Flower (unisexual)– has stamen or carpel but not both
                        Imperfect flower = Incomplete Flower
            Monoecious – male & female reproductive parts in separate flowers (e.g.: corn)
            Dioecious – male & female reproductive parts in separate plants (e.g.: papaya)

                                                Inflorescence




                                                     - 11 -
BIO 101 Laboratory
                                    This serves as a supplement in the laboratory.
                                         Don’t use this in your lecture class.

E. Fruit




           matured / ripened ovary
           holds & protects seeds
           Simple Fruit – developed from a single ovary
           Aggregate – several ovaries of a flower (sugar apple)
           Multiple – several ovaries of several flowers (pineapple)




TERMS:
    Pericarp - the fruit wall (derived from the ovary of the pistil) which is composed of the exocarp,
    mesocarp and endocarp (the three layers are not always recognizable, ex. dry fruit)
    Endocarp - the innermost layer of the pericarp (often hard, stony or papery)
    Mesocarp - the middle layer of the pericarp (often fleshy)
    Exocarp - the outermost layer of the pericarp (often like a skin or peel). (peach)


                                                        - 12 -
BIO 101 Laboratory
This serves as a supplement in the laboratory.
     Don’t use this in your lecture class.



              Types of Fruits

                   Fleshy




                    - 13 -
BIO 101 Laboratory
This serves as a supplement in the laboratory.
     Don’t use this in your lecture class.



                 Dry Fruits


              Dry Dehiscent




             Dry Indehiscent




                    - 14 -
BIO 101 Laboratory
          This serves as a supplement in the laboratory.
               Don’t use this in your lecture class.

F. Seed
            Albuminous (Endospermic) Seed :




                       (Non-
          Exalbuminous (Non-endospermic) Seed




                              - 15 -

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Plant tissues & organs

  • 1. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. EXERCISE VII PLANT TISSUES A. MERISTIMATIC TISSUES / MERISTEMS composed of mitotically dividing cells responsible for elongation & widening the diameter of plant organs 1. Apical Meristem found at the tips of stems (shoot apical meristem) & roots (root apical meristem); produce primary tissues account for primary growth → elongation of roots & shoots Root Apical Meristem Shoot Apical Meristem (RAM) (SAM) 2. Intercalary Meristem occur between mature tissues most common in grasses → occur at the bases of the nodes help regenerate parts removed by grazing herbivores for rapid elongation of the internodes in monocots 3. Lateral Meristem cylindrical meristems that form the subapical regions of the roots & shoots of woody plants produce secondary growth → increases the girth of the plant secondary growth makes the plant sturdier enabling it to grow taller & intercept light it includes the vascular cambium & cork cambium (also, phellogen) vascular cambium → forms the wood (secondary xylem) & secondary phloem cork cambium (phellogen → forms the periderm which in turn forms a layer of dead, phellogen) phellogen suberized cells that protects the inner tissues of the secondary plant body -1-
  • 2. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. Intercalary Meristem Lateral Meristem (found only in monocots) (Vascular cambium & Cork Cambium) B. PROTECTIVE TISSUES / DERMAL TISSUES comprise the outermost layer of the plants 1. Epidermis dermal tissue that covers the primary body of the plant, protecting the underlying tissues regulates the movement of gases between the plant and the atmosphere sheet of cutinized cells covering the plants to protect it against rapid water loss & mechanical injury modified epidermal cells: Guard Cells kidney-shaped in dicots dumbbell-shaped in monocots Trichomes single-celled or multicellular outgrowths of epidermal cells found in leaves, stems, flowers, & fruits e.g.: cotton fibers → from the epidermis of cotton seeds 2. Cork the outermost part of the periderm secondary tissue produced by the cork cambium Epidermis with Guard Cells & Trichomes Cork Stomata Stomata -2-
  • 3. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. C. FUNDAMENTAL/ GROUND TISSUES more variable in function & type it constitute the mesophyll in leaves & the cortex & pith in stems & roots 1. Parenchyma isodiametric or polyhedral cells with prominent protoplast & thin walls common examples are: Chlorenchyma – contains chloroplasts Aerenchyma – cells with prominent intercellular spaces 2. Collenchyma consists of cells with unevenly thickened cell walls functions for support 3. Sclerenchyma consists of cells which are devoid of protoplast upon maturity but has thick lignified walls two types of cells: Sclerids – short & polygonal e.g.: stone cells in pears gives it a gritty texture (rough) Fibers – long and with tapering ends e.g.: hemp fibers for making ropes Parenchyma Collenchyma Sclerenchyma Distinguishing made up of made up of collenchyma made up of fibers (long Features parenchyma cells → cells → elongated in slender cells typically usually many sided shape occurring in strands) & sclerids (variable shape, shorter than fibers) Cell Wall primary or secondary; unevenly thickened primary & thick secondary; may be lignified, primary cell wall only; usually lignified suberized, or cutinized not lignified alive at maturity alive at maturity maybe alive or dead at maturity Location on Throughout the plant beneath the epidermis in Fibers: usually associated the Plant Body body young elongating stems with xylem & phloem; in often seen as a cylinder leaves in monocots; of tissue or only in sometimes in cortex of patches in ribs along stems veins in some leaves Sclerids: throughout the plant Function for metabolic processes support in primary plant Fibers → for support such as respiration & body Sclerids → for protection & photosynthesis; storage mechanical support & conduction; wound healing & regeneration -3-
  • 4. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. Parenchyma Cells Chollenchyma Cells Sclerenchyma Cells Sclerenchyma (Fiber Cells) Sclerenchyma (Stone Cells) D. Vascular / Conducting Tissues Vascular tissues are complex → i.e. composed of four types of cells 1. Xylem for transport of water & dissolved minerals absorbed by the roots from the soil composed of fibers, parenchyma cells, vessels (large), & tracheids (small, devoid of protoplast) Vessels & Tracheids main water-conducting elements exhibit secondary wall thickenings which can be annular, spiral, scalariform, reticulate, or pitted Xylem showing vessel elements & tracheids -4-
  • 5. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. in longitudinal (left) and transverse (right) sections. 2. Phloem for transport of food materials from the leaves to all plant parts in woody stems, phloem tissues constitute the greater bulk of the bark consists of fibers, parenchyma cells, & sieve tube cells (devoid of protoplast) Sieve Tube Cells main food-conducting elements thick-walled & provided at both ends with perforated sieve plates companion cells → associated with sieve tube cells Phloem showing sieve tube members & companion cells in longitudinal (left) & transverse (right) sections. -5-
  • 6. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. PLANT ORGANS Vegetative Plant Organs: 1. Roots 2. Stems 3. Leaves Reproductive Plant Organs: 1. Flowers 2. Fruits 3. Seeds A. ROOTS Root System -6-
  • 7. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. Monocot Root Young Eudicot Root 1. Epidermis – uncutinized cells with root hairs (to facilitate absorption) 2. Cortex – multi-layered parenchyma cells: Endodermis = inner most layer (endodermal cells) Casparian strip = suberized Passage cells = for water transport 3. Stele – innermost cylinder Pericycle – made up of parenchyma cells; gives rise to the vascular cambium & secondary/lateral roots Xylem – 3-4 radial rows of thick walled cells alternating with thin phloem cells Vascular cambium – meristimatic zone between phloem & xylem Phloem – thin-walled; food conducting cells alternating with xylem rays Pith – if present, composed of parenchyma cells -7-
  • 8. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. B. Stems Morphology Monocot stem (cross section) -8-
  • 9. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. C. Leaf Morphology Venation Monocot Eudicot (Parallel) (Netted/Reticulated) Monocot Eudicot Simple Leaf Pinnately Compound Leaf Palmately Compound Leaf -9-
  • 10. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. Leaf Arrangement Whorled Alternate Opposite Monocot: Eudicot: - 10 -
  • 11. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. D. Flower Morphology Complete Flower – has all 4 parts (sepals, petals, pistil, stamen) Incomplete – lacks one or more of the 4 parts Perfect Flower (bisexual) – has both stamens & carpels/ovary Imperfect Flower (unisexual)– has stamen or carpel but not both Imperfect flower = Incomplete Flower Monoecious – male & female reproductive parts in separate flowers (e.g.: corn) Dioecious – male & female reproductive parts in separate plants (e.g.: papaya) Inflorescence - 11 -
  • 12. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. E. Fruit matured / ripened ovary holds & protects seeds Simple Fruit – developed from a single ovary Aggregate – several ovaries of a flower (sugar apple) Multiple – several ovaries of several flowers (pineapple) TERMS: Pericarp - the fruit wall (derived from the ovary of the pistil) which is composed of the exocarp, mesocarp and endocarp (the three layers are not always recognizable, ex. dry fruit) Endocarp - the innermost layer of the pericarp (often hard, stony or papery) Mesocarp - the middle layer of the pericarp (often fleshy) Exocarp - the outermost layer of the pericarp (often like a skin or peel). (peach) - 12 -
  • 13. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. Types of Fruits Fleshy - 13 -
  • 14. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. Dry Fruits Dry Dehiscent Dry Indehiscent - 14 -
  • 15. BIO 101 Laboratory This serves as a supplement in the laboratory. Don’t use this in your lecture class. F. Seed Albuminous (Endospermic) Seed : (Non- Exalbuminous (Non-endospermic) Seed - 15 -