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1. Bastiana/Yetti Hernaningsih J Clin Exp Hematopathol, Vol. 48, No.1, Apr 2008 1 Prediction of Clinical Outcome in Patients with Idiopathic Thrombocytopenic Purpura by Evaluating Bone Marrow Clot CD20+ B Lymphocytes and Morphological Changes of Megakaryocytes Departemen Patologi Klinik Universitas Airlangga/RSUD Dr. Soetomo 23 Februari 2010
2. Pendahuluan Journal Reading Bastiana, 2010 2 1 2 3 Idiopathic Thrombocytopenic Purpura Gangguan yang diperantarai imun Trombosit diopsonisasi oleh autoantibodi dan dihancurkan dini oleh RES Clinical outcome bervariasi 25 - 30% kronis dan menjadi refrakter terhadap kortikosteroid, splenoktomi, dan terapi lain Clinical outcome Add Your Text Faktor Prediktor belum diketahui
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4. Menentukan faktor mana yang dapat memprediksi outcome pada pasien ITP.Folikel hiperplastik dlm white pulp pd zona marginal, yang dibentuk oleh limfosit B gambaran patologis utama pada limpa Ditemukan ekspansi klonal sel B & Ab monoklonal anti CD20 berguna pada px ITP refrakter Gambaran patologis limfosit B masih belum jelas
5. Journal Reading Bastiana, 2010 4 Prognostik hasil terapi 13 minggu setelah terapi lengkap atau 6 bulan setelah diagnosis Perubahan patologis pada clot sumsum tulang (sutul) Tujuan Penelitian Pengaruh??
6. Journal Reading Bastiana, 2010 5 73 Pasien ITP (Trombosit <50x109/L) Jan 1997-Juni 2005 Clot / biopsi sutul BMA pd saat Dx Biopsi Sutul jika bahan BMA (-) Metode Penelitian Pemeriksaan Imuno- histokimia Dievaluasi blind oleh seorang ahli Patologi Dihubungkan dengan respon terapi Px dengan Myelodisplastic Syndrome pd sutul dieksklusi dari penelitian
7. Journal Reading Bastiana, 2010 6 Definisi respon terapi Metode Penelitian Complete response (CR) Jumlah trombosit minimal 150 x 109/L dalam 13 minggu setelah terapi lengkap. Relaps Jumlah trombosit yang menurun <50x109/L sesudah mencapai CR atau PR Partial response (PR) Peningkatan jumlah dua kali dari jumlah trombosit awal atau jumlah trombosit di atas 50 x 109/L. Refrakter Pasien yang tidak dapat mencapai CR atau PR dengan terapi pertama dan atau terapi kedua
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9. Metode Penelitian Journal Reading Bastiana, 2010 8 Pemeriksaan Imunohistokimia Clot sumsum tulang difiksasi formalin diletakkan dalam parafin Pewarnaan dengan hematoxylineosin 1 3a 2000.10 Add Your Text 2000.10 Add Your Text 2000.10 Add Your Text 3b 2 Pewarnaan dobel dengan metode naphthol-ASD chloro-acetate esterase plus Giemsa (ASD-Giemsa method). 2003.10 Add Your Text 2003.10 Add Your Text 2003.10 Add Your Text Pemotongan serial 2001.10 Add Your Text 2001.10 Add Your Text 2001.10 Add Your Text
10. Studi Imunohistokimia: metode streptavidin-biotin. Antibodi monoklonal tikus, termasuk CD20 (Dako, Glostrup, Denmark) sebagai antibodi primer. Antigen retrieval dilakukan dengan 0.5 % trypsin digestion pada 37°C selama 30 menit atau dengan microwave dalam 0.1 M citrate buffer pada pH 6.0. Aktivitas peroksidase endogen dihambat melalui inkubasi dengan0.3% H2O2 dalam methanol. Reaksi dibuat visibel dengan 3,3’diaminobenzidinedalam 0.005% H2O2sebagai chromogen, diikuti dengan counterstaining dengan Mayer hematoxylin. Journal Reading Bastiana, 2010 9 Metode Penelitian Metode Streptavidin-Biotin
11. Fisher’s exact: analisis data kategori. One-way Anova: Analisis distribusi variabel kontinyu: analisis satu arah (one-way analysis) Kruskal-Wallis test (jika variasi tidak homogen) Multivariate logistic regression models: (menghitung odds ratio faktor resiko potensial dari outcome Journal Reading Bastiana, 2010 10 Analisis Statistik Nilai p < 0.05 ada perbedaansignifikan Semua analisis dilakukan menggunakanSPSS 11
12. Clot sutul yang diperoleh saat diagnosis berisi sel berinti yang memadai untuk pemeriksaan patologis pada 56 dari 73 pasien. Median umur 56 pasien: 66 tahun (16-86 tahun). 25 laki-laki dan 31 perempuan. Median durasi dari follow-up: 15,6 bulan (0,03-139,8 bulan). Karakteristik pasien: tabel 1. Temuan patologis: gambar 1. Journal Reading Bastiana, 2010 11 Hasil: Karakteristik Pasien
19. tidak ada peningkatan CD20+ limfosit (< 1% dari sel berinti) Berdasarkan temuan patologis pada clot sutul, peneliti menggolongkan pasien dalam 3 kelompok:
20. Journal Reading Bastiana, 2010 14 Gambar 1 Temuan patologi pada ITP 3 kelompok Pasien (tabel 1): Kelompok A (n = 21); B (n = 21) dan C (n = 14)
21. Journal Reading Bastiana, 2010 15 Gambar 1 Temuan patologi pada ITP Gambar (1a) Temuan histopatologis sutul dari kasus ITP. Peningkatan jumlah megakariosit secara random terdistribusi pada partikel sutul. (clot sutul, pengecatan HE , x20). Gambar (1b) Megakariosit dari kasus ITP. Megakariosit matur menunjukkan pinggiran sitoplasma yang tebal, proyeksi sitoplasma seperti “corona” dengan perlekatan neutrofil pada puncak proyeksi. Tidak ada temuan displastik. (clot sutul, clot, pengecatan HE, x400). Immunohistological analysis of bone marrow clots
22. Journal Reading Bastiana, 2010 16 Gambar 1 Temuan patologi pada kasus ITP Gambar (1c) Temuan sutul pada kasus ITP. Sekitar 5% sel yang diperiksa dengan lapangan pandang besar adalah CD20+ B-cells (clot sutul, CD20 immunostaining, x100). Gambar (1d) Temuan sutul dari kontrol normal. Kurang dari 1 % sel adalah CD20+ B-cells. (clot sutul, CD20 immunostaining, x200).
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24. Hasil dari 13C urea breath test dan eradikasi H. pyloria ditunjukkan pada tabel 1.
26. Hasil Penelitian: Temuan Patologis dan Respon TerapiHasil analisis multivarian ditunjukkan pada tabel 2. Temuan patologis merupakan faktor prognostik yang signifikan terhadap respon terapi. Dibandingkan dengan kelompok A, kelompok B merupakan faktor prognostik yang signifikan untuk mencapai CR, (p = 0.04; odds ratio, 6.65; 95% confidence interval, 1,09 - 40,54) Status kelompok C merupakan faktor prognostik yang signifikan untuk respon terapi apapun (p = 0.04; odds ratio, 14.26; 95% confidence interval, 1,08 – 188,02). Journal Reading Bastiana, 2010 19
28. 3 pasien yang refrakter terhadap terapi apapun, semuanya termasuk kelompok A. Satu dari 3 pasien meninggal karena pneumonia cytomegalovirus 18 bulan setelah diagnosis. Dua pasien lainnya diobati secara suportif dan masing-masing bertahan hidup selama 36 dan 90 bulan setelah diagnosis. Journal Reading Bastiana, 2010 21
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30. Pasien kelompok A (termasuk semua pasien refrakter) menunjukkan treatment outcome paling jelek.
31. Hal tsb menunjukkan adanya CD20+ limfosit pada sutul berhubungan dengan outcome yang jelek .Journal Reading Bastiana, 2010 22
32. Diskusi Peneliti menekankan pentingnya investigasi dari B cell clones. Penelitian sebelumnya menunjukkan B-cell clones pada ITP mungkin memproduksi antibodi anti platelet dan terlibat dalam aktivasi fungsi reseptor Fc makrofag dan klirens trombosit yang ter-coated IgG. Namun peran B cells masih kontroversial. Peningkatan B-cell clones mungkin merupakan hasil dari disregulasi sel B. Penelitian lebih lanjut peran clones tersebut dapat memungkinkan interpretasi lebih baik dari temuan ini. Journal Reading Bastiana, 2010 23
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34. Perlekatan antibodi anti platelet dan komplemen ke megakariosit dapat meginduksi perubahan tersebut.
35. Diperlukan penelitian lebih lanjut untuk menentukan patofisiologi yang tepat.Journal Reading Bastiana, 2010 24
36. Median umur tinggi dan dominasi pasien bukan perempuan tidak konsisten dengan penelitian ITP sebelumnya pada orang dewasa. Latar belakang pasien mungkin mempengaruhi hasil, sehingga perlu penelitian skala besar. Journal Reading Bastiana, 2010 25
37. Penelitian terbaru menunjukkan ada hubungan antara infeksi H. pylori dengan ITP. Pada penelitian ini, adanya infeksi H. pylori infection hampir sama pada semua kelompok. Namun jumlah pasien yang menjalani 13 C urea breath test kecil. Journal Reading Bastiana, 2010 26
38. Penelitian ini memberikan informasi terbaru (novel) pengembangan stratetegi berbasis resiko (risk based strategy) dalam mengobati ITP. Penelitian ini memiliki keterbatasan: - jumlah pasien sedikit - retrospektif: ada bias mempengaruhi hasil. - Heterogeneitas antara spesimen berbeda dari pasien yang sama, tidak diteliti. - Observer sampel sutul hanya satu variasi inter observer tidak diketahui. Journal Reading Bastiana, 2010 27
41. Penelitian hubungan infeksi H.pylori dengan temuan imunohistopatologis clot sutul juga diperlukan.Journal Reading Bastiana, 2010 28
42. Pasien dengan peningkatan CD20+ lymphocytes dan perubahan morfologi megakaryocytes, lebih muda usianya daripada pasien dengan perubahan patologis yang lebih sedikit. Hal tsb. menunjukkan bahwa etiologi dan manejemen optimal ITP mungkin berbeda antara pasien yang lebih muda dengan yang lebih tua. Perlu penelitian multisentris dengan skala besar untuk mendapatkan interpretasi yang akurat. Journal Reading Bastiana, 2010 29
43. Kesimpulan Pendekatan berdasarkan pemeriksaan patologis tampaknya berguna untuk menentukan strategi berbasis resiko, dalam pengobatan pasien ITP. (Pasien ITP dapat diklasifikasi dengan clinical outcome yang berbeda berdasarkan pemeriksaan imununohistopatologis clot sumsum tulang). Journal Reading Bastiana, 2010 30
44. 31 Thank You ! Journal Reading Hematology II, 23 Februari 2010 Bastiana/Yetti Hernaningsih
45. How might H pylori infection contribute to the development of thrombocytopenia? H pylori expresses Lewis (Le) antigens in a strain-specific manner; Le antigens adsorb to platelets and might serve as targets for anti-Le antibodies in patients with an appropriate genetic background. In addition, both H pylori infection and ITP are associated with a T helper 1 (Th1)–type immune response characterized by increased levels of interferon and interleukin-2; hence, H pylori–induced alterations in cytokine profiles might promote development of immune thrombocytopenia Journal Reading Bastiana, 2010 32
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47. This interaction might contribute to the association of H pylori with cardiovascular disease and promote platelet consumption.
48. Finally, direct antigen mimicry between H pylori and platelet glycoproteins must be considered.Journal Reading Bastiana, 2010 33
49. Standard Immunohistochemistry Staining MethodLabeled Streptavidin Biotin (LSAB) Method Paraffin section or frozen section to water and rinse in PBS-Tween 20 for 2x2 min. Antigen Retrieval: perform antigen retrieval using IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) or IHC-TekTM Proteinase K Solution (Cat# IW-1101)if necessary. Serum Blocking: incubate sections in normal serum – species same as secondary antibody. Note: since this protocol uses avidin-biotin detection system, avidin/biotin block may be needed based on tissue type. Primary Antibody: incubate sections with primary antibody at appropriate dilution in IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) for 1 hour at room temperature or overnight. No serum blocking is needed if antibody diluent is used. Rinse in PBS-Tween 20 for 3x2 min. Peroxidase Blocking: incubate sections in peroxidase blocking solution for 10 minutes at room temperature. Note: For acetone fixed frozen sections, perform this peroxidase blocking step using 0.3% H2O2 in methanol prior to primary antibody incubation to avoid tissue distruction. Rinse in PBS-Tween 20 for 3x2 min. Secondary Antibody: incubate sections in Biotinylated secondary antibody in PBS for 30 minutes at room temperature. Rinse in PBS-Tween 20 for 3x2 min. Detection: incubate sections in HRP-Streptavidin solution for 30 minutes at room temperature. Rinse in PBS-Tween 20 for 3x2 min. Chromagen/Substrate: incubate sections in peroxidase substrate solution. Rinse in PBS-Tween 20 for 3x2 min. Counterstain with counterstain solution if desired. Rinse in running tap water for 2-5 minutes. Dehydrate through 95% ethanol for 1 minute, 100% ethanol for 2x3min. Clear in xylene for 2x5min. Coverslip with mounting medium. Journal Reading Bastiana, 2010 34
50. Blood. 2001; 98:952-957 Novel approaches to treatment of chronic refractory ITP are based on a better understanding of the immunologic abnormalities associated with this disorder, as well as the availability of sophisticated biotechnology products. One such approach involves the use of monoclonal antibodies directed against the CD20 antigen, a transmembrane protein found on the surface of normal and malignant B cells, but not on hematopoietic stem cells, pro-B cells, normal plasma cells, or other normal tissues. The CD20 molecule appears particularly suitable for targeted therapy because it does not shed from the cell surface and does not internalize upon antibody binding. Journal Reading Bastiana, 2010 35
51. Blood. 2001; 98:952-957 Rituximab is a genetically engineered chimeric murine/human anti-CD20 monoclonal antibody currently used for the treatment of non-Hodgkin lymphoma. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. The Fab domain of rituximab binds to the CD20 antigen on B lymphocytes, and the Fc domain recruits immune effector functions to mediate B-cell lysis in vitro. Possible mechanisms of cell lysis include complement-dependent cytotoxicity, antibody-dependent cellular cytotoxicity, and induction of apoptosis. Rituximab is highly effective for in vivo depletion of B cells, and the rationale for its use in chronic ITP rests on the attempt to eliminate autoreactive B-cell clones. Journal Reading Bastiana, 2010 36
52. Platelets: In General normal platelet count in adults ranges from 150,000-450,000/microL Surgical bleeding usually does not occur until the platelet count is less than 50,000, and spontaneous bleeding does not occur until the platelet count is less than 10,000-20,000 Journal Reading Bastiana, 2010 37
53. Platelets: In General Platelets are produced in bone marrow from Megakaryocytes An estimated 1000-5000 platelets are produced from each Megakaryocyte In normal adults platelet production is ~35,000-50,000/microL of whole blood per day. This value can be increased 8-fold during times of increased demand Journal Reading Bastiana, 2010 38 www.UpToDate.com 2005
54. Mechanisms of Thrombocytopenia Decreased platelet production Increased platelet destruction Dilutional Thrombocytopenia Splenomegaly or splenic sequestration Pseudothrombocytopenia Journal Reading Bastiana, 2010 39
55. Decreased Platelet Production Usually some offense that causes bone marrow suppression or damage Viral illness HIV (direct damage to Megakaryocytes) Chemo- or radiation therapy Congenital or acquired bone marrow aplasia or hypoplasia Direct EtOH toxicity Vit. B12 or Folate deficiency Journal Reading Bastiana, 2010 40
57. Splenic Sequestration Normally, ~1/3 of platelets are sequestered in the spleen in any given time In extreme splenomegaly, up to 90% of platelets can be trapped in the spleen Platelet size and life span is usually not affected Cirrhosis, Portal HTN, splenomegaly can all present with apparent thrombocytopenia, although these pts are not usually at risk for clinical bleeding Journal Reading Bastiana, 2010 42
60. Initial Approach H&P Bleeding Hx Drug Ingestion CBC and peripheral smear—”gold standard” Bone Marrow aspiration and biopsy—indicated if no other etiology obvious unless pt is <60yrs old. The presumptive Dx in that case is usually ITP. Idiopathic Thrombocytopenic Purpura (ITP) is a Dx of exclusion when H&P,Drug ingestion,CBC, and peripheral smear do not suggest other etiologies Journal Reading Bastiana, 2010 45
62. IMUNOHISTOCHEMISTRY Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues and consists of the following steps: 1)primary antibody binds to specific antigen; 2) antibody-antigen complex is bound by a secondary, enzyme-conjugated, antibody; 3) in the presence of substrate and chromogen, the enzyme forms a colored deposit at the sites of antibody-antigen binding. Journal Reading Bastiana, 2010 47
63. Humoral Immunity refers to the production of antibody molecules in response to an antigen (def). These antibody molecules circulate in the blood and enter the tissue via inflammation. Humoral immunity is most effective against bacteria, bacterial toxins, and viruses prior to these agents entering cells. Antibodies or immunoglobulins (def) are specific glycoprotein configurations produced by B-lymphocytes and plasma cells in response to a specific antigen and capable of reacting with that antigen. Journal Reading Bastiana, 2010 48
64. The antibodies produced during humoral immunity ultimately defend the body through a variety of different means These include:1. Opsonization2. MAC Cytolysis3. Antibody-dependent Cellular Cytotoxicity (ADCC) by NK Cells4. Neutralization of Exotoxins5. Neutralization of Viruses6. Preventing Bacterial Adherence to Host Cells7. Agglutination of Microorganisms8. Immobilization of Bacteria and Protozoans. Journal Reading Bastiana, 2010 49
65. Antibody-Dependent Cellular Cytotoxicity (ADCC) by NK cells NK cells are capable of antibody-dependent cellular cytotoxicity or ADCC. NK cells (def) have receptors on their surface for the Fc portion of IgG. When the antibody (def) IgG is made against epitopes (def) on "foreign" membrane-bound cells, e.g., virus-infected cells and cancer cells, the Fab portions (def) of the antibodies react with the "foreign" cell. The NK cells then bind to the Fc portion (def) of the antibody (see Fig. 1). Journal Reading Bastiana, 2010 50
66. The NK cell then releases pore-forming proteins called perforins, proteolytic enzymes called granzymes, and chemokines Granzymes pass through the pores and activate the enzymes that lead to apoptosis (def) of the infected cell by means of destruction of its structural cytoskeleton proteins and by chromosomal degradation (see Fig. 1A and Fig. 2). As a result, the cell breaks into fragments that are subsequently removed by phagocytes. Perforins can also sometimes result in cell lysis. (When NK cells are carrying out ADCC, they are sometimes also referred to as killer cells.) Journal Reading Bastiana, 2010 51
78. Hematoxylin and Eosin (H&E) staining •Place slides containing paraffin sections in a slide holder (glass or metal) •Deparaffinize and rehydrate sections: 3 x 3´ Xylene (blot excess xylene before going into ethanol) 3 x 3´ 100% ethanol 1 x 3´ 95% ethanol 1 x 3´ 80% ethanol 1 x 5´ deionized H2O •While sections are in water, skim surface of hematoxalin with a Kimwipe to remove oxidized particles. Blot excess water from slide holder before going into hematoxalin. •Hematoxalin staining: 1 x 3´ Hematoxalin Rinse deionized water 1 x 5´ Tap water (to allow stain to develop) Dip 8-12x (fast) Acid ethanol (to destain) Rinse 2 x 1´ Tap water Rinse 1 x 2´ Deionized water (can leave overnight at this stage) •Blot excess water from slide holder before going into eosin. •Eosin staining and dehydration:1 x 30 sec Eosin (up to 45 seconds for an older batch of eosin) 3 x 5´ 95% ethanol3 x 5´ 100% ethanol (blot excess ethanol before going into xylene) 3 x 15´ Xylene •You can leave slides in xylene overnight to get good clearing of any water. •Coverslip slides using Permount (xylene based). •Place a drop of Permount on the slide using a glass rod, taking care to leave no bubbles. •Angle the coverslip and let fall gently onto the slide. Allow the Permount to spread beneath the coverslip, covering all the tissue. •Dry overnight in the hood. Journal Reading Bastiana, 2010 54
80. ITP 100 cases per 1 milion persons per year. About half of these cases occur in children. Primary vs Secondary . Acute vs Chronic ( >6 months). Journal Reading Bastiana, 2010 56
81. The etiology is still unknown and the pathogenesis is complex and possibly depends on disturbed antigen presentation, T cell activation and signaling, disregulated B cell stimulation and antibodies, unbalanced activation / suppression of complement. Journal Reading Bastiana, 2010 57
82. Affected children are young (peak age ~ 5yrs) and previously healthy, and they typically present with the sudden onset of petechiae or purpura a few days or weeks after an infectious illness. Boys and girls are equally affected. Journal Reading Bastiana, 2010 58
87. Pathophysiology ITP is mediated by IgG autoantibodies. Glycoprotein IIb/IIa, Ib/Ix, Ia/IIa, IV and V ... Accelerated clearance through Fcү receptors that are expressed by tissue macrophages (spleen & liver). Journal Reading Bastiana, 2010 61
89. Genetics Monozygotic twins. An increased prevalence of HLA-DRw2 and DRB1*0410 alleles. HLA-DR4 and DRB1*0410 alleles have been associated with unfavorable and favorable response to corticosteroids, respectively. Journal Reading Bastiana, 2010 63
90. Diagnosis The diagnosis of ITP remains one of exclusion. Secondary forms of the disease occur in association with SLE, the antiphospholipid syndrome, immunodeficiency states (IgA deficiency and common variable hypogammaglobulinemia), Lymphoproliferative disorders (CLL, Large granular lymphocytic leukemia, and lymphoma), infection with HIV and hepatitis c virus, and therapy with drugs such as heparin and quinidine. Journal Reading Bastiana, 2010 64
91. The guidelines of the American Society of Hematology state that a bone marrow examination is not required in adults younger than 60 yrs of age if the presentation is typical but is appropriate before splenectomy is performed. Journal Reading Bastiana, 2010 65
92. Marrow examination is necessary in the presence of atypical features (e.g., those with additional cytopenias, protracted fever, bone or joint pain, unexplained macrocytosis ), or in patients who do not have a brisk or robust response to therapy. Journal Reading Bastiana, 2010 66
93. There is consensus, that bone marrow examination is not necessary in children if management involves observation or IVIG. Although it is not mandatory, many pediatric hematologists recommend that an aspiration be performed before starting corticosteroids to rule out the rare case of acute leukemia. Journal Reading Bastiana, 2010 67
94. The direct assay for the measurement of platelet-bound AB’s has an estimated sensitivity of 49-66%, an estimated specificity of 78-92%, and an estimated PPV of 80-83%. Journal Reading Bastiana, 2010 68
97. Treatment Watch & Wait strategy. Corticosteroids (high, standard or low dose). IVIG (high or low dose, 2 day or 1 day). IV anti-D immunoglobulin in Rh(D) positive patients (high or low dose). Journal Reading Bastiana, 2010 71