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Objective(s): There is a rising use of gold nanoparticles (AuNPs) in goods and in the medical fields but there is concern about the toxicity of them. So in this study spherical AuNPs with 3 different concentrations were applied for investigating their effects in vivo. Materials and Methods: 40 male albino mice were randomly divided into sham, control, 25 ppm, 50 ppm, 100 ppm groups and were treated by intraperitoneal injection for period of 14 days. Blood was taken for measuring of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase (SGOT and SGPT) enzyme levels and Complete Blood Count (CBC). Results: After the treatment and comparing groups with sham group, in 50 ppm group significant increases on RBC, HCT, HGB, MCHC and in 25 ppm group significant increase on MCHC and significant decrease on MCV and in 100 ppm group significant increase on MCHC were observed. Also in 50 ppm group an increase on SGOT enzyme level was observed. However, it was nonsignificant. Conclusion: By observing the abnormality on the RBC count and SGOT enzyme level in the 50 ppm group, we concluded a slight toxicity effect for AuNPs and the threat potential of their use in human.

Effects of intraperitoneal injection of gold nanoparticles in male mice

Nanomedicine Journal (NMJ)

Working class family

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Objective(s): This study aimed to address the gold nanoparticle(GNP)-dose and exposure duration effect on the kidney function of rats: in vivo. Materials and Methods: A total of 32 healthy male Wistar rats were used in this study. Animals were randomly divided into groups, three GNP-treated groups and control group. Group 1, 2 and 3 received. /5 cc of solution containing 5, 10,100 ppm Au via IP injection for 7 successive days, respectively. The control group was treated with 0.5% normal saline. Several biochemical parameters such as BUN (blood urea nitrogen), creatine and uric acid were evaluated at various time points (7 and 14 days). After 14 days, the tissue of kidney was collected and investigated. Results: There was no significant difference between the control and the intervention group regarding the amount of creatine-BUN and uric acid. The amount of creatine-BUN and uric acid showed increase in all the groups [except group1 (creatine) and group 2 (uric acid)] in the 7 and 14 days after intervention compared to the control group, but this difference was not significant. Results of histopatological tissue kidney showed: in group 1 and 3, complete destruction of the proximal tubules and distal cortical, in group 2, almost complete destruction of proximal tubules and distal. Conclusions: The induced histological alterations might be an indication of injured renal tubules due to GNPs toxicity that become unable to deal with the accumulated residues resulting from metabolic and structural disturbances caused by these NPs

The effect of gold nanoparticle on renal function in rats

Nanomedicine Journal (NMJ)

Liberalisme i nacionalisme

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Discussion: Can ‘agile software development’ be refactored to ‘agile product development’? Some brave pioneers already doing this are re-learning that building good product is more opaque than simply getting work done. The land of product development is filled with holes, ambiguity and landmines of wrongness. Ideas that you are stone certain about often fizzle or change when you watch someone interact with your product. Being overly certain or focusing on ‘just getting work done’ to sustain velocity are mistakes that make matters worse. Join me in an exploration of how to embrace wrongness, learn from it, and make it a vital part of our success. Our journey will explore the messy, sloppy and non-linear aspects of product development. Along the way, we’ll investigate how software construction is important, but courageously failing and learning in product is even more essential. We’ll look at how some teams are producing more real product value with less code. We will also peer into the world of program level development, where collections of teams produce better product by employing what might be called ‘test driven product.’ Who knows, toward the end of the journey, we might even rally to refactor the agile manifesto to read ‘Learning in Product over Simply Getting Things Done.’

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Objective(s): Vinblastine is antimitotic, anticancer medicine that disturbs normal microtubule formation and favours depolymerisation. Structural study and finding the active site of vinblastine are the targets of this research. Materials and Methods: Vinblastine was optimized in vacuum and then in different solvents by Density Functional Theory (DFT) method. Nuclear Magnetic Resonance (NMR) shift measurements were made in different solvents by various dielectric constants by Continuous Set of Gauge Transformations (CSGT). Results: The best structure and function of vinblastine was established. The conformational preferences may be attributed to stereoelectronic effects. The results showed that the structure of vinblastine is more stabile in water rather than the other media. The most active atoms of vinblastine were realized by various spectra of vinblastine in different media including vacuum and diverse solvents. Conclusion: Discovery of active site of vinblastine that could bind to tubulin to perform the antimitosis and anticancer effect in process of cell division was accomplished in this investigation. These data can be applicable to study the binding site of vinblastine-tubulin complex.

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