Metagenómica y sus Aplicaciones Industriales

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[object Object],[object Object],[object Object],[object Object],[object Object],Utilizan alrededor del 75% de las enzimas producidas industrialmente.

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Frozen stock, E.coli EPI 300 Streaked on LB-agar+Chloramp. plate, 37°C, 5 days. LB-broth+Chloramp. test tube, 37°C, 4.5hr, 225(xg). 250-mL LB-broth, 37°C, 225(xg) overnight. 20-L seed bioreactor 1500-L bioreactor, 110 (xg), 37°C,24hr.

Disk centrifuge, 9,000(xg), 25°C, 30min. High-speed blender Microfluidizer 1,600psig, 4°C Tangential-flow-filter-unit

Centrifuge, 4°C, 30min . Ultrafiltration DEAE-Sephadex column (anion exchange chromatography)

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