Teflabs introduces its family of next generation ion indicator dyes - Asante Ca Red visible ratiometric 488 nM excitation, dual emission at 525 and 650, 310 Kd.

1. Empowering Research

2. Teflabs is proud to
announce its new family of
fluorescent Calcium ion
indicator dyes

3. Identity Presentation: ASANTE SYMBOL
This mark was derived from the Ghanaian symbol for “except for God.” This version can also represent a
cell or strains of DNA with the smaller spot representing the Asante dye Integrating traditional
DNA, dye.
This mark was derived from the Ghanaian symbol for “except for God.” This version can also represent a
symbolism into the contemporary design also helps to add depth to the meaning and origin of the name
Next Generation Ion Indicator Dyes
“Asante.”
cell or strains of DNA with the smaller spot representing the Asante dye Integrating traditional
DNA, dye.
symbolism into the contemporary design also helps to add depth to the meaning and origin of the name
“Asante.”

5. Asante Ca2+ Red TM
Visible. Red. Ratiometric.

6. in vitro titration results
Asante Calcium Red (ex 488 nm, Kd ~310 nM)
900000
intensity (cps)
600000
300000
0
500 550 600 650 700
wavelength (nm)
0 nM Ca2+ 50 nM Ca2+ 150 nM Ca2+ 450 nM Ca2+
39000 nM Ca2+

8. in vivo test results

9. Loading of rat cardiac myocytes
Acutely dissociated rat cardiac myocytes were incubated for 45 min at room
temperature with Tyrode’s solution containing 10 µM Asante Calcium Red
AM and 0.02% (wt/vol) Pluronic F-127. The cells were then transferred to
fresh Tyrode’s solution and allowed to stand for an additional 45 min to
ensure complete hydrolysis of the AM ester.

10. Confocal fluorescence imaging
Myocytes loaded with Asante Calcium Red were superfused with Tyrode’
solution and imaged on a Zeiss 5 Live laser scanning confocal microscope.
With 488-nm excitation, fluorescence images were acquired at 525 nm and
650 nm at 19 frames per second. The background fluorescence for each
image was defined as the mean intensity value in a region-of-interest located
near the cell but outside the cell contour. After background correction and
smoothing, at each time point, the 650-nm image was divided by the 525-
nm image to generate the ratio image. To evoke a Ca 2+ transient and
contraction, the cell was stimulated with an electric field pulse delivered
through platinum electrodes immersed in the Tyrode’s solution.

11. Time (sec)
1.501
1.447 1.501 1.552 1.447 1.552
2000
1500
F650 nm
F650
1000
500
250
200
F525 nm
150
F525
100
50
0
20
650/525 Ratio
15
F650/F525 10
5
0 1 2 3 4
Time (sec)
25 µm

12. Testing Provided by:
BM Hagen, WJ Lederer, JPY Kao
Center for Biomedical Engineering & Technology
University of Maryland, Baltimore
Medical Biotechnology Center
University of Maryland Biotechnology Institute

13. www.Teflabs.com