Cloning Vector

A SEMINAR ON GENE CLONING VECTORS PRESENTED BY NASREEN SULTAN M.Sc BIOTECHNOLOGY 3 RD SEMESTER

INTRODUCTION ,[object Object],[object Object]

Characteristics ,[object Object],[object Object],[object Object],[object Object]

Types ,[object Object],[object Object],[object Object],[object Object]

Plasmid Vector: pBR322 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Structure of E.Coli plasmid cloning vector pBR322 4362 bp

pUC Plasmids ,[object Object],[object Object],[object Object],[object Object]

Multiple Cloning Site (MCS or Polylinker) .

Screening procedure of cloning vector ,[object Object],[object Object]

Blue/White Selection Only colonies from bacteria that have plasmid IPTG + X-Gal Overnight growth Bacteria with plasmid plus insert Colonies with insert - white Colonies w/o insert - blue

Based on the enzymatic reaction of β -galactosidase

BACTERIOPHAGE VECTORS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

. DNA cloning using phages as vectors

Cosmid vector ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Cloning by using Cosmid vectors

Latest Generation VECTOR: Phagemid- p Bluescript

APPLICATION ,[object Object],[object Object],[object Object],[object Object],[object Object]

MODE OF REPLICATION IN PHAGE M13 VECTOR

Cloning Vector

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Cloning Vector

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