Since plants are used as therapeutic agents, the study was conducted to evaluate the second metabolites of Momordica charantia Linn’s extract and it’s antibacterial activities.

1. Proponents
John Rovic Ngo,Trisha Reyes,Lester Almarez
August2013
Momordica charantia L. Extract as a
Prophylactic Medicine for Gram-
Negative
Bacteria Escherichia coli
A Science Research Project Presented to
The Faculty of Science and Technology

2. Abstract
• Since plants are used as therapeutic agents, the
study was conducted to
evaluate the second metabolites of Momordica
charantia Linn’s extract and it’s
antibacterial activities. Studies on the plant’s
extract itself, 75% ethyl alcohol and
sample free-disk as the control setups using
Disc Agar Diffusion Method.

3. • The microorganism.used is Gram Negative
bacteria Escherichia coli. The maximum activity
was observed against the said bacteria. The
minimum zone of inhibitory concentration ranged
at 10mm on microorganism. As conducted,
Momordica charantia L. was observed to have
antibacterial activity and can be used as
medicinal purposes.

4. Introduction
• Bacteria are living things that are ubiquitous. Most of
them won’t hurt you. Many are helpful for digestion,
destroy disease-causing cells and give the body needed
vitamins. But infectious bacteria can cause severe
illness. They produce themselves quickly inside the
body. Many emit chemicals such as toxins, which can
damage tissues.One of the common bacteria that infect
us is the gram-negative Escherichia coli.
• So, we, the proponents tried to find a resourceful way of
medication for Antimicrobial diseases using Momordica
charantia Linn extract.

5. Some Terms Used
• Momordica Charantia
• Escherichia coli
• Evaporation
• Inoculation

6. Methodology
• Sterilization Of Materials
• Extraction of Plant Material
• Test Organism
• Control Set-Up(s)
• Inoculum Preparation
• Inoculation
• Application of Paper Discs With Extracts
• Gathering Of Results

7. Gathering of Materials

8. Sterilization of Materials

9. Extraction Of Plant Material

10. Test Organisms

11. Research Paradigm

12. Results

13. Legends
Disc: 10 mm diameter
Reactivity Rating: 0 – None (No detectable zone around
or under specimen)
1 – Slight (Some malformed or
degenerated cells under the specimen)
2 – Mild (Zone limited under the
specimen)
3 – Moderate (Zone extends 5 to 10
mm beyond specimen)
4 – Severe (Zone extends greater than
10 mm beyond specimen)
Inhibitory Activity Rating: (+++) complete, (++) partial, (+)
slight and (-) negative

14. Results and Interpretation
• The sample, “Ampalaya Extract”, produced
complete inhibitory activity (+++) with mild
reactivity (2) against test organism, Escherichia
coli; The sample “Alcohol”, which served as
control, had negative inhibitory activity (-) and no
reactivity (0) against the test organism, E.
coli. The sample free disc, which served as
negative control, produced negative inhibitory
activity (-) and no reactivity (0) against the test
organism.

15. Discussion
• In this experiment, 3 set-ups were used to
determine on which will be the most effective
prophylactic medicine for gram –ve bacteria
Escherichia coli diseases.
• From the tabulation, the “Ampalaya Extract”
produced the positive result of reactivity.This
shows the capabilities of Momordica charantia
L.’s extract can be a new medicine for gram –ve
bacteria Escherichia coli.

16. Conclusion
• The proponents’ research proved that the
application of Momordica charantia L.As
significance to be the prophylactic medicine for
E. coli.
• This concludes to the fact that the pure extract of
the said plant is a good Medicine for diseases of
the said bacteria.
• Therefore, the use of the extract of ampalaya
can be an alternative agent for E.coli.

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