Presentation from 10/15/13 on innovative startup producing cost effective nano particle kits to get large amounts of DNA into cells without damaging them. Genetix Fusion LLC founder Mohit Patel explains how nanotech makes this possible and the enormous time and cost problems they're able to address. Join OpenlyDisruptive.org for live access to more disruptive innovation events.

1. The next generation of
Gene Delivery
Mohit B. Patel

2. What is Nano?
Macro
Nano
Millionth
(10-­‐6)
Human
Being
6
feet
tall
Micro
Billionth
(10-­‐9)
Red
Blood
Cell
8
micrometer
DNA
2nm
wide

3. Nanotechnology
Energy
Nanomedicine
Nanosensors
Broad
range
of
applica<ons

4. Genetic Material
Tissue
Gene
Cell
Body
Chromosome
DNA

5. Genetic Disorders
Illness
caused
by
an
abnormality
in
an
individuals
DNA/gene.

6. Gene Delivery
Gene
Delivery
Gene
Expression
Research applications: Genetic disorders, gene therapy, protein
production, functional genomics, disease diagnostics and
agriculture crop enhancement

7. Gene Delivery
Transfection: Laboratory procedure to delivery foreign DNA
into host cells.

8. Physical methods
Electropora5on
Gene
gun
Microinjec5on
Advantage:
Can
transfer
large
DNA
molecules
Disadvantage:
Highly
cytotoxic
and
expensive

9. Biological methods
Advantage:
Stable
transfec<on
Disadvantage:
High
risk
and
<me
consuming

10. Chemical methods
Calcium
phosphate
Lipids
Nanotubes
Advantage:
Good
efficiency
Disadvantage:
Cytotoxic
and
limited
gene<c
informa<on

11. Limitations of Existing Products
• Toxic to cells
$6000 and
30 days / year for
each scientist
• Limited size
(kilobases, kb)
• Poor efficiency
• Time consuming
• Inconsistent results
• Costly
11
“Transfection market
clamors for silver
bullet.”

12. Solution: Janus Transfection Kit™
Janus Transfection Kit™ utilizes a new class of chemical
reagent for DNA transformation and transfection.
New Class = non-lipid, non-viral and
non-dendrimer based transfection reagent
Janus Transfection Kit™
Proprietary
Platform Technology
12

13. Janus Transfection
Reagent™
STEP 1
STEP 2
STEP 3

14. Efficiency: Janus Transfection Kit™
60
50
40
30
20
10
0
Yeast
Colony forming units
Colony forming units
Bacteria
Janus Transfection Promega's Product
Reagent™
600
500
400
300
200
100
0
Mammalian cells
Number of cells
transfomed
0.00%
Janus Transfection
Reagent™
Control
Standard Reagent
Plants
100.00%
50.00%
Janus Transfection
Reagent™
60
50
40
30
20
10
0
Janus Transfection
Reagent™
Control

15. Competitive Advantages
Janus Transfection Kit™ will save each researcher
an estimated 65% of cost and 50% of time.
Company
In-House
Invitrogen
Sigma Aldrich
Promega
Qiagen
DNA Size
<7kb
~9-12kb
~11kb
~7-10kb
~9-12kb
Toxicity
High
High
Low
Low
Medium
Genetix
Fusion
>20kb
None Very High
15
Cell Versatility
Low
High
Medium
Medium
Medium
Price
$ 413
$ 485
$ 333
$ 440
$ 288
$ 350

16. Solution: Janus Transfection Kit™
Enables use of larger DNA
High efficiency
Non-mutagenic and non-cytotoxic
Broad range of applications

17. Transfection Market
$13.4 Billion market
DNA and RNA delivery
$346 Million
Genetix Fusion accessible market
Academia 68% ($235 M)
Pre-packaged kits 75% ($259 M)

18. Critical Milestones
Launch first
product
Genetix Fusion
founded
Alpha
prototype
tested
Initial funding
raised
May
2012
PCT
application
Provisional
patent
Beta prototype
1% Market
(~5500 units)
Controlled
market release
January
2018
January
2014
Establish
operations
Today
January
2015
Strategic
distribution
partner
4% Market
(~28000 units)

19. Now Seeking
1. Academic researchers for beta
testing
2. Collaborative opportunities
3. Distribution partner
4. Seed stage investment

20. REDEFINING TRANSFECTION
Contact us:
mohitpatel87@gmail.com
www.genetixfusion.com
(314) 516-4607