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Identification of AX7503 labeling site on Plk3.
Yongsheng Liu, Ning Jiang et al. J. Biol. Chem. 2007;282:2505-2511
©2007 by American Society for Biochemistry and Molecular Biology

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AX7503 labeling site on Plk3

  • 1. Identification of AX7503 labeling site on Plk3. Yongsheng Liu, Ning Jiang et al. J. Biol. Chem. 2007;282:2505-2511 ©2007 by American Society for Biochemistry and Molecular Biology

Notas do Editor

  1. Identification of AX7503 labeling site on Plk3. A, MS/MS spectrum of the triply charged labeled active site peptide (m/z 1173.7), CYEATDTETGSAYAVK*VIPQSR, of Plk3. The y fragment ions (from y3 to y20) and b fragment ions (from b3 to b11) of the peptides were observed. Fragments y6 (m/z 700), y7 (m/z 951, doubly charged), and y8 (m/z 1000, doubly charged) indicate that the labeling site of the peptide is Lys-16 in the peptide. B, sequence of Plk3 is presented where the peptide identified by LC-MS/MS analysis is indicated in red.