4. Ischemia
Ischemia is a restriction in blood supply
to tissues, causing a shortage of oxygen
and glucose needed for cellular
metabolism (to keep tissue alive).
Ischemia is generally caused by problems
with blood vessels, with resultant damage
to or dysfunction of tissue.
5. Ischemia
Ischemia comprises not only insufficiency
of oxygen, but also reduced availability
of nutrients and inadequate removal
of metabolites.
6. Causes of Ischemia
It can be caused by:
Embolism, thrombosis of
an atherosclerosis artery, or trauma.
Venous problems like venous outflow
obstruction and low-flow states can
cause acute arterial ischemia.
An aneurysm is one of the most frequent
causes of acute arterial ischemia.
7. Causes of Ischemia
Other causes are heart conditions
including myocardial infarction, mitral
valve disease, chronic atrial
fibrillation, cardiomyopathies,
and prosthesis, in all of which thrombi are
prone to develop.
8. Signs and symptoms
Since oxygen is carried to tissues in
the blood, insufficient blood supply causes
tissue to become starved of oxygen.
In the highly aerobic tissues of
the heart and brain, irreversible damage to
tissues can occur in as little as 3–4
minutes at body temperature.
9. Signs and symptoms
The kidneys are also quickly damaged by
loss of blood flow. Tissues with slower
metabolic rates may undergo irreversible
damage after 20 minutes.
Without immediate intervention, ischemia
may progress quickly to
tissue necrosis and gangrene within a few
hours.
10. Signs and symptoms
Paralysis is a very late sign of acute
arterial ischemia and signals the death
of nerves supplying the extremity. Foot
drop may occur as a result of nerve
damage.
12. Cardiac Ischemia
o Cardiac ischemia may be asymptomatic or
may cause chest pain, known as angina
pectoris.
o It occurs when the heart muscle,
or myocardium, receives insufficient blood
flow. This most frequently results
from atherosclerosis.
13. Cardiac Ischemia
o Ischemic heart disease is the most
common cause of death in most Western
countries and a major cause of hospital
admissions.
14. Bowel Ischemia
o Both large and small intestine can be
affected by ischemia.
o Ischemia of the large intestine may result
in an inflammatory process known
as ischemic colitis.
o Ischemia of the small bowel is
called mesenteric ischemia.
15. Brain Ischemia
o Brain ischemia is insufficient blood flow to
the brain, and can be acute or chronic.
o Acute ischemic stroke is a neurologic
emergency that may be reversible if
treated rapidly.
o Chronic ischemia of the brain may result
in a form of dementia called vascular
dementia. ".
16. Brain Ischemia
o A brief episode of ischemia affecting the
brain is called a transient ischemic
attack (TIA), often referred to as a "mini-
stroke”.
18. Cutaneous Ischemia
o Reduced blood flow to the skin layers may
result in mottling or uneven, patchy
discoloration of the skin.
19. Pathophysiology of Ischemia
Treatment of Ischemia
Introduction of Necrosis
Causes of Necrosis
Changes in necrotic cell
Fate of necrotic cell
Presented by:
Hamayoun Ashraf
Roll No. 06
20. Pathophysiology
Ischemia results in tissue damage in a
process known as ischemic cascade. The
damage is the result of the build-up
of metabolic waste products, inability to
maintain cell membranes, mitochondrial
damage, and eventual leakage
of autolyzing proteolytic enzymes into the
cell and surrounding tissues.
21. Pathophysiology
Restoration of blood supply to ischemic
tissues can cause additional damage
known as reperfusion injury that can be
more damaging than the initial ischemia.
Reintroduction of blood flow brings
oxygen back to the tissues, causing a
greater production of free
radicals and reactive oxygen species that
damage cells
22. It also brings more calcium ions to the
tissues causing further calcium
overloading and can result in potentially
fatal cardiac arrhythmias and also
accelerates cellular self-destruction.
The restored blood flow also exaggerates
the inflammation response of damaged
tissues, causing white blood cells to
destroy damaged cells that may otherwise
still be viable.
23. Treatment
Early treatment is essential to keep the
affected limb viable.
The treatment options include injection of
an anticoagulant, thrombolysis,
embolectomy, surgical revascularisation,
or amputation.
24. Treatment
Anticoagulant therapy is initiated to
prevent further enlargement of
the thrombus. Continuous
IV unfractionated heparin has been the
traditional agent of choice.
25. Necrosis
Necrosis is the type of cell death that is
associated with loss of membrane integrity
and leakage of cellular contents culminating
in dissolution of cells, largely resulting from
the degradative action of enzymes on
lethally injured cells.
26. Causes of Necrosis
• Anoxia
• Ischemia
• Physical agents
• Chemical agents
• Biological agents
• Hypersenstivity
27. Changes in necrotic cell
Necrosis is characterized by changes in the
cytoplasm and nuclei of the injured cells.
Cytoplasmic changes: Necrotic cells show
increased eosinophilia. The cell may have a
more glassy, homogeneous appearance,
mostly because of the loss of glycogen
particles. Myelin figures are more prominent
in necrotic cells than during reversible injury.
28. Changes in necrotic cell
Nuclear changes. Nuclear changes
assume one of three patterns, all due to
breakdown of DNA and chromatin.
• The basophilia of the chromatin may fade
(karyolysis), presumably secondary to
deoxyribonuclease (DNase) activity.
29. Changes in necrotic cell
A second pattern is
• pyknosis, characterized by nuclear
shrinkage and increased basophilia; the
DNA condenses into a solid shrunken
mass.
In the third pattern,
• karyorrhexis, the pyknotic nucleus
undergoes fragmentation
30.
31.
32. Fates of necrotic cells
Necrotic cells may persist for some time or
may be digested by enzymes and disappear.
Dead cells may be replaced by myelin
figures, which are either phagocytosed by
other cells or further degraded into fatty
acids. These fatty acids bind calcium salts,
which may result in the dead cells ultimately
becoming calcified.
35. Coagulative necrosis
“In this type of necrosis, the necrotic cell retains its
cellular outline for several days”
• Coagulative necrosis typically occurs in solid
organs such as kidney, heart and adrenal gland
usually as a result of deficient blood supply and
anoxia.
Examples
• Myocardial infarction
36. Mechanism
• Denaturation of protein is the basic mechanism
of coagulative necrosis
• The injury and the subsequent increasing
acidosis
denatures not only the structural proteins but
also the enzymic proteins, thus blocking the
cellular proteolysis.
37. Morphology:
• Preservation of basic structural outline of
the coagulated cells
• Appears as a mass of coagulated, pink
staining homogenous cytoplasm
40. Liquefactive necrosis
It is the type of necrosis that occurs due to
autolytic and heterolytic actions of enzymes that
convert the proteins of cells into liquid.
It is characterized by softening and liquifaction of
tissue.
Examples
• Ischemic necrosis of brain.
• Suppurative inflammation.
41. Mechanism:
• Enzymatic degradation of proteins is the
basic mechanism of liquefactive necrosis
Morphology:
o Complete loss of cellular detail
o Cellular outline is also destroyed
44. CASEOUS NECROSIS
• Combination of coagulative and
liquefactive necrosis
• Characterized by the presence of soft, dry,
cheesy homogenous necrotic material.
• It is not liquefied.
45. Examples
• Principaly in the center of tuberculous
granuloma.
Morphology:
• Microscopically the necrotic focus is composed
of structureless amorphous granular debris
enclosed within a ring of granulomatous
inflammation.
47. Necrosis in special sites
Fat Necrosis
It occurs in two forms:
• Enzymatic fat necrosis
• Traumatic fat necrosis
48. Enzymetic Fat Necrosis
Most commenly seen in acute pancreatitis.
“Refers to the necrosis in adipose tissue, induced
by the action of pancreatic enzymes which are
lead due to trauma to the pancreas”
Morphology :
• Chalky white opaque spots surrounded by
inflammatory margins are seen
• Necrotic area shows acute inflammatory
changes with dissolved fat cells
49. Traumatic Fat necrosis
It occur following severe injury to the tissues with
high fat content such as the breast
subcutaneous tissue and abdomen.
Morphology
• Foam cells and gaint cells are seen.
necrotic foci contain a lot of phagocytes
containing fat known as foam cells
50.
51. Fibrinoid necrosis
• Type of connective tissue necrosis especially
affecting arterial walls.
Mostly seen in two conditions
• Auto immune diseases e.g
Rheumaic fever
SLE
• Malignant hypertension.
53. Gangrenous necrosis
• Gangrene is the necrosis of tissue with
superadded putrefaction (enzymatic
decomposition).
• It is the clinical condition in which extensive
tissue necrosis is complicated to a variable
degree by secondary bacterial infection.
• Gangree= Necrosis + infection + putrefaction
54.
55. Causes of Gangrene
Arterial obstructon due to:
• Thrombosis of atherosclerotic artery
• Embolus
• Diabetes:- atherosclerotic artery , loss of sensation
results reapeted trauma & increase chances of
infection
Infection
• Gas gangrene
• Gangrene of scrotum
58. Dry Gangrene
It is usually secondary to slow occlusive vascular
disease
Etiology
Gradual loss of arterial supply to an organ or tissue as
happens in
Arteriosclerosis
Atherosclerosis
Trauma
Ergot poisoning
59. Common sites
limbs; especially foot
Pathogenesis:
• It is a traditional term used to describe the
infarction of the limbs.
• It is not true gangrene because the infection in
necrotic tissue is insignificant and putrefaction is
absent or minimal.
• The necrotic area becomes black due to
breakdown of hemoglobin and formation of iron
sulfide
61. Wet Gangrene
• It is a type of gangrene in which tissue appears moist.
• It results from severe bacterial infection superimposed
on necrosis
Common sites
Intestine
Appendix
Limbs
62. Pathogenesis
• It is a true gangrene because it shows the
severe infection and putrefaction of tissue with
edema and foul smell.
• Arterial obstruction present.
• blackening of the tissue is due to formation of
iron sulphide
It is not clearly demarcated from adjacent
healthy tissues.
63.
64. Gas Gangrene
“In this type of gangrene bacterial infection causes necrosis
and then gangrene with abundant gas formation in the tissue”
• Gas gangrene=wet gangrene + gas formation
Predisposing factors:
• Foreign bodies in wound cause tissue ischemia
• Foreign bodies favour infection
• Contamination of wound by soil is dangerous bqz its ionisable
calcium salts and silicic acid may lead to tissue necrosis.
• Infection by aerobic organisms at the same time serve to
produce anaerobic environment that is favourable for
anaerobic clostridia.
65. Etiology:
Two groups of clostridia cause gas gangrene
• Saccharolytic:
Clostridia profringes
• Proteolytic:
Clostridia isolyticum
66. Pathogenesis:
• Deep wound----anerobic condition---caused by
spores of clostridia
• Necrosis of muscle fiber occur
• Fermentation of muscle carbohydrate occur with
formation of lactic acid and gas.
• Arterial supply of the area is cut down
• Muscles become greenish- black due to iron
sulphide & foul smell
67. Common Sites:
• Muscles
• Liver
Complicatons:
• Rapidly spreading gangrene
• Shock and hemolytic anemia
Treatment of gangrene:
• Treatment of predisposing factor:
• Amputation:
Surgical removal of gangrene tissue to prevent spreading of
the infection to the healthy tissue.
70. Hypoxia
An abnormally reduced O2 supply to
tissue
A pathological condition in which the body
as a whole (generalized hypoxia) or a
region of the body (regional hypoxia) is
deprived of adequate oxygen supply.
71. Causes of Hypoxia
Inadequate oxygenation
Deficiency of oxygen in atmosphere
Hypoventilation (neuromuscular disorders)
Pulmonary disease
Hypoventilation due to increased airway
Resistance.
72. Causes of Hypoxia
Diminished respiratory membrane
diffusion
Inadequate oxygen transport to tissues
Anaemia or abnormal Hb
General circulatory deficiency
Localized circulatory deficiency
Tissue oedema
73. Causes of Hypoxia
Inadequate tissue capability of using
oxygen
Poisoning of cellular oxidation enzymes
Diminished cellular metabolic capacity for
using oxygen, because of toxicity, vitamin
deficiency or other factors
74. Effects of Hypoxia on body
Hypoxia, if severe
• can cause death of cells throughout the
body
In less severe degrees
• Depressed mental activity, sometimes
results in coma
• Reduced work capacity of muscles
75. Types of Hypoxia
Atmospheric Hypoxia (Hypoxic Hypoxia)
Hypoventilation Hypoxia
Anemic Hypoxia
Stagnant or ischemic Hypoxia
Histotoxic or cytotoxic Hypoxia
76. Atmospheric Hypoxia (Hypoxic Hypoxia)
An insufficient O2 supply reaches the
blood due to
Decreased atmospheric PO2 at high
altitudes
Reduced alveolar ventilation
Impaired alveolar gas exchange
77. Hypoventilation Hypoxia
A reduced amount of air enters the alveoli
in your lungs, resulting in hypoxia and
hypercapnia
COPD
Scoliosis, nasal septum deformation
Weakened respiratory muscles - motor
neurone disease
79. Stagnant or ischemic Hypoxia
Insufficient O2 reaches the tissue due to
reduced blood flow
Systemic or local
80. Histotoxic or cytotoxic Hypoxia
Impaired utilization of O2 by the tissues
despite a sufficient supply of O2 in the
mitochondria
Cyanide poisoning
Cyanide (HCN) blocks oxidative cellular
metabolism by inhibiting cytochromoxidase