1. Curriculum Vitae
Meghan Ashley Holder
1141 Madeline Heights
Breaux Bridge, LA 70517 US
Mobile: 985-518-8783; Email: meghanaholder@gmail.com
Work Experience:
Tanner Services/Environmental Division, LLC: 104 Abigayle's Row, Scott, LA 70583
Dates of employment: 12/2013 - 04/2016 - Salary: $60,000.00 USD/ year
Hours per week: 40 - Title: Environmental Engineer
Duties, Accomplishments and Related Skills: Chemical lab maintenance, in house experiments
with products and field materials; inspections of company facilities to ensure compliance with
state requirements on drinking water and NPDES standards; provide improvements and solutions
to current and possible problems with such facilities; field sampling in Texas and Louisiana thus
far which included traveling to sites to sample liquids and solids for oil and gas industry on
active, non-active, and possible superfund sites thought to be contaminated with heavy metals,
PCBs, PAHs, BTEX, TPH, Oil and Grease, NORM and other RCRA classified hazardous
wastes; author SOPs for field sampling, analytical test requirements respective to state codes, lab
techniques, and managing shipping logistics of samples and testing requirements with nationally
accredited analytical labs; written and electronic logs of locations and sample quality/quantity/
site description; generation of SOPs for recycling protocols used intra-company use as well as
via customer; prepare and present company power point presentations for advertising, pilot study
results, and site conditions; provide technical assistance in the modification and blending of
company products for optimal results, along with their application on site; supervising and
training employees in proper safety and application techniques; oversee de-watering, back filling
and closure projects; ensured company and customer compliance with state and federal
regulations in hazardous waste/exemption and disposal/recycle/remediation(Texas Railroad
Commission, LA-DNR, RCRA 40 CFR); generate, evaluate and approve reports for company
and customer(s) that would include explanations of analytical lab reports, sampling logs, state
and federal guideline compliance/non-compliance, and closure/remediation techniques; office
manager duties of filing, conversion of MSDS to SDS due to GHS requirement changes, writing
invoices/POs, chemical orders, supply orders, payments, handling per diem for employees,
generating job control costs/ earning reports, and payroll.
The Pool Guy, LLC: 204 Burgess Dr., Broussard, LA 70518
Dates of Employment: 10/2013 - 12/2013 ∙ Salary: $11.00 USD/ hour
Hours per week: 50(+) ∙ Title: Pool Tech
Duties, Accomplishments and Related Skills: Customer pool care, operations maintenance,
and minor repairs of pool pumps and filters; water chemistry testing with corrections; customer
supply and repair orders; and shop inventory.
Supervisor: James Hutchinson (337-303-9003)
Okay to contact this Supervisor: Yes
Page 1 of 7

2. Work Experience: (continued)
USGS (Five Rivers/AKIMA): 700 Cajundome Blvd., Lafayette, LA 70506
Dates of Employment: 06/2011 - 08/2013 ∙ Salary: $16.00 USD/ hour
Hours per week: 40 ∙ Title: Lab Analyst (contractor)
Duties, Accomplishments and Related Skills: Responsible for wet and dry lab maintenance;
logistics of shipping/receiving time sensitive fish tissue/organ samples; generating needed
buffers; lab safety and MSDS (at that time) logs; fish blood osmolality measurements;
processing of fish blood, eyes, testes, and sperm for microscopy and cytometery analyses;
interpretation and analysis of quantitative and qualitative microscopy; cytometry; animal care
(fishes, frogs, crawfish); hematological cell extraction and separation (via centrifuge) to stain for
microscopy; assistance in sample logging, processing, and analyzing data for a Natural Resource
Damage Assessment (NRDA) project; assistance in completing a state license for housing
Tilapia; sexing of Tilapia (Oreochromus nilotecus); assist in preparing documents needed for an
Institutional Animal Care and Use Committee (IACUC) permit; assist in preparing permit to
house endangered pallid sturgeon (Scaphirynchus albus); assist in extracting and process fluid
from eyes of both farmed and feral Asian carp for genome determination as diploid or triploid to
decipher population movements; analyze catostomid sperm for abnormalities and counts as an
indicator species of the Columbia river in the northwest USA; preparing stains for cell
identification; optimize staining procedures for pallid sturgeon blood; anaesthetizing and
extracting blood from several spp. of fish; culling and logging of lab animals used in
experiments; write and review documents for literature publications of results; arrange analytical
data for statistical analysis and graphic display; attend and present findings at regional and
national meetings for American Fisheries Society; aquatic system cleaning, maintenance,
structure repairs, design and installation of new air delivery system.
Supervisor: Dr. Jill A Jenkins (225-324-0416)
Okay to contact this Supervisor: Yes
University of Louisana, Lafayette / USGS: 700 Cajundome Blvd., Lafayette, LA 70506
Dates of Employment: 11/2010 - 06/2011 ∙ Salary: $13.00 USD/ hours
Hours per week: 20 to 30 ∙ Title: Lab Tech (intern)
Duties, Accomplishments and Related Skills: Chemical lab and wet lab maintenance and
animal care; qualitative and quantitative microscopy of fish sperm; generation of needed buffers;
osmolality of buffers and biological samples; generation of data spreadsheets/ logs; bleeding and
sperm expression of fish; culling and logging of lab animals used in experiments; lab safety
including MSDS organization and updates.
Supervisor: Dr. Jill A Jenkins (225-324-0416)
Okay to contact this Supervisor: Yes
Page 2 of 7

3. Work Experience: (continued)
University of Louisana, Lafayette / NSF: E. University Cir., VLW410, Lafayette, LA 70503
Dates of Employment: 01/2010 - 12/2010 ∙ Salary: $11.00 USD/ hour
Hours per week: 30 ∙ Title: Research Education for Undergraduates (intern)
Duties, Accomplishments and Related Skills: National Science Foundation-Research
Education for Undergraduates (REU) intern; analyzed fish bio-diversity data from two
expeditions to southeastern Peru; amazonian fish identification, staining, photography, and
preservation; prepared and presented poster publication at a university and regional level
concerning the fish fuana diversity and richness within an area; aided in graphics for publications
within and outside the university; lab animal care; aided in the design and construction of web
pages for "Proyecto Alto Purus." (http://www.ucs.louisiana.edu/~jxa4003/Alto%20Purus.html).
Supervisor: Dr. James S. Albert (337-482-6627)
Okay to contact this Supervisor: Yes
South Lousiana Community College: 1101 Bertrand Dr., Lafayette, LA 70506
Dates of Employment: 07/2009 - 12/2010 ∙ Salary: $11.00 USD/ hour
Hours per week: 30 ∙ Title: Math and Science Tutor
Duties, Accomplishments and Related Skills: Tutor students in multiple aspects of math and
science including: basic math, algebra, physics, calculus, chemistry, anatomy, and biology.
Supervisor:Heather Maira
Okay to contact this supervisor: No
University of Louisana, Lafayette / USGS: 700 Cajundome Blvd., Lafayette, LA 70506
Dates of Employment: 09/2009 - 11/2010 ∙ Salary: $11.00 USD/ hour
Hours per week: 30 ∙ Title: Field sampler (intern)
Duties, Accomplishments and Related Skills: Tag and recapture of green tree frogs (Hyla
cinerea); input of alphanumeric tag information and animal stat data in a electronic program for
statistical analysis, was part of an an ongoing population dynamics and movement study.
Supervisor: Dr. Jacoby Carter (337-781-4882)
Okay to contact this Supervisor: Yes
Cricket's Pool World: 1100 Rue Du Belier, Lafayette, LA 70506
Dates of Employment: 06/2009 - 09/2009 ∙ Salary: $11.00 USD/ hour
Hours per week: 40 ∙ Title: Pool Tech
Duties, Accomplishments and Related Skills: In store customer service; water chemistry
testing and generate recommendations for corrections. Handling of funds and end of day
deposits.
Supervisor: Katherine or Scott Benoit (337-984-7626)
Okay to contact this Supervisor: Yes
Page 3 of 7

4. Work Experience: (continued)
City of Clearwater, Florida: 900 N. Martin Luther King Blvd., Clearwater, FL 33755
Dates of Employment: 10/2007 - 06/2009 ∙ Salary: $24,000.00 USD/year
Hours per week: 40 ∙ Title: Aquatics Progmmer
Duties, Accomplishments and Related Skills: Customer service at the North Greenwood
Recreational Facilty; water chemistry and correction/ammendments needed for public use of
swimming pools; attend National Recreation and Parks Association conferences and trainings for
Aquatic Progammers; teach and maintain certifications for safety, NIMS (FEMA) training,
lifeguarding, AED, CPR, first aid, and First Repsonder courses; supervise a staff of 12 lifeguards
during 3/4 of the year; manage camp attendance dynamics; manage major repairs/improvements;
perform minor repairs/maintenance which included regulary backwashing, chemical orders,
safety product orders; correspondence with other City of Clearwater aquatic/recreation sites;
generate end of year profit reports and attendance logs; create and supervise festivity events;
comptroll city wide events; manage/supervise swim team practices and events; coordinate and
teach swim lessons (multi-level); received employee of the month within first six months of
employment (among 10 employees), received employee of the year within first year of
employment (among 300 employees); fill in at multiple city recreation/pool/nature park sites.
Supervisor: Barbara Moon/ Kathy LaTerza/ Mark Roberson (727-462-6276)
Okay to contact this Supervisor: Yes
Education:
University of Louisiana, Lafayette: Lafayette, LA
Baccalaureate’s Degree 12/2010
GPA: 3.14 of a maximum 4.0
Credits Earned: 46 Semester hours
Major: Resource (Marine) Biology/ biodiversity Minor: Chemistry
Relevant Coursework, Licenses and Certifications: biological diversity, microbiology,
ichthyology, organic chemistry I and II with labs, environmental toxicology, physiology, physics
II (electricity/currents)
Honors: Dean's List
University of South Florida, Tampa: Tampa, FL
Coursework Completed towards Baccalaureate’s degree
Credits Earned: 21 Semester hours
Major: Marine Biology
Relevant Coursework, Licenses and Certifications: global conservation, wetland
environments, physics I with lab, marine biology, principles of ecology with lab
Page 4 of 7

5. Education: (continued)
St. Petersburg Community College: Saint Petersburg, FL
Associate's Degree 06/2007
Credits Earned: 50 Semester hours
Major: Visual Arts
Relevant Coursework, Licenses and Certifications: Fundamentals of Biology, qualitative and
analytical chemistry II with lab; ceramics I and II, drawing I and II, design I
Eckerd College: Gulfport, FL
Coursework Completed towards Baccalaureate’s degree
Credits Earned: 66.5 Semester hours
Major: Marine Biology Minor: Chemistry
Relevant Coursework, Licenses and Certifications: introduction to marine science, marine
geology, genetics, cell biology, oceanography, mathematical modeling, Calculus I and II,
independent research of cownose ray migration (Rhinoptera bonasus), marine invertebrate
biology, general Chemistry I with lab
Job Related Training:
∙ Fork lift operation
∙ PEC safety basic (includes SafeLand and SafeGulf)
∙ PADI open water diver certification
CEU credits for the following attendences:
"Remediaton with Nano-Zero Valent Iron (nZVI): Recent Developments,
Applications, and Global Perspectives." at the Ninth International Conference on
Remediation of Chlorinated and Recalcitrant Compounds." May 18-22, 2014;
Monterey, CA.
"Soil and Groundwater Remediation Technology Seminar." November 12, 2015;
Baton Rouge, LA.
Language Skills:
Language Spoken Written Read
English Advanced Advanced Advanced
Spanish-
American
Novice Novice Novice
Page 5 of 7

6. Affiliations:
∙ American Fisheries Society - Previous member
∙ National Parks and Recreation Association - Previous member
∙ Eckerd College Search and Rescue (EC-SAR) - Previous student member
∙ Institution Animal Care and Use Committee (IACUC) - Current community review
member of the Wetlands Research Center in Lafayette, LA
Professional Publications:
"Improved Peripheral Blood Extraction and Staining Protocols Modified for Pallid Sturgeon
(Scaphirynchus albus)." (in review) Applied Ichthyology; National Wetlands Research
Center, Lafayette, LA. Authors: Meghan A Holder, Jill A. Jenkins, Tiffany S. Smoak,
Rassa Draugilas O-Dale.
"An Improved Protocol for Hematology Staining with Pallid Sturgeon." (Poster) 2013- Presented
at the National American Fisheries Society meeting, September 8-12, 2013; Little Rock,
AR. Authors: Meghan A. Holder (1), Tiffany S. Smoak (2), Jill A. Jenkins (3).
"Comparisons of Biomonitoring Results from Davis Pond and Caernarvon Mississippi River
Freshwater Diversions." (Poster) 2012- Presented at the Southeastern Division American
Fisheries Society meeting, January 2-29, 2012; Biloxi, MS. Authors: Heahter Olivier (1),
Beth B. Bourgeois (2), Paul J. Conzelmann (3), Meghan A. Holder (4), Rassa Draugelis-
Dale (4), Jill A. Jenkins (4).
"Fishes of the Fitzcarrald, Peruvian Amazon." (Illustrations in Book) 2012- Lulu Press; Releigh,
NC. Authors: J.S. Albert, T.P. Carvalho, J.A. Chuctaya, P. Petry, R. E. Reis, B. Rengifo,
and H. Ortego.
"Aquatic Bio-Diversity in the Amazon: Habitat Specialization and Geographic Isolation Promote
Species Richness." (article) 2011 -Animals, 1 (2), 205 -241; doi:10.3390ani1020205.
Authors: James S. Albert, Tiago P. Carvalho, Paulo Petry, Meghan A. Holder, Emmanuel
L. Maxime, Jessica Espino, Isabel Corahua, Roberto Quispe, Blanca Rengifo, Hernan
Ortega, and Roberto E. Reis.
"Aquatic Bio-diversity in the Amazon: Headwater Fish Fauna of the Fitzcarrald Arch." (poster)
2010 - Presented at the Graduate Student Symposium, University of Louisiana, Lafayette
in Lafayette, LA; 2011 - Presented at the Southeastern Evolutionary and Ecology
Conference in Auburn, AL; Authors: Meghan A. Holder, Tiago P. Carvalho, and James
S. Albert.
Page 6 of 7

7. Professional Publications: (continued)
"Tag Retention of the New Model of Alpha-Tags from Northwest Marine Technology in Green
and Squirrel Treefrogs (Hyla cinerea and H. squirella): Stiffer Tags Cause Many More
Problems." (Poster) 2010- Presented at The Ernie Liner Biennial Herpetological Research
Group Meeting, November 19-21, 2010; LUMCON Cocodrie, LA. Authors: Jacoby
Carter, Jeromi Hefner, Meghan Holder, Forest Huval, Kelsie Hayes, Arianne Martin and
Lance Renoux.
References:
Name Employer Title Phone/ E-mail
Fadi Raad Tanner Services Field Superintendent 956-607-6986
fraad@tannerservices.net
James S. Albert (*) University of Louisiana Professor of Biology 337-482-6627
Lafayette jalbert@louisiana.edu
Jeromi Hefner Acadian Ambulance IT specialist 337-247-0544
Jeromi.hefner@gmail.com
Lance Renoux Ocean Associates, Inc. Fisheries Biologist II 337-349-8584
Lance.renoux@gmail.com
Dr. Jill A. Jenkins (*) USGS Senior Research Scientist 225-324-0416
jenkinsj@usgs.gov
Dr. Jacoby Carter (*) USGS Research Ecologist 337-781-4882
carterj@usgs.gov

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