2. Kinetic Data
0% Heavy Water Kinetic Data
#1 #2 #3 #4 Ave. S.D.
[Chor.] Velocity Velocity Velocity Velocity Velocity Velocity
μM μM/h μM/h μM/h μM/h μM/h μM/h
5 1.181371 1.181371 1.121925 1.189988 1.164427 0.03706
10 2.147597 2.249293 1.769266 2.351582 2.233895 0.088926
15 3.044991 3.057483 3.267 3.265958 3.158857 0.124375
20 4.206945 3.861575 3.883357 4.171248 4.03078 0.183602
25 5.136608 5.0539 5.033617 5.033617 5.074707 0.054557
[YbtS] Vmax KM kcat kcat
μM μM/h μM/h h-1
min-1
5.328 25.52309 104.6403 4.790369 0.0798
25% Heavy Water Kinetic Data
#1 #2 #3 Ave. S.D.
[Chor.] Velocity Velocity Velocity Velocity Velocity
MicroM μM/h μM/h μM/h μM/h μM /h
5 1.168186 1.038506 1.023822 1.099487 0.079121
10 1.854019 1.854019 1.661825 1.833531 0.162435
15 2.815416 3.014581 2.720458 2.850151 0.150107
20 3.288733 3.5931 3.280242 3.387357 0.178228
25 4.084343 4.46025 4.084343 4.167683 0.26107
[YbtS] Vmax KM kcat kcat
μM μM/h μM/h h-1
min-1
Vmax Ratio: 2.203512
5.328 11.58291 48.42644 2.17397 0.0362 Vmax/KM Ratio: 1.019762
50% Heavy Water Kinetic Data
#1 #2 #3 Ave. S.D.
[Chor.] Velocity Velocity Velocity Velocity Velocity
μM μM/h μM/h μM/h μM/h μM/h
5 0.946719 0.818639 1.220739 0.991428 0.045911
10 1.653367 1.534677 2.03645 1.759076 0.069959
15 3.141077 2.53053 2.395098 2.332281 0.205916
20 4.024962 2.729533 4.024962 3.149285 0.259462
25 6.331194 3.360068 6.70979 3.600121 0.147808
[YbtS] Vmax KM kcat kcat
μM μM/h μM h-1
min-1
Vmax Ratio: 2.675478
4.632 9.539636 43.38925 1.790472 0.0298 Vmax/KM Ratio: 1.109391
Sample Calculation
[Chorismate] Fluor. Int Fluor. Int Velocity 1/[Chorismate] 1/Velocity

3. μM dil. corr. μM/h μM-1
h/μM
5 121325 174708 1.181371 0.2 0.846474
10 220555 317599.2 2.147597 0.1 0.465637
15 312716 450311 3.044991 0.066667 0.328408
20 432047 622147.7 4.206945 0.05 0.237702
25 527522 759631.7 5.136608 0.04 0.194681
Slope 4.044992
Intercept 0.045138
[YbtS] Vmax KM kcat kcat
μM μM/h μM h-1
min-1
5.328 22.15433 89.61409 4.158095 0.0693
Double Inverse Plot (0%Heavy Water)
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
0 0.05 0.1 0.15 0.2 0.25
1/[Chorismate]
1/velocity
Series1
Linear (Series1)
0%, 25% and 50% Heavy Water Comparison
0
0.2
0.4
0.6
0.8
1
1.2
-0.05 0 0.05 0.1 0.15 0.2 0.25
1/[Chorismate]
1/Velocity
Light Water
25% Heavy Water
50% Heavy Water
Linear (Light Water)
Linear (25% Heavy Water)
Linear (50% Heavy Water)