1. Advancing Life Sciences

2. A national resource
SciLifeLab, Science for Life
Laboratory, is a Swedish national
center for molecular biosciences,
with the mission to develop, use and
provide advanced technologies for
applications in health and environ-
mental research. National funding
makes our services and expertise
available to researchers in all of
Sweden. The infrastructure is mainly
located in Stockholm and Uppsala but
we also offer services at other Swedish
universities.
We offer a cross-disciplinary research
setting that interacts with healthcare,
Sweden’s largest center for life sciences
authorities and industry to meet the
need for new clinical methods and
a better environment. In addition,
SciLifeLab provides education for
students and researchers at all levels.
Our technology platforms
The SciLifeLab national infrastructure
encompasses a multitude of biomo-
lecular technologies and bioinformat-
ics services. On the following pages,
we present all current platforms and
their associated facilities.

3. Affinity Proteomics offer anti-
body-based protein analysis and im-
aging. This includes the investigation
of protein properties such as inter-
actions, modifications, expression
patterns in tissues, abundance in body
fluids and subcellular localization, as
well as binding selectivity.
These analyses are important for
understanding biological processes
and disease pathways, as well as to
identify and validate new drug targets
and diagnostic markers.
We offer advanced fluorescence
microscopy and spectrometry for
visualization and quantification on
the biological micro- and nanoscale.
The Advanced Light Microscopy
(ALM) facility has specialized compe-
tence and instrumentation for cellular
imaging in super-resolution, includ-
ing 3D-SIM, 3D-PALM/STORM, and
3D-STED microscopy.
The Fluorescence Correlation Spec-
troscopy (FCS) facility offers special-
ized competence and fluorescence
fluctuation instrumentation (FCS,
FCCS, RICS, PCH, FRET-FCS) for
live cell studies, suitable for monitor-
ing and imaging molecular dynamics
and interactions down to a single
molecule level.
Facilities
Advanced Light Microscopy
hans.blom@scilifelab.se
Fluorescence Correlation
Spectroscopy
stefan.wennmalm@scilifelab.se
Facilities
Biobank Profiling
jochen.schwenk@scilifelab.se
Cell Profiling
emma.lundberg@scilifelab.se
Fluorescence Tissue Profiling
jan.mulder@scilifelab.se
Mass Cytometry
florence.sjogren@liu.se
petter.brodin@scilifelab.se
PLA Proteomics
masood.kamali@igp.uu.se
Protein and Peptide Arrays
maja.neiman@scilifelab.se
Tissue Profiling
per-henrik.edqvist@igp.uu.se
Affinity Proteomics Bioimaging

4. Chemical Biology Consortium Swe-
den (CBCS) coordinates, and makes
available, powerful infrastructures
for the discovery, development and
utilization of small molecules for
applications in all areas of life science.
CBCS provides expertise within assay
development, screening, chemin-
formatics as well as computational,
medicinal and enabling chemistry,
target identification and compound
pharmaceutical profiling.
CBCS hosts a number of compound
collections consisting of both syn-
thetic and natural compounds for
biochemical, cellular and phenotypi-
cal screening.
Facilities
Laboratories for Chemical Biology
Umeå (LCBU)
mikael.elofsson@chem.umu.se
The Laboratories for Chemical
Biology at Karolinska Institutet (LCBKI)
annika.jensen@scilifelab.se
Uppsala Drug Optimization and Phar-
maceutical Profiling (UDOPP)
pawel.baranczewski@scilifelab.uu.se
Chemical Biology Consortium Sweden

5. We support integrated drug
discovery and development projects
utilizing the facilities within the
platform, which represent a mini-
mal toolbox needed for early drug
discovery. Through these we provide
industry standard infrastructure, ex-
pertise, and strategic support to help
progress projects towards a preclinical
proof-of-concept.
Platform directors
Per Arvidsson, Karolinska Institutet
per.arvidsson@scilifelab.se
Kristian Sandberg, Uppsala University
kristian.sandberg@scilifelab.uu.se
Facilities
ADME (Absorption Distribution,
Metabolism Excretion) of Therapeutics
(UDOPP)
Biochemical and Cellular Screening
Biophysical Screening and Character-
ization
Human Antibody Therapeutics, Stock-
holm and U-READ, Lund
In Vitro and Systems Pharmacology
Medicinal Chemistry – Hit2Lead
Medicinal Chemistry – Lead Identifi-
cation
Protein Expression and Characteriza-
tion
Drug Discovery and Development

6. High throughput methods are trans-
forming basic biological and medical
science. It is now possible to apply
a holistic perspective and examine
all genes and many proteins, their
expression, regulation and function
in numerous organisms, in any tissue,
even at the level of single eukaryotic
or prokaryotic cells.
Services offered at our facilities in-
clude single-cell capture followed by
genomic and transcriptomic analysis
by next-generation sequencing and
protein analysis using proximity-ex-
tension, as well as high-throughput
screening of extensive siRNA and
chemical compound libraries in
various assays.
Facilities
Eukaryotic Single Cell Genomics
sten.linnarsson@ki.se
Karolinska High Throughput Center
(KHTC)
anders.eriksson@ki.se
Microbial Single Cell Genomics
stebe@ebc.uu.se
Single Cell Proteomics
caroline.gallant@igp.uu.se
Functional Genomics
The Swedish Metabolomics Centre in
Umeå (SMC) provides service in the
field of metabolomics using chro-
matographic techniques in combina-
tion with mass spectrometry.
SMC offers all analytical steps from
sample preparation to data process-
ing, for both untargeted and targeted
analysis of small molecules. The ser-
vice platform works as a professional
consultant facility and is divided into
two parts; consulting and service.
Facilities
Swedish Metabolomics Centre (SMC)
thomas.moritz@slu.se
Metabolomics

7. Bioinformatics provides custom-
tailored support beyond the pre-
defined analyses typically offered
by the data-generating platforms at
SciLifeLab. We offer bioinformatics
short-term and long-term support as
well as genome annotation service for
novel genomes and assistance with
data publishing.
The platform also provides computing
and storage resources for large-scale
biological data including know-how,
design and implementation of these
resources for biological data. Further-
more, we can provide feedback and
assistance in experimental planning
and design.
Facilities
Bioinformatics Compute and Storage
(UPPNEX)
ola.spjuth@scilifelab.se
Bioinformatics Long-term Support
(WABI)
bjorn.nystedt@scilifelab.se
thomas.svensson@scilifelab.se
Bioinformatics Short-term Support
and Infrastructure (BILS)
bengt.persson@scilifelab.se
mikael.borg@scilifelab.se
Systems Biology
nielsenj@chalmers.se
thomas.svensson@scilifelab.se
National Bioinformatics Infrastructure Sweden

8. The National Genomics Infra-
structure (NGI) provides access to
technology for massively parallel/
next-generation DNA sequencing and
genotyping. Modern genome analyses
critically depend on expertise in com-
putational biology, which is closely
integrated with NGI to optimize
throughput, data handling, and basic
analysis.
Facilities
NGI Stockholm
(Genomics Applications)
max.kaller@scilifelab.se
NGI Stockholm
(Genomics Production)
genomics_orders@scilifelab.se
NGI Uppsala
(SNP&SEQ Technology Platform)
contact@genotyping.se
seq@medsci.uu.se
NGI Uppsala
(Uppsala Genome Center)
inger.Jonasson@scilifelab.uu.se
National Genomics Infrastructure

9. The aim for the Next-Generation
Diagnostics platform is to meet
clinical diagnostic needs in health
care by providing service and support
to promote and adapt the use of
high-throughput techniques in rou-
tine diagnostics.
These technologies include next-gen-
eration sequencing, microarray-based
analyses and multiplex protein
analysis. These have a great potential
to impact future set up for clinical
diagnostics of cancer, cardiovascular
diseases, inherited diseases and com-
municable diseases, since significantly
more information can be provided by
these technologies for each patient or
sample.
Facilities
Clinical Biomarkers
clinicalbiomarkers@scilifelab.uu.se
Clinical Genomics
valtteri.wirta@scilifelab.se
Clinical Sequencing
johan.rung@scilifelab.uu.se
richard.rosenquist@igp.uu.se
Integrative Clinical Genomics
tommy.martinsson@gu.se
Translational and Clinical Genomics
ake.borg@med.lu.se
thoas.fioretos@med.lu.se
Next-Generation Diagnostics
The Structural Biology platform
provides expertise and services in the
area of biomolecular structure-func-
tion-dynamics relationships.
Cryo-EM has two sites: one special-
ized in cryo-tomography and one
specialized in single-particle cryo-
EM. Both sites have a high-end 300
kV electron microscope, equipped
with direct electron detectors.
Protein Science provides recombinant
protein production and outside the
national services also instrumentation
for crystallography and biophysical
characterization.
Swedish NMR Centre provides access
to state-of-the-art NMR spectrome-
ters and support for structure/dynam-
ics/interaction analyses of biological
macromolecules.
Facilities
Cryo-EM
gunnar.von.heijne@scilifelab.se
linda.sandblad@umu.se
Protein Science
helena.berglund@ki.se
Swedish NMR Centre (SNC)
goran.karlsson@nmr.gu.se
Structural Biology

10. The regional facilities are funded
from other sources than the national
infrastructure grant. However, these
facilities offer service and technolo-
gies for researchers in all of Sweden
within diverse areas such as bioimag-
ing, array technology, bioinformatics,
zebrafish models, biomaterials, muta-
tion analysis and mass spectrometry.
Facilities
Advanced Mass Spectrometry
Proteomics
dorothea.rutishauser@ki.se
Array and Analysis Facility
anders.isaksson@medsci.uu.se
Bioinformatics and Expression Analysis
(BEA)
fredrik.fagerstrom-billai@ki.se
Biological Visualization (BioVis)
dirk.pacholsky@scilifelab.uu.se
BioMaterial Interactions (BioMat)
marjam.ott@angstrom.uu.se
Clinical Proteomics Mass
Spectrometry
proteomicscore@scilifelab.se
Mass Spectrometry-based Proteomics,
Uppsala
margareta.ramstrom@kemi.uu.se
Mutation Analysis Facility (MAF)
kristina.duvefelt@ki.se
Zebrafish
johan.ledin@scilifelab.uu.se
Regional facilities of national interest

11. • Established in 2010 and became a national resource in 2013
• Hosted by four universities; Karolinska Institutet, KTH Royal Institute
of Technology, Stockholm University and Uppsala University, and
collaborates with many other Swedish universities
• Comprises more than 400 facility employees and 150 research
groups
• Contributed to more than 3 800 research projects during 2015
• Published more than 600 scientific articles in 2015
SciLifeLab in numbers
www.scilifelab.se