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Blood
Have you ever had a blood test? ,[object Object],[object Object],[object Object]
 
How do we see what’s in our blood? Magnification Staining
Magnification . ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Example   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Definitions - record ,[object Object],[object Object],[object Object]
e
What is a differential stain? ,[object Object],[object Object]
Spot the difference! More info to follow in lesson 4
Using a haemocytometer
 
The haemocytometer   ,[object Object],[object Object]
[object Object],[object Object],[object Object]
[object Object]
 
 
Counting cells and the Maths ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The North-west rule ,[object Object],[object Object],[object Object]
 
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Have a go!! ,[object Object]
Microscopy The light microscope v the electron microscope
Optical microscope Light microscope allows you to see the position of the cell membrane, cytoplasm, and nucleus in eukaryotic cells. Small organelles can not be seen clearly.
 
Light Microscopy:   This is the oldest, simplest and most widely-used form of microscopy. Specimens are illuminated with light, which is focussed using glass lenses and viewed using the eye or photographic film.  Specimens can be living or dead, but often need to be stained with a coloured dye to make them visible. Many different stains are available that stain specific parts of the cell such as DNA, lipids, cytoskeleton, etc. All light microscopes today are  compound microscopes , which means they use several lenses to obtain high magnification. Light microscopy has a resolution of about 200 nm, which is good enough to see cells, but not the details of cell organelles.
Magnification  is how much bigger a sample appears to be under the microscope than it is in real life. ,[object Object],[object Object],Overall magnification = Objective lens x Eyepiece lens
 
 
How do they work? ,[object Object],[object Object]
There are two types … ,[object Object],[object Object]
TEM ,[object Object],[object Object],[object Object]
Transmission EM
 
 
SEM ,[object Object],[object Object],[object Object],[object Object]
Scanning EM
Scanning EM
 
Advantages ,[object Object],[object Object],[object Object]
Disadvantages ,[object Object],[object Object],[object Object]
 
LEUCOCYTES
 
Basic information ,[object Object],[object Object]
Granulocytes
Why are they called granulocytes? ,[object Object],[object Object],[object Object],They all have small granules in the cytoplasm
Lymphocytes
Lymphocytes ,[object Object],[object Object],[object Object],[object Object]
Monocytes
Monocytes ,[object Object],[object Object],[object Object],[object Object]
What are these? Giant sized Normal sized PLATELETS- initiate blood clotting
Cell structure – beyond light microscopy
 
 
We are examples of Protista!
We are .....
We are ...
Plasma membrane Cytoplasm Nucleus EUKARYOTIC
Animal cell
Cell wall Cell membrane Vacuole Nucleus Chloroplast Starch grain EUKARYOTIC
Plant cell
 
 
The Plasma Membrane ,[object Object],[object Object]
Structure of the plasma membrane ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
The Plasma Membrane
[object Object],[object Object],[object Object]
[object Object],[object Object]
The role of the membranes ,[object Object],[object Object],[object Object],[object Object]
 
Cell membrane at approx 240 000 x magnification (electron microscope)
Cell membrane at a lower magnification Lighter area = extracellular space
How the membrane becomes more fluid at higher temperatures
Cholesterol
Cholesterol – its function. ,[object Object],[object Object],[object Object]
Extension ,[object Object],[object Object],[object Object]

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