This document discusses differential diagnosis of brucellosis serologic reactions. It summarizes that Brucella species differ in their primary smooth lipopolysaccharide antigens, with B. melitensis and B. abortus expressing A and M antigens, and B. suis expressing primarily A antigens. Immune response to Brucella infection involves both cell-mediated and humoral antibody responses. Common serologic assays used for brucellosis diagnosis include detection of antibodies in serum or milk through rapid and inexpensive tests, though vaccination and environmental conditions can impact test sensitivity and specificity.
pumpkin fruit fly, water melon fruit fly, cucumber fruit fly
Differential diagnosis of brucellosis serologic reactions
1. Differential Diagnosis of
Brucellosis Serologic Reactions
Steven G. Hennager
Bacterial and Hemoparasitic Serology Team
National Veterinary Services Laboratories
Workshop: An Integrated Approach to Controlling Brucellosis in Africa, Addis
Ababa, 29-31 January 2013
Safeguarding Animal Health
2. Dominant Brucella Antigens
Species
• Brucella melitensis
• Brucella abortus
• Brucella suis
• Brucella canis
• Brucella ovis
Primary Antigen
• M and A
• A and M
• A and M
• Rough
• Rough
5. Safeguarding Animal Health
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6. Safeguarding Animal Health
Serologic Assays
Antibody detection
Serum
Milk
Rapid
Inexpensive
Sensitivity and Specificity
Assays can be impacted by
Vaccination
Environmental conditions
Brucella card
test
15. Brief History
19th century – Bruce isolated organism
from human spleens
Linked organism to raw goat’s milk
Genus named after him
19th century – Bang discovered
organism that caused abortions in
cattle
Named organism Bacillus abortus
20th century – Evans recognized the
relationship between Bruce’s organism
and Bang’s organism
Advocated pasteurization of milk
Re-named organism Brucella abortus
Sir David
Bruce
L.F. Bernhard
Bang
Alice C. Evans