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Biosciences eastern and central Africa (BecA)




Biosciences eastern & central Africa (BecA) Hub
International Livestock Research Institute (ILRI), Nairobi, Kenya
BecA Hub: Core activities
1.   Research
     Core competencies and research programs in agriculture:
     crop, animal health and microbial sciences
2.   Capacity building and training
3.   Research and Technology-related services
4.   Focal point for the agricultural research community in
     eastern and central Africa
5.   Promotion of product development and delivery
Laboratory facilities for the Hub
Seven laboratories to provide for livestock, crop and microbial
research and training.
Laboratory upgrade and construction:
BecA Hub Core competencies
•   Genomics/Metagenomics
•   Functional genomics
•   Bioinformatics
•   Genetic engineering
•   Diagnostics
•   Molecular breeding (marker development and
    application)
•   Proteomics
•   Vaccine technology/Immunology
•   Vectors (e.g. ticks)
•   Mycotoxins
Partnerships                   2011


                        2010


                     2009


                 2008


              2007


          2006


       2005                      18(+) supported countries:
                                 Universities
                                 Nat’l Ag Research Systems (NARS)
  2004                           Research Institutions

                                 Nodes:
                                 University of Buea, Cameroon
2003                             Ethiopian Institute of Agricultural Research
                                 Sokoine University of Agriculture, Tanzania
                                 National Agricultural Research                  Rural Development
                                 Organization, Uganda                           Administration of the
                                                                                  Republic of Korea
                                                                                                        and more…
                                 Kigali Institute of Technology, Rwanda
Current BecA Hub Major Funding Agreements

• Syngenta Foundation for Sustainable Agriculture
  (USD 5M; 2009-14)

• The BecA-CSIRO partnership is part of the Australia/Africa Food
  Security Initiative
  (AUD$ 14M; 2009-13): AusAID

• The Bill & Melinda Gates Foundation core support to BecA Hub
  (USD 2M, 2011-14)

• The Swedish Ministry of Foreign Affairs/SIDA (USD12M, 2012-
  2015)

• In addition to many other investors supporting our partners,
  graduate students, etc.
SFSA Partnership

              SFSA         BecA Hub


     Staff salaries             Core support

       Emphasis:                 Capacity building
  providing affordable                Workshops
access to African users,      Technical support to Hub
promoting African –led          Institutional Support
  projects at Hub, and          African Biosciences
 product development          Challenge Fund (through
                               salaries/core support)
BecA-CSIRO Partnership

                     AusAID                      CSIRO             BecA Hub


                              Research Projects                   Capacity building
                                                                       through
                                                                 African Biosciences
 Animal health R&D




                        PPR          Aflatoxin     Amaranth
                                                                   Challenge Fund
                        ASF          Food & Nutrition Science   • Courses and
                                                                  workshops
                                    Mushrooms
                                                    Domestic    • Visiting Scientists
                       CBPP                          cavies     • Institutional Capacity
                                                                  Building

CSIRO/Australian scientific collaboration, co-
investment and partnership management                             Core support
BMGF Partnership

           BMGF       BecA Hub



                             Capacity building
     Key staffing/                through
                            African Biosciences
     core support
                              Challenge Fund
       Genomics            • Courses and
     Bioinformatics          workshops
     Crop Breeding         • Visiting Scientists
                           • Institutional Capacity
                             Building
Swedish Partnership
  Ministry for
 Foreign Affairs
                                       SIDA           BecA Hub


             Research Projects                       Capacity building
                                                          through
               Goat improvement
                                                    African Biosciences
 Molecular diagnostics: crop and animal diseases      Challenge Fund
                                                   • Courses and
       Plant tissue culture & virus indexing         workshops
                                                   • Visiting Scientists
                     staffing                      • Institutional Capacity
                                                     Building


                                                         Bioinformatics
            Core support                            platform enhancement
Research
ILRI Biotech Research
    ILRI Biotech Theme Research

Major foci
Animal health
Livestock genetics
Pathogen discovery

Research includes:
CBPP
BREAD projects
ASF
PPR
ECF
AVID
Hosted Institution Crop Research
BecA Hub
Research with Partners
      Highlights
CAAREA: Activities to date
Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA)
Aflatoxin research – critical gaps

Potential Intervention Points:
Pre-harvest: varieties (eg, KARI 170 to date)
Insect damage/resistance
Biocontrol
Drying
Storage
Binding clay
Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA)


CAAREA objectives
1.    Establish mycotoxin diagnostics platform at BecA
2.    Characterize Aspergillus flavus from around Kenya and Tanzania
      (maize and soil): as source of inoculum, resolve key population
      biology/pathosystem questions, etc.
3.    Test modeling as a potential predictive tool and to contextualize
      findings across Kenya, Tanzania and the region.
4.    Identify maize germplasm resistant to aflatoxin accumulation in
      specific environments (field trials and postharvest
      experiments), including GxE(xM).
5.    National breeders leading field trials will affect subsequent
      changes to Kenyan and Tanzanian maize breeding programs.
Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA)


Projected impact:

In Kenya alone, it is estimated that over 5.6 million people in drought prone areas in
Eastern and coastal lowlands alone will directly benefit from maize varieties with reduced
mycotoxin accumulation.

In Tanzania, not only does maize provide 60% of daily dietary calories and about 50% of
protein, but it is also a cash crop for 85% of the current Tanzania’s the population which is
estimated at about 41 million.

Platform will extend the impact by enhancing capacity to address mycotoxins in the
region.
CAAREA partners
Kenya:
BecA Hub at ILRI: Jagger Harvey (Project Leader, Research Scientist); Benoit
  Gnonlonfin (postdoc); Samuel Mutiga (Cornell PhD student); Eric Magembe;
  Vincent Were
KARI: James Gethi and team, National Maize Research Coordinator
University of Nairobi: Sheila Okoth

Tanzania:
ARI: Arnold Mushongi, National Maize Breeder
Open University of Tanzania: Said Massomo

Australia:
Ross Darnell, biometrician, CSIRO
Mary Fletcher, natural product organic chemist, QAAFI/Univ. Queensland
Glen Fox, NIR expert, QAAFI/Univ. Queensland
Darren Kriticos, ecological modeler, affiliated with CSIRO and Harvest Choice

USA:
Rebecca Nelson, Cornell University
Michael Milgroom, Cornell University
Phil Pardey, University of Minnesota, Harvest Choice

Other linked scientists:
Yash Chauhan, DEEDI (APSIM modeling for predicting aflatoxin risk)
Stephen Trowell, CSIRO (electronic nose development as a potential aflatoxin
detection method)
Harnessing husbandry of domestic cavies for alternative and rapid
               access to food and income (Cameroon and Eastern DRC)

Justification: Domestic cavies and other short cycle alternative livestock have great potential to
    contribute to addressing food security challenges in developing countries.

Objectives:
1. Characterize current production systems & establish cavies production innovation platforms.
2. Generate and integrate genetic diversity data with other breeding information to design a sustainable
   cavies production system.
3. Improved feed system for higher cavies productivity.
4. Information dissemination and capacity building.

Partners:
ILRI/BecA Hub: Appolinaire Djikeng (PI), Mwai Okeyo, Francis Wamonje
CIAT (International Center for Tropical Agriculture)
Cameroon: University of Dschang and Min of Livestock, Heifer Prog International, Farmers’ Voice
DRC: Universite Evangelique en Afrique, Women for women
Australia: CSIRO

Pathway to impact: Improved husbandry practices will be disseminated for a sustainable cavies
production linked to market and consumption.
Domestication of wild edible mushrooms in E. Africa (Nat’l Program-Led)

Justification: Income generation.

Objectives:
1. Collect and characterize wild edible mushrooms.
2. Domesticate them on agro-wastes (eg, sisal, rice straw).
3. Nutritionally profile domesticated varieties.
4. Farmer training.

Partners:
University of Dar es Salaam Prof. Amelia Kivaisi (PI)
Kenya Industrial Research and
Development Institute (KIRDI)
University of Burundi
CSIRO
BecA Hub at ILRI

Pathway to impact:
Existing mushroom domestication and training programs at each institution will be expanded
to include these indigenous varieties.
Amaranth (Nat’l Program Led)

Justification: Amaranth is popular (vegetable & grain) in many African countries.

Objective:
Reduce food and nutrition insecurity in rural communities of SSA by increasing amaranth
production & transforming its leaves and grain products into a variety of shelf stable,
nutritious, highly acceptable and marketable products.

Partners:
Jomo Kenyatta University of Agriculture and Technology
          Dr Daniel Sila (PI)
Sokoine University of
AVRDC (Tanzania)
CSIRO
BecA Hub at ILRI

Pathway to impact:
Stakeholder consultations & training through established extension activities at the African
institutions will be used. Amaranth is very popular in these countries.
PPR
   Development of Improved Control Interventions for Peste des Petits Ruminants (PPR)

Justification: PPR is an important disease of small ruminants with challenges in vaccine
    delivery (cold chain,…).

Objectives:

1: To thermostabilize existing PPR vaccine
     Nigeria 75/1 strain - OIE recommended
     Widely used in Africa

2: To pilot vaccine delivery models

Partners:
BecA-ILRI Biotech
Au-IBAR
Australia

Pathway to impact:
Research embedded in development (ILRI and AU-IBAR) and piloting of vaccination/new
institutional models will help ensure delivery of an improved vaccine.
The BecA Hub:
A key driver for Agricultural transformation

 LOCATION: well positioned to co-operate with regional and
 international partners.


 CAPACITY: build a critical mass of scientists by training and
 engaging them in a wide range of research activities.


 SCIENCE: focused on research that can address pressing
 constraints for Africa’s smallholder farmers.
Research-related services at the BecA Hub and their
     impact on research and capacity building
BecA Hub: Core activities



1.   Research

2.   Research-related services
3.   Capacity building and training
4.   Focal point for the agricultural research community in
     eastern and central Africa
5.   Promotion of product development and delivery
Presentation outline


I.     The Central Core Unit

II.    The SEGOLIP Unit

III.   The Genomics platform

IV.    The Bioinformatics platform

V.     Other platforms
I. Central Core Unit


Staff (10)
• 01 Manager
• 03 Tech Lab Ass
• 06 Lab cleaners

Current services
• Laboratory cleaning and waste management
• Glassware cleaning and sterilization services
• Preparation of culture media and buffers
• Mol Biol grade water, custom made solutions
I. Central Core Unit (Cont’d)

Users/customers
• BecA Hub staff and all African researchers using the Hub
• ILRI Biotech (media/buffers and packaging of the ITM vaccine)
• Hosted institutions (IITA, CIMMYT, CIP)
•   Kenya Agricultural Research Institute (KARI)
•   Kenya Medical Research Institute (KEMRI)
•   Institute for Primate Research (IPR)
•   Universities (Univ of Nairobi, JKUAT, KU, MMU, Egerton Univ)
•   icipe
•   Other CG centers based in Nairobi
•   Syngenta Flowers
• Makerere University, Uganda
• MARI, Tanzania
I. Central Core Unit (outlook)




Expansion to other users/customers)
• Other institutions in the region (Hub activities’ expansion)

Other services under consideration (include)
• Molecular biology reagents (nucleic acids extraction reagents,
  DNA size standards)
• Enzymes (i.e. Taq DNA polymerase)
II. SEGOLIP Unit


Staff
04 technical staff
Extensive lab experience

Current Services
a.      DNA sequencing
        Sanger sequencing (capillary – low to medium
throughput)
        Pyrosequencing (next generation – high throughput)
b.      Genotyping
        Full genotyping 01 (DNA extraction, PCR, fragment
analysis)
        Full genotyping 02 (PCR, fragment analysis)
        Partial genotyping (fragment analysis)
II. SEGOLIP Unit (Cont’d)
             Supporting a wide range of research projects


   Banana
   Beans      Arthropod
  Cassava      vectors
  Cowpea       Buffalo
 Eucalyptus    Chicken
    Maize        Cow
   Millets       Goat
Napier grass     Pigs
  Ocimum        Sheep
Passion fruit    Mice
 Pigeon pea    Wildlife
     Rice
  Sorghum
    Striga     Bacteria
 Sugar cane     Fungi
Sweet potato Parasites
      Tef      Viruses
     Yam
II. SEGOLIP Unit (Cont’d)
                   Supporting a wide range of programs

1. Generation Challenge Program (GCP): 2011 Work Order
• 09 countries (South Africa, The Philippines, Kenya, Ghana, Ethiopia,
   Uganda, India, Burkina Faso, Mexico)
• 10 crops (maize, rice, sorghum, cowpea, chickpeas, cassava, sweet
   potatoes, beans, finger millet, pearl millet)

2. Projects at the Hub facilities
• Support (services and training) to all ABCF fellows, AWARD fellows,
   graduates students and other visiting scientists projects
• ILRI Biotech Theme
• Hosted CGIAR crop centers

3. Other users (African NARS, universities, international research institutes
and regional programs, USA, Latin America, Middle East and Asia)
II. SEGOLIP Unit (Cont’d)
           supporting various breeding programs in Africa

1. Characterization of sorghum germplasm collection focusing on biotic and
abiotic stresses (Kassahun Bante – Jimma University, Ethiopia)

2. Stress Tolerant Rice for Africa and South Asia (STRASA) project: Development
and delivery of improved rice varieties that are tolerant to five major abiotic
stresses; drought, submergence, salinity, iron toxicity and low temperature
(Negussie Zena – Africa Rice Center)

3. Cassava breeding in Uganda (Robert Kawuki, NARO, Uganda)

4. Genotyping of sorghum BC4F1 population associated with striga resistance
(Rasha Ali – University of Khartoum, Sudan)

5. Genetic diversity to support a goat breeding program in Cameroon (Felix
Meutchieye – University of Dschang, Cameroon)

6. Genetic characterization of Senegalese trypanotolerant N’Dama cattle (Mame
Diouf – ISRA, Senegal)
II. SEGOLIP Unit (Cont’d)
                              A steadily increasing demand
                        DNA SEQUENCING SERVICE PER YEAR
                8000


                7000


                6000


                5000
No of Samples




                4000
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                2000


                1000


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IV. The Genomics platform
      Opportunities for genomics and metagenomics research


                        ABI 3130-xl     ABI 3730-xl              ABI 3500-xl

Capillary sequencing




                                          1 sample = 1 library
                                                   = 1 plate
   Next generation                        500 mb/run
     sequencing:                          1/2 cassava genome
                                          1/8 human genome
454 GS pyrosequencer
IV. The Genomics platform (Cont’d)
                      Highlights of applications



Genomics (microbial and other organisms)
1. Large genomes re-sequencing (Cassava)
2. Viral genomics (African Swine Fever, Rift Valley Fever, blue tongue
   virus, equine encephalitis virus)
3. Functional genomics (small viral RNAs in cassava)

Metagenomics
1. Pathogen discovery, tracking and surveillance of zoonotic diseases
   (e.g. RVF)
2. Microbiome analysis; environmental metagenomics (e.g. aquatic
   environment)
IV. The Genomics platform (Cont’d)
                         Major outputs



Completed and ongoing projects
1.    Re-sequencing of the cassava genome
      # 06 next gen sequencing runs => 1.4 billions bases =>
      ~ 1.5x coverage of the cassava genome
      # Ongoing data analysis (BecA Hub/IITA, Univ Arizona)
2.     Deep sequencing to support a study on soil management
       (Study on the interaction between resident and inoculated
       mycorrhizal communities) – TSBF-CIAT
       # ¼ next gen sequencing run with 36 multiplexed samples
       # Ongoing data analysis (BecA Hub, TSBF-CIAT)
IV. The Genomics platform (Cont’d)
                              Major outputs


3.      Pathogens genomes sequencing projects
        (61 viral genomes sequenced so far with 14 deposited @ Genbank)

     # Rift valley fever virus (16 sequenced and ongoing analysis)
     # New castle disease virus (03 sequenced and deposited @ Genbank)
     # African swine fever virus (02 sequenced and ongoing analysis)
     # Equine encephalitis virus (05 sequenced and ongoing analysis)
     # Blue tongue virus; collection 1970 – now (24; ongoing sequencing)
     # Ndumu Virus (07 partially sequenced) // also found in pigs (discovery
     !!)
     # Other viruses: Dugbe virus (01), semliki virus (02), bunyamwera (01);
     deposited @ Genbank
V. The Genomics platform (Cont’d)
                    Highlights of forthcoming projects

Genomics:
# Genome sequencing of napier stunt disease pathogen, phytoplasma (icipe, ILRI)
# Genomics of Novel Respiratory Adenovirus isolates (CDC, Kenya)
# Genomic characterization of hMPV isolates in of Kenya (Walter Reed, Kenya)
# Heredity and Human Health in Africa (H3 Africa – sequencing and genotyping
support), Wellcome Trust and NIH
# Development of new markers (Enset, etc..)

Metagenomics:
# Microbial community in selected environments of Nairobi city district (Univ Notre
Dame, ILRI)
# “Viromes” of selected African farming systems; assessing food security risks
(BecA, KARI, FERA)
# Heredity and Human Health in Africa (H3 Africa – sequencing support)
IV. The Bioinformatics platform




• Linux cluster
   • 32 CPUs (AMD 64-bit)
   • 128 Gigabyte RAM
• >10 terabytes disk storage
• Grid computing
• Parallel applications:
   > Genome assembly (Newbler,
      MIRA, Celera, velvet, CAP3. …)
   > Genome annotation (glimmer, …)
   > Phylogenetic analysis (Beast, Mr   IRRI – Philippines
                                                                        BecA/ILRI
      Bayes)                            ICRISAT – India
   > Other sequence analysis tools      CIP – Peru
      (BLAST, clustalw, HMMER, R)
                                                       Bioinformatics Group Leader
                                                       Dr Etienne de Villiers
IV. The Bioinformatics platform (Cont’d)
                            Selected outputs


1. Research support
   # Genomics approach to the identification of virulence genes of CBPP
   # Genomics approach to the development of vaccines and diagnostics
   of camel Streptococcus agalactiae (to support camel milk marketing
   through improved control of mastitis)
   # Cassava genome project
   # Molecular markers development
   # Diagnostic development (ex. Passion fruit)

2. Capacity building
   # Since 2006: 15 bioinformatics courses (~ 391 participants from
   African institutions)
   # Established a Regional Student Group (RSG) affiliated with The
   International Society for Computational Biology (ISCB) in 2007
VI. Other platforms
         Expanding our research and capacity building
                 and services opportunities

1.   Diagnostics platform (from sequence to impact): exploiting
     genomic data

     Animal and zoonotic diseases

     Crop pathogens:
     Viral diseases
     Bacteria
     Ralstonia solanacearum (Endemic disease - keep this out of
     local seed)
     Dickeya solani (European disease - keep this out of the region)
VI. Other platforms
         Expanding our research and capacity building
                 and services opportunities

2. Mycotoxin and nutrition analysis platform
   Infrastructure:
   Atomic Absorption Spectrophotometer
   Gas chromatograph – Mass Spectrophotometer (GC-MS)
   HPLC system comprising UHPLC solvent system
   Fourier Transform Near Infrared (FT-NIR) spectrometer

  Applications/measurements:
  Mycotoxins, sugars, vitamins, carotenoids, phenolics, fatty acids
  and amino acids, trait analysis (crops and animals)
VI. Other platforms (Cont’d)
            Expanding our research, capacity building
                   and service opportunities

3. Online data integration and analysis platforms

  iPlant Collaborative (Cyberinfrastructure to support plant biology
  research.
  # Sustainable access to high performance computing,
  interoperable software analysis, and large data sets

  ibp (Integrated breeding platform: public web-based one-stop
  shop for information, analytical tools and related services to
  design and efficiently conduct molecular-assisted breeding
  experiments)
Strategies for new equipment and technologies acquisition
Strategies for new equipment and technologies acquisition
                   (Some challenges!!!)



 BecA Hub Lab asset distribution
                                                  >100K (30%)

                                                  5OK-100K (10%)

                                                  10K - 50K (30%)

                                                  < 10K (30%)




Equipment replacement plan
        # < 100 000 USD (Grants)
        # > 100 000 USD (special donations)
        # Capital expenditure ???
Thank you




Biosciences eastern & central Africa (BecA) Hub
International Livestock Research Institute (ILRI), Nairobi, Kenya
http://hub.africabiosciences.org
The BecA Hub Capacity Building Programme
“Biosciences are seen as one of the major engines of
  growth in the world in fields such as …. agriculture.
  Africa lags behind in biosciences. The two key
  problems are lack of sufficient funding from
  governments and shortage of skilled expertise.”

  NEPAD www.nepad.org/humancapitaldevelopment/abi
Objectives

• Strengthen capacity of individuals and institutions to harness
  the latest biosciences technologies to improve agriculture in
  Africa

• Support African scientists efforts to lead and sustain
  biosciences research in Africa

• Promote access to world-class
  research and training facilities at
  the BecA Hub
Building biosciences capacity for improving African agriculture



   1. Research placements
        • Graduate students
        • Visiting scientists
   2.   Individual/small group training
   3.   Training workshops
   4.   Conferences
   5.   Institutional capacity building
   6.   Linkages, information, creating awareness
        of BecA Hub
Growing numbers using the Hub
Graduate students, visiting scientists and short term trainees
Individual/small group training



•   1-5 trainees
•   1-4 weeks
•    Customised training
•   One or more technologies
Training workshops

• Hosted by the BecA Hub
   – 2007-2011: 42 training workshops
   – Examples (2011):
       • Basic Laboratory Health and Safety (ILRI, BecA)
       • Marker Assisted Breeding (ICRISAT)

• Annual practical training workshops organised by BecA
  Hub, under ABCF
  1.   Science paper writing
  2.   Introduction to molecular biology and bioinformatics
  3.   Advanced bioinformatics
  4.   New for 2012: Laboratory management & equipment
       maintenance
Science Writing Workshops: 2009 - 2011


• Applications: 703 (2009), 560 (2011)
• 64 participants from 15 African countries (27 women,
37 men)

• Outputs (2009, 2010): 24 papers submitted, 15
published

• 2009: BMGF funding; training by Scriptoria
• 2010, 2011: AusAID funding; training led by CSIRO

           “I had been struggling for one year with my
           introduction, but I finished it in one afternoon.”
           Anne Akol, Senior Lecturer, Makerere University,
           Uganda
Molecular Biology and Bioinformatics
          Workshop 2011


• 21 participants (6 women, 15 men)
• 10 African countries

• Research discovery process
               Tissue
               DNA
               PCR
               Cloning
               Sequencing              BLAST
               Sequence analysis
Advanced bioinformatics workshop 2011:
     Next Generation Sequencing for Africa

• 24 participants (18 men, 6 women) from Africa

• 13 tutors from Kenya, Europe, USA

• EMBO Global Exchange Lecture Course:
  Next Generation Sequencing data analysis

• 4 collaborations initiated
        - RVFV (VRI-Sudan, ILRI-BecA)
        - Finger millet SNPs (KU-Ke, U Liverpool)
        - Trypanosome MDR (SUA-Tz, U Liverpool)
        - Drought stress in banana (Makerere U-Ug, BecA)
Building capacity through research

• Major focus
  – Post graduate research
    projects (up to 3-4 yrs)
          – Students registered at many
            universities
  – Visiting scientist placements
    (up to 6 months)
          – Employees from NARIs and
            Universities



ABCF: African Biosciences Challenge Fund
Making the ABCF possible


• Syngenta Foundation for Sustainable Agriculture (SFSA)

• BecA-CSIRO partnership funded by the Australian Agency
  for International Development (AusAID)

• The Bill & Melinda Gates Foundation (BMGF)

• Ministry for Foreign Affairs, Sweden, through the
  Swedish International Development Cooperation Agency
  (SIDA)
ABCF Research Fellowships
• Large demand for use of BecA Hub: inadequate funds
• Enable African scientists access Hub facilities and services, for
  high quality research addressing African agricultural problems
• Researchers from national research institutes and African
  universities
• 3-6 months at the Hub
• Competitive basis or targeted ‘fast track’
ABCF Research Fellows
Charles Masembe
Department of Zoology, Makerere University, Uganda

Pig diseases and food security: Next-generation DNA
sequencing of African swine fever virus (ASFV) in Uganda




Selamawit Bedane (Haramaya University, Ethiopia)
Sisay Alemu (Holetta Agricultural Research Center, EIAR, Ethiopia)

Molecular characterization of enset from Ethiopia using
banana microsatellite markers
ABCF Research Fellows
Dia Hassan
Central Veterinary Research Laboratories, Khartoum, Sudan

Theileria parva genotyping to support control of East Coast
fever, an emerging disease in South Sudan




 Dora Kilalo
 Department of Plant Science and Crop Protection, University of Nairobi

 Passion fruit woodiness disease diagnostics
ABCF Research Fellows

Alexander Bombom
Department of Agricultural Production, Makerere University

Molecular characterization of maize-sorghum hybrids




 Félix Meutchieye
 University of Dschang, Cameroon

 Molecular characterization of Cameroon indigenous goats and
 sheep
Building capacity of African institutes

• National Agricultural Biotechnology
  Centre, Holetta, Ethiopia
• Gulu University, Uganda
• Mikocheni Agricultural Research
  Institute (MARI), Tanzania
Raising awareness
38 institutes and organisations in 8 countries visited in 2011
  •   Burundi
  •   Cameroon
  •   Ethiopia
  •   Rwanda
  •   Sudan
  •   Tanzania
  •   Uganda
  •   Nigeria

  •   Conferences in many countries
  •   BecA workshops
  •   Website
  •   Printed materials
  •   We host over 1500 visitors per year
BecA alumni: Where are they now?




                                   IRRI
Integration of research, capacity building and research-
                    related services
          For a better delivery of the BecA mission




                        Research




             Research
                                    Capacity
              related
                                    building
             services
Acknowledgements
• Australian Agency for International Development (AusAID)/CSIRO
• Bill & Melinda Gates Foundation (BMGF)
• Ministry for Foreign Affairs, Sweden, through the Swedish International
  Development Cooperation Agency (SIDA)
• Syngenta Foundation for Sustainable Agriculture (SFSA)
• NEPAD/AU
• Canadian International Development Agency (CIDA)
• Google Foundation
• Rockefeller Foundation
• Gatsby Charitable Foundation
• Doyle Foundation
• The Kenyan Government
• and many others
Thank you




     IRRI

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BecA Hub (training, lab support)Pathway to impact:- Mushroom farming as an alternative livelihood.- Nutritional security.- Income generation.- Training manuals and farmer field schools.Potential for regional expansion

  • 1. Biosciences eastern and central Africa (BecA) Biosciences eastern & central Africa (BecA) Hub International Livestock Research Institute (ILRI), Nairobi, Kenya
  • 2. BecA Hub: Core activities 1. Research Core competencies and research programs in agriculture: crop, animal health and microbial sciences 2. Capacity building and training 3. Research and Technology-related services 4. Focal point for the agricultural research community in eastern and central Africa 5. Promotion of product development and delivery
  • 3. Laboratory facilities for the Hub Seven laboratories to provide for livestock, crop and microbial research and training. Laboratory upgrade and construction:
  • 4. BecA Hub Core competencies • Genomics/Metagenomics • Functional genomics • Bioinformatics • Genetic engineering • Diagnostics • Molecular breeding (marker development and application) • Proteomics • Vaccine technology/Immunology • Vectors (e.g. ticks) • Mycotoxins
  • 5. Partnerships 2011 2010 2009 2008 2007 2006 2005 18(+) supported countries: Universities Nat’l Ag Research Systems (NARS) 2004 Research Institutions Nodes: University of Buea, Cameroon 2003 Ethiopian Institute of Agricultural Research Sokoine University of Agriculture, Tanzania National Agricultural Research Rural Development Organization, Uganda Administration of the Republic of Korea and more… Kigali Institute of Technology, Rwanda
  • 6.
  • 7. Current BecA Hub Major Funding Agreements • Syngenta Foundation for Sustainable Agriculture (USD 5M; 2009-14) • The BecA-CSIRO partnership is part of the Australia/Africa Food Security Initiative (AUD$ 14M; 2009-13): AusAID • The Bill & Melinda Gates Foundation core support to BecA Hub (USD 2M, 2011-14) • The Swedish Ministry of Foreign Affairs/SIDA (USD12M, 2012- 2015) • In addition to many other investors supporting our partners, graduate students, etc.
  • 8. SFSA Partnership SFSA BecA Hub Staff salaries Core support Emphasis: Capacity building providing affordable Workshops access to African users, Technical support to Hub promoting African –led Institutional Support projects at Hub, and African Biosciences product development Challenge Fund (through salaries/core support)
  • 9. BecA-CSIRO Partnership AusAID CSIRO BecA Hub Research Projects Capacity building through African Biosciences Animal health R&D PPR Aflatoxin Amaranth Challenge Fund ASF Food & Nutrition Science • Courses and workshops Mushrooms Domestic • Visiting Scientists CBPP cavies • Institutional Capacity Building CSIRO/Australian scientific collaboration, co- investment and partnership management Core support
  • 10. BMGF Partnership BMGF BecA Hub Capacity building Key staffing/ through African Biosciences core support Challenge Fund Genomics • Courses and Bioinformatics workshops Crop Breeding • Visiting Scientists • Institutional Capacity Building
  • 11. Swedish Partnership Ministry for Foreign Affairs SIDA BecA Hub Research Projects Capacity building through Goat improvement African Biosciences Molecular diagnostics: crop and animal diseases Challenge Fund • Courses and Plant tissue culture & virus indexing workshops • Visiting Scientists staffing • Institutional Capacity Building Bioinformatics Core support platform enhancement
  • 13. ILRI Biotech Research ILRI Biotech Theme Research Major foci Animal health Livestock genetics Pathogen discovery Research includes: CBPP BREAD projects ASF PPR ECF AVID
  • 15. BecA Hub Research with Partners Highlights
  • 16. CAAREA: Activities to date Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA)
  • 17.
  • 18. Aflatoxin research – critical gaps Potential Intervention Points: Pre-harvest: varieties (eg, KARI 170 to date) Insect damage/resistance Biocontrol Drying Storage Binding clay
  • 19. Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA) CAAREA objectives 1. Establish mycotoxin diagnostics platform at BecA 2. Characterize Aspergillus flavus from around Kenya and Tanzania (maize and soil): as source of inoculum, resolve key population biology/pathosystem questions, etc. 3. Test modeling as a potential predictive tool and to contextualize findings across Kenya, Tanzania and the region. 4. Identify maize germplasm resistant to aflatoxin accumulation in specific environments (field trials and postharvest experiments), including GxE(xM). 5. National breeders leading field trials will affect subsequent changes to Kenyan and Tanzanian maize breeding programs.
  • 20. Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA) Projected impact: In Kenya alone, it is estimated that over 5.6 million people in drought prone areas in Eastern and coastal lowlands alone will directly benefit from maize varieties with reduced mycotoxin accumulation. In Tanzania, not only does maize provide 60% of daily dietary calories and about 50% of protein, but it is also a cash crop for 85% of the current Tanzania’s the population which is estimated at about 41 million. Platform will extend the impact by enhancing capacity to address mycotoxins in the region.
  • 21. CAAREA partners Kenya: BecA Hub at ILRI: Jagger Harvey (Project Leader, Research Scientist); Benoit Gnonlonfin (postdoc); Samuel Mutiga (Cornell PhD student); Eric Magembe; Vincent Were KARI: James Gethi and team, National Maize Research Coordinator University of Nairobi: Sheila Okoth Tanzania: ARI: Arnold Mushongi, National Maize Breeder Open University of Tanzania: Said Massomo Australia: Ross Darnell, biometrician, CSIRO Mary Fletcher, natural product organic chemist, QAAFI/Univ. Queensland Glen Fox, NIR expert, QAAFI/Univ. Queensland Darren Kriticos, ecological modeler, affiliated with CSIRO and Harvest Choice USA: Rebecca Nelson, Cornell University Michael Milgroom, Cornell University Phil Pardey, University of Minnesota, Harvest Choice Other linked scientists: Yash Chauhan, DEEDI (APSIM modeling for predicting aflatoxin risk) Stephen Trowell, CSIRO (electronic nose development as a potential aflatoxin detection method)
  • 22. Harnessing husbandry of domestic cavies for alternative and rapid access to food and income (Cameroon and Eastern DRC) Justification: Domestic cavies and other short cycle alternative livestock have great potential to contribute to addressing food security challenges in developing countries. Objectives: 1. Characterize current production systems & establish cavies production innovation platforms. 2. Generate and integrate genetic diversity data with other breeding information to design a sustainable cavies production system. 3. Improved feed system for higher cavies productivity. 4. Information dissemination and capacity building. Partners: ILRI/BecA Hub: Appolinaire Djikeng (PI), Mwai Okeyo, Francis Wamonje CIAT (International Center for Tropical Agriculture) Cameroon: University of Dschang and Min of Livestock, Heifer Prog International, Farmers’ Voice DRC: Universite Evangelique en Afrique, Women for women Australia: CSIRO Pathway to impact: Improved husbandry practices will be disseminated for a sustainable cavies production linked to market and consumption.
  • 23. Domestication of wild edible mushrooms in E. Africa (Nat’l Program-Led) Justification: Income generation. Objectives: 1. Collect and characterize wild edible mushrooms. 2. Domesticate them on agro-wastes (eg, sisal, rice straw). 3. Nutritionally profile domesticated varieties. 4. Farmer training. Partners: University of Dar es Salaam Prof. Amelia Kivaisi (PI) Kenya Industrial Research and Development Institute (KIRDI) University of Burundi CSIRO BecA Hub at ILRI Pathway to impact: Existing mushroom domestication and training programs at each institution will be expanded to include these indigenous varieties.
  • 24. Amaranth (Nat’l Program Led) Justification: Amaranth is popular (vegetable & grain) in many African countries. Objective: Reduce food and nutrition insecurity in rural communities of SSA by increasing amaranth production & transforming its leaves and grain products into a variety of shelf stable, nutritious, highly acceptable and marketable products. Partners: Jomo Kenyatta University of Agriculture and Technology Dr Daniel Sila (PI) Sokoine University of AVRDC (Tanzania) CSIRO BecA Hub at ILRI Pathway to impact: Stakeholder consultations & training through established extension activities at the African institutions will be used. Amaranth is very popular in these countries.
  • 25. PPR Development of Improved Control Interventions for Peste des Petits Ruminants (PPR) Justification: PPR is an important disease of small ruminants with challenges in vaccine delivery (cold chain,…). Objectives: 1: To thermostabilize existing PPR vaccine Nigeria 75/1 strain - OIE recommended Widely used in Africa 2: To pilot vaccine delivery models Partners: BecA-ILRI Biotech Au-IBAR Australia Pathway to impact: Research embedded in development (ILRI and AU-IBAR) and piloting of vaccination/new institutional models will help ensure delivery of an improved vaccine.
  • 26. The BecA Hub: A key driver for Agricultural transformation LOCATION: well positioned to co-operate with regional and international partners. CAPACITY: build a critical mass of scientists by training and engaging them in a wide range of research activities. SCIENCE: focused on research that can address pressing constraints for Africa’s smallholder farmers.
  • 27. Research-related services at the BecA Hub and their impact on research and capacity building
  • 28. BecA Hub: Core activities 1. Research 2. Research-related services 3. Capacity building and training 4. Focal point for the agricultural research community in eastern and central Africa 5. Promotion of product development and delivery
  • 29. Presentation outline I. The Central Core Unit II. The SEGOLIP Unit III. The Genomics platform IV. The Bioinformatics platform V. Other platforms
  • 30. I. Central Core Unit Staff (10) • 01 Manager • 03 Tech Lab Ass • 06 Lab cleaners Current services • Laboratory cleaning and waste management • Glassware cleaning and sterilization services • Preparation of culture media and buffers • Mol Biol grade water, custom made solutions
  • 31. I. Central Core Unit (Cont’d) Users/customers • BecA Hub staff and all African researchers using the Hub • ILRI Biotech (media/buffers and packaging of the ITM vaccine) • Hosted institutions (IITA, CIMMYT, CIP) • Kenya Agricultural Research Institute (KARI) • Kenya Medical Research Institute (KEMRI) • Institute for Primate Research (IPR) • Universities (Univ of Nairobi, JKUAT, KU, MMU, Egerton Univ) • icipe • Other CG centers based in Nairobi • Syngenta Flowers • Makerere University, Uganda • MARI, Tanzania
  • 32. I. Central Core Unit (outlook) Expansion to other users/customers) • Other institutions in the region (Hub activities’ expansion) Other services under consideration (include) • Molecular biology reagents (nucleic acids extraction reagents, DNA size standards) • Enzymes (i.e. Taq DNA polymerase)
  • 33. II. SEGOLIP Unit Staff 04 technical staff Extensive lab experience Current Services a. DNA sequencing Sanger sequencing (capillary – low to medium throughput) Pyrosequencing (next generation – high throughput) b. Genotyping Full genotyping 01 (DNA extraction, PCR, fragment analysis) Full genotyping 02 (PCR, fragment analysis) Partial genotyping (fragment analysis)
  • 34. II. SEGOLIP Unit (Cont’d) Supporting a wide range of research projects Banana Beans Arthropod Cassava vectors Cowpea Buffalo Eucalyptus Chicken Maize Cow Millets Goat Napier grass Pigs Ocimum Sheep Passion fruit Mice Pigeon pea Wildlife Rice Sorghum Striga Bacteria Sugar cane Fungi Sweet potato Parasites Tef Viruses Yam
  • 35. II. SEGOLIP Unit (Cont’d) Supporting a wide range of programs 1. Generation Challenge Program (GCP): 2011 Work Order • 09 countries (South Africa, The Philippines, Kenya, Ghana, Ethiopia, Uganda, India, Burkina Faso, Mexico) • 10 crops (maize, rice, sorghum, cowpea, chickpeas, cassava, sweet potatoes, beans, finger millet, pearl millet) 2. Projects at the Hub facilities • Support (services and training) to all ABCF fellows, AWARD fellows, graduates students and other visiting scientists projects • ILRI Biotech Theme • Hosted CGIAR crop centers 3. Other users (African NARS, universities, international research institutes and regional programs, USA, Latin America, Middle East and Asia)
  • 36. II. SEGOLIP Unit (Cont’d) supporting various breeding programs in Africa 1. Characterization of sorghum germplasm collection focusing on biotic and abiotic stresses (Kassahun Bante – Jimma University, Ethiopia) 2. Stress Tolerant Rice for Africa and South Asia (STRASA) project: Development and delivery of improved rice varieties that are tolerant to five major abiotic stresses; drought, submergence, salinity, iron toxicity and low temperature (Negussie Zena – Africa Rice Center) 3. Cassava breeding in Uganda (Robert Kawuki, NARO, Uganda) 4. Genotyping of sorghum BC4F1 population associated with striga resistance (Rasha Ali – University of Khartoum, Sudan) 5. Genetic diversity to support a goat breeding program in Cameroon (Felix Meutchieye – University of Dschang, Cameroon) 6. Genetic characterization of Senegalese trypanotolerant N’Dama cattle (Mame Diouf – ISRA, Senegal)
  • 37. II. SEGOLIP Unit (Cont’d) A steadily increasing demand DNA SEQUENCING SERVICE PER YEAR 8000 7000 6000 5000 No of Samples 4000 * 3000 2000 1000 0 2005 2006 2007 2008 2009 2010 2011 Year
  • 38. Su # Full genotyping 01 ga rI nd us # Data 2010 – Aug 2011 tr yR es ea rc h Bi In Ke ot s Un ny ec tu ive a hn te 0 100 200 300 400 500 600 700 rs Fo ol (M ity re og au of st r yC ri Fr yR en us ee sta es ea te r( ) te rc Ec h ua Number of Samples -B In lo em s do r) tu fo te Un nt (K ive ein en rs (S ya ity ou ) of th Un Kw Af Af ive a- ric ric rsi zu aR a) ty lu i ce of Na Ce Ca ta nt lif l( er or So ni ut a- h Na Un Lo Af ric on ive sA ng a) al rs Cr ity ele op of s( Re Jim Zim US so m ba A) ur aU bw ce s ni ve e( Re rsi ZW se ty ) Ke ar (E ch th ny In io aA Eg s pi Na gr er tu a) icu to te n (U serving institutions within and out of Africa on ltu Institutions using genotyping services al ra Un ga Ro lR ive nd ot es rs a) Cr ea ity op rc (K h en sR In es s ya ea tu ) rc te h (K In en s ya tu ) te (N ige ria )
  • 39. IV. The Genomics platform Opportunities for genomics and metagenomics research ABI 3130-xl ABI 3730-xl ABI 3500-xl Capillary sequencing 1 sample = 1 library = 1 plate Next generation 500 mb/run sequencing: 1/2 cassava genome 1/8 human genome 454 GS pyrosequencer
  • 40. IV. The Genomics platform (Cont’d) Highlights of applications Genomics (microbial and other organisms) 1. Large genomes re-sequencing (Cassava) 2. Viral genomics (African Swine Fever, Rift Valley Fever, blue tongue virus, equine encephalitis virus) 3. Functional genomics (small viral RNAs in cassava) Metagenomics 1. Pathogen discovery, tracking and surveillance of zoonotic diseases (e.g. RVF) 2. Microbiome analysis; environmental metagenomics (e.g. aquatic environment)
  • 41. IV. The Genomics platform (Cont’d) Major outputs Completed and ongoing projects 1. Re-sequencing of the cassava genome # 06 next gen sequencing runs => 1.4 billions bases => ~ 1.5x coverage of the cassava genome # Ongoing data analysis (BecA Hub/IITA, Univ Arizona) 2. Deep sequencing to support a study on soil management (Study on the interaction between resident and inoculated mycorrhizal communities) – TSBF-CIAT # ¼ next gen sequencing run with 36 multiplexed samples # Ongoing data analysis (BecA Hub, TSBF-CIAT)
  • 42. IV. The Genomics platform (Cont’d) Major outputs 3. Pathogens genomes sequencing projects (61 viral genomes sequenced so far with 14 deposited @ Genbank) # Rift valley fever virus (16 sequenced and ongoing analysis) # New castle disease virus (03 sequenced and deposited @ Genbank) # African swine fever virus (02 sequenced and ongoing analysis) # Equine encephalitis virus (05 sequenced and ongoing analysis) # Blue tongue virus; collection 1970 – now (24; ongoing sequencing) # Ndumu Virus (07 partially sequenced) // also found in pigs (discovery !!) # Other viruses: Dugbe virus (01), semliki virus (02), bunyamwera (01); deposited @ Genbank
  • 43. V. The Genomics platform (Cont’d) Highlights of forthcoming projects Genomics: # Genome sequencing of napier stunt disease pathogen, phytoplasma (icipe, ILRI) # Genomics of Novel Respiratory Adenovirus isolates (CDC, Kenya) # Genomic characterization of hMPV isolates in of Kenya (Walter Reed, Kenya) # Heredity and Human Health in Africa (H3 Africa – sequencing and genotyping support), Wellcome Trust and NIH # Development of new markers (Enset, etc..) Metagenomics: # Microbial community in selected environments of Nairobi city district (Univ Notre Dame, ILRI) # “Viromes” of selected African farming systems; assessing food security risks (BecA, KARI, FERA) # Heredity and Human Health in Africa (H3 Africa – sequencing support)
  • 44. IV. The Bioinformatics platform • Linux cluster • 32 CPUs (AMD 64-bit) • 128 Gigabyte RAM • >10 terabytes disk storage • Grid computing • Parallel applications: > Genome assembly (Newbler, MIRA, Celera, velvet, CAP3. …) > Genome annotation (glimmer, …) > Phylogenetic analysis (Beast, Mr IRRI – Philippines BecA/ILRI Bayes) ICRISAT – India > Other sequence analysis tools CIP – Peru (BLAST, clustalw, HMMER, R) Bioinformatics Group Leader Dr Etienne de Villiers
  • 45. IV. The Bioinformatics platform (Cont’d) Selected outputs 1. Research support # Genomics approach to the identification of virulence genes of CBPP # Genomics approach to the development of vaccines and diagnostics of camel Streptococcus agalactiae (to support camel milk marketing through improved control of mastitis) # Cassava genome project # Molecular markers development # Diagnostic development (ex. Passion fruit) 2. Capacity building # Since 2006: 15 bioinformatics courses (~ 391 participants from African institutions) # Established a Regional Student Group (RSG) affiliated with The International Society for Computational Biology (ISCB) in 2007
  • 46. VI. Other platforms Expanding our research and capacity building and services opportunities 1. Diagnostics platform (from sequence to impact): exploiting genomic data Animal and zoonotic diseases Crop pathogens: Viral diseases Bacteria Ralstonia solanacearum (Endemic disease - keep this out of local seed) Dickeya solani (European disease - keep this out of the region)
  • 47. VI. Other platforms Expanding our research and capacity building and services opportunities 2. Mycotoxin and nutrition analysis platform Infrastructure: Atomic Absorption Spectrophotometer Gas chromatograph – Mass Spectrophotometer (GC-MS) HPLC system comprising UHPLC solvent system Fourier Transform Near Infrared (FT-NIR) spectrometer Applications/measurements: Mycotoxins, sugars, vitamins, carotenoids, phenolics, fatty acids and amino acids, trait analysis (crops and animals)
  • 48. VI. Other platforms (Cont’d) Expanding our research, capacity building and service opportunities 3. Online data integration and analysis platforms iPlant Collaborative (Cyberinfrastructure to support plant biology research. # Sustainable access to high performance computing, interoperable software analysis, and large data sets ibp (Integrated breeding platform: public web-based one-stop shop for information, analytical tools and related services to design and efficiently conduct molecular-assisted breeding experiments)
  • 49. Strategies for new equipment and technologies acquisition
  • 50. Strategies for new equipment and technologies acquisition (Some challenges!!!) BecA Hub Lab asset distribution >100K (30%) 5OK-100K (10%) 10K - 50K (30%) < 10K (30%) Equipment replacement plan # < 100 000 USD (Grants) # > 100 000 USD (special donations) # Capital expenditure ???
  • 51. Thank you Biosciences eastern & central Africa (BecA) Hub International Livestock Research Institute (ILRI), Nairobi, Kenya http://hub.africabiosciences.org
  • 52. The BecA Hub Capacity Building Programme
  • 53. “Biosciences are seen as one of the major engines of growth in the world in fields such as …. agriculture. Africa lags behind in biosciences. The two key problems are lack of sufficient funding from governments and shortage of skilled expertise.” NEPAD www.nepad.org/humancapitaldevelopment/abi
  • 54. Objectives • Strengthen capacity of individuals and institutions to harness the latest biosciences technologies to improve agriculture in Africa • Support African scientists efforts to lead and sustain biosciences research in Africa • Promote access to world-class research and training facilities at the BecA Hub
  • 55. Building biosciences capacity for improving African agriculture 1. Research placements • Graduate students • Visiting scientists 2. Individual/small group training 3. Training workshops 4. Conferences 5. Institutional capacity building 6. Linkages, information, creating awareness of BecA Hub
  • 56. Growing numbers using the Hub Graduate students, visiting scientists and short term trainees
  • 57. Individual/small group training • 1-5 trainees • 1-4 weeks • Customised training • One or more technologies
  • 58. Training workshops • Hosted by the BecA Hub – 2007-2011: 42 training workshops – Examples (2011): • Basic Laboratory Health and Safety (ILRI, BecA) • Marker Assisted Breeding (ICRISAT) • Annual practical training workshops organised by BecA Hub, under ABCF 1. Science paper writing 2. Introduction to molecular biology and bioinformatics 3. Advanced bioinformatics 4. New for 2012: Laboratory management & equipment maintenance
  • 59. Science Writing Workshops: 2009 - 2011 • Applications: 703 (2009), 560 (2011) • 64 participants from 15 African countries (27 women, 37 men) • Outputs (2009, 2010): 24 papers submitted, 15 published • 2009: BMGF funding; training by Scriptoria • 2010, 2011: AusAID funding; training led by CSIRO “I had been struggling for one year with my introduction, but I finished it in one afternoon.” Anne Akol, Senior Lecturer, Makerere University, Uganda
  • 60. Molecular Biology and Bioinformatics Workshop 2011 • 21 participants (6 women, 15 men) • 10 African countries • Research discovery process Tissue DNA PCR Cloning Sequencing BLAST Sequence analysis
  • 61. Advanced bioinformatics workshop 2011: Next Generation Sequencing for Africa • 24 participants (18 men, 6 women) from Africa • 13 tutors from Kenya, Europe, USA • EMBO Global Exchange Lecture Course: Next Generation Sequencing data analysis • 4 collaborations initiated - RVFV (VRI-Sudan, ILRI-BecA) - Finger millet SNPs (KU-Ke, U Liverpool) - Trypanosome MDR (SUA-Tz, U Liverpool) - Drought stress in banana (Makerere U-Ug, BecA)
  • 62. Building capacity through research • Major focus – Post graduate research projects (up to 3-4 yrs) – Students registered at many universities – Visiting scientist placements (up to 6 months) – Employees from NARIs and Universities ABCF: African Biosciences Challenge Fund
  • 63. Making the ABCF possible • Syngenta Foundation for Sustainable Agriculture (SFSA) • BecA-CSIRO partnership funded by the Australian Agency for International Development (AusAID) • The Bill & Melinda Gates Foundation (BMGF) • Ministry for Foreign Affairs, Sweden, through the Swedish International Development Cooperation Agency (SIDA)
  • 64. ABCF Research Fellowships • Large demand for use of BecA Hub: inadequate funds • Enable African scientists access Hub facilities and services, for high quality research addressing African agricultural problems • Researchers from national research institutes and African universities • 3-6 months at the Hub • Competitive basis or targeted ‘fast track’
  • 65. ABCF Research Fellows Charles Masembe Department of Zoology, Makerere University, Uganda Pig diseases and food security: Next-generation DNA sequencing of African swine fever virus (ASFV) in Uganda Selamawit Bedane (Haramaya University, Ethiopia) Sisay Alemu (Holetta Agricultural Research Center, EIAR, Ethiopia) Molecular characterization of enset from Ethiopia using banana microsatellite markers
  • 66. ABCF Research Fellows Dia Hassan Central Veterinary Research Laboratories, Khartoum, Sudan Theileria parva genotyping to support control of East Coast fever, an emerging disease in South Sudan Dora Kilalo Department of Plant Science and Crop Protection, University of Nairobi Passion fruit woodiness disease diagnostics
  • 67. ABCF Research Fellows Alexander Bombom Department of Agricultural Production, Makerere University Molecular characterization of maize-sorghum hybrids Félix Meutchieye University of Dschang, Cameroon Molecular characterization of Cameroon indigenous goats and sheep
  • 68. Building capacity of African institutes • National Agricultural Biotechnology Centre, Holetta, Ethiopia • Gulu University, Uganda • Mikocheni Agricultural Research Institute (MARI), Tanzania
  • 69. Raising awareness 38 institutes and organisations in 8 countries visited in 2011 • Burundi • Cameroon • Ethiopia • Rwanda • Sudan • Tanzania • Uganda • Nigeria • Conferences in many countries • BecA workshops • Website • Printed materials • We host over 1500 visitors per year
  • 70. BecA alumni: Where are they now? IRRI
  • 71. Integration of research, capacity building and research- related services For a better delivery of the BecA mission Research Research Capacity related building services
  • 72. Acknowledgements • Australian Agency for International Development (AusAID)/CSIRO • Bill & Melinda Gates Foundation (BMGF) • Ministry for Foreign Affairs, Sweden, through the Swedish International Development Cooperation Agency (SIDA) • Syngenta Foundation for Sustainable Agriculture (SFSA) • NEPAD/AU • Canadian International Development Agency (CIDA) • Google Foundation • Rockefeller Foundation • Gatsby Charitable Foundation • Doyle Foundation • The Kenyan Government • and many others
  • 73. Thank you IRRI

Notas do Editor

  1. These projects were identified through a series of workshops including African and Australian and other partners, focused on food and nut sec