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Developing and promoting legume based
   cropping systems in Mozambique



              S. Boahen
          Legume Agronomist
           IITA – Mozambique
                Nampula




                                        www.iita.org
Why Legumes?

Importance of grain legumes
- Major source of protein
- Cropping systems – N
  contribution
- Source of income            Cowpea

Growing demand for soybean

Domestic demand estimated
at 45,000 tons/year

Current production ≈ 5,000
tons/yr; yields ≈ 700 kg/ha
Cowpea yields ≈ 300 kg/ha      Soybean
                                         www.iita.org
Major Constraints


Lack of high-yielding adapted varieties
Poor access to seeds and seed delivery system
Limited information on crop management practices
Pests and diseases
Low human and institutional capacity




                                                   www.iita.org
Objectives


Identify and promote high-yielding adapted genotypes
Develop appropriate crop management practices
Facilitate the development of community-based seed
systems
Enhance capacity




                                                       www.iita.org
Research Provinces
- Nampula
- Zambezia
- Manica
- Maputo




                       www.iita.org
Partial aerial view of IITA Research site at Ruace, Zambezia, 2008


                                                              www.iita.org
Soybean screening
                    www.iita.org
Cowpea screening
                   www.iita.org
Variety Selection
                                                                  Nampula - PD 19/01/08                          a
                                                   1600
                                                                                       ab                                     ab

 Potential yield                                   1400

                                                   1200                  ab
                                                                                                    ab
- Genetically determined



                              Seed yield (kg/ha)
                                                   1000
                                                              b


- Management and Env’t                             800

                                                   600

- Critical: Yield stability                        400

                                                   200

                                                     0
                                                          Storm   TGx 1740-2F   TGx 1904-6F   TGx 1485-1D TGx 1910-10F TGx 1908-8F

                                                                                  Soybean genotype




                                                                                                                       www.iita.org
Table 1. Yields of early and medium to late maturing soybean genotypes
 screened for desirable agronomic traits during the 2007/08 growing season.

Genotype          Ruace       Nampula   Genotype        Ruace      Nampula
             Early genotype (kg/ha)       Medium to late genotype (kg/ha)
TGx 1932-1F        2824         2330    TGx 1935-5F      2263        1452
TGx 1935-3F        2700         2217    TGx 1936-2F      1633        1533
TGx 1945-1F        2652         2002    TGx 1937-1F      2691        1672
TGx 1951-3F        2902         2354    TGx 1939-2F      1194        1528
TGx 1951-4F        2498         1957    TGx 1949-7F      2367        1978
TGx 1954-1F        2446         2186    TGx 1949-8F      2043        1767
TGx 1965-7F        2694         1535    TGx 1949-10F     2246        2070
TGx 1971-1F        2635         2404    TGx 1950-8F      2306        2576
TGX 1972-1F        2856         2435    TGx 1955-4F      2765        1517
TGx 1977-2F        2585         2628    TGx 1956-1F      2224        1511
TGx 1977-4F        2743         1769    TGx 1963-3F      3129        1318
 LSD(0.05)          NS           NS       LSD(0.05)        613        917


                                                                      www.iita.org
Table 2. Yields of early and early to medium maturing soybean genotype
       screened for desirable agronomic traits during the 2008/09 growing season.

 Yields of Early genotype (kg/ha)     Yields of early to medium genotype (kg/ha)
TGx 1987-20F              3464       TGx 1986-3F                     2144
TGx 1987-65F              3409       TGx 1985-2F                     2020
TGx 1987-62F              3037       TGx 1984-8F                     1879
TGx 1987-8F               2960       TGx 1985-7F                     1847
TGx 1987-6F               2948       TGx 1985-8F                     1783
TGx 1987-9F               2822       TGx 1985-3F                     1704
TGx 1987-17F              2761       TGx 1985-9F                     1583
TGx 1987-10F              2657       TGx 1986-1F                     1572
TGX 1019-2EN              2610       TGx 1985-4F                     1556
TGx 1987-34F              2570       TGx 1985-10F                    1555
  LSD(0.05)               491           LSD(0.05)                     496



                                                                          www.iita.org
Table 3. Yields of early and medium to late maturing soybean genotype
 screened for desirable agronomic traits during the 2008/09 growing season.

 Yields of Medium genotypes (kg/ha)        Yield of Late genotype (kg/ha)
TGx 1986-38F              3180         TGx 1955-4F                    2575
TGx 1986-14F              3002         TGx 1935-5F                    2541
TGx 1986-19F              2756         TGx 1965-7F                    2523
TGx 1986-40F              2536         TGx 1984-11F                   1812
TGx 1986-37F              2525         TGx 1984-1F                    1770
TGx 1987-3F               2410         TGx 1440-1E                    1760
TGx 1986-15F              2380         TGx 1984-24F                   1706
TGx 1986-35F              2338         TGx 1984-23F                   1504
TGx 1985-12F              1306         TGx 1984-22F                   1496
TGx 1985-2F               1274         TGx 1984-19F                   1477
 LSD(0.05)                 442         LSD(0.05)                       543




                                                                             www.iita.org
Table 4. Top yielding Cowpea lines during 2008 and 2009

                                         Location Yields (kg/ha)
                      Ruace              Nampula                   Sussundenga
Genotype             Year ‘08    Year ‘08       Year ‘09     Year ‘08     Year ‘09
IT 18                  1582        1221          1055          1457        1027
IT 16                  1334        1377          1057          1558         976
INIA-73                1472        1425            865         1484        1236
Sudan -1               1452        1224          1329          1553         830
Brown mixed            1621        1572            896         841          425
IT00K-126-3            1373        1644          1396          1803        1026
IT97K-1069-6           2048        1178          1058          1970        1476
IT98K-205-8            1025        290             589         1068         460
IT97K-499-35           1302        1084            669         1298         721
IT97K-390-2              -           -             764             -       1470
IT98K-131-2              -         876           1133          1201         865
IT98K-412-13             -           -             580             -        750
IT98K-494-6            1081          -              -              -         -
LSD (0.05)             394         261             456         941          518
                                                                            www.iita.org
Table 5. Cowpea lines selected under drought conditions

                                               Yield (kg/ha)     in 2008
 Variety                                    Ruace                Nampula
IT98K-128-3                                 1796                    435
IT98-628                                    1544                    413
IT97K-390-2                                 1997                    473
IT98K166-4                                  1192                    398
IT98K-412-13                                1767                    523
IT98K-491-4                                 1461                    482
IT89KD-288                                  1440                    380
IT97K-819-118                               1461                    481
IT99K-7-21-2-2                              1265                    537
IT99K-529-1                                 1567                    458
IT99K-216-24-2                              1414                    477
IT97K-1069-6                                1924                    531
IT98K-311-8-2                               1470                    252
IT96D-610                                   1261                    445
IT00K-126-3                                 1526                    476
IT99K-112-2                                 1331                    331
LSD(0.05)                                    335                    171
                                                                           www.iita.org
Variety Selection
Diseases and pests
- Resistance and tolerance




                                     www.iita.org
www.iita.org
Crop Management

   When is the best time to plant soybeans?
                                                           4500                                  PD 27/12/07    PD 9/1/08
- Daylength sensitivity                                                                          PD 24/1/08     PD 8/2/08
                                                           4000

- Temperature                                              3500

- Early planting during the season                         3000



                                      Seed yield (kg/ha)
                                                           2500


                                                           2000


                                                           1500


                                                           1000


                                                           500


                                                             0
                                                                   Storm    TGx 1740-2F   TGx 1904-6F   TGx 1485-1D     427/5/7      TGx 1908-8F


I4-day delay: Avg yield ≈ 47 kg/day                                                         Soybean genotypes



47x14MT = 658MT = $24.00/day                                  Effect of planting date on the yields of six of the twelve soybean
                                                              varieties planted in Ruace, Zambezia during the 2007/08 growing
                                                              season.

                                                                                                                                  www.iita.org
5.0
                                                                             PD 11/12/08     PD 24/12/08
                                                                             PD 07/01/09     PD 21/01/09
                                 4.5


                                 4.0


                                 3.5
          Seed yield (tons/ha)


                                 3.0


                                 2.5


                                 2.0


                                 1.5


                                 1.0


                                 0.5


                                 0.0
                                       Storm   TGx 1904-6F TGx 1963-3F TGx 1908-8F TGx 1951-3F TGx 1932-1F TGx 1485-1D
                                                                    Soybean genotypes

Effect of planting date on the yields of seven of the sixteen soybean varieties
planted in Ruace, Zambesia during the 2008/09 growing season.

                                                                                                               www.iita.org
Table 6. Effect of planting date on seed yield variables and their
                contribution seed yield


                                      Seed Yield Variables


Planting     Plant      Days to     Pod-filling     No of        No of   100-seed
Date         stand/m2   Maturity   duration (d)   Pods/plant   seeds/pod   wt (g)

11 Dec ‘08     34.0      105.1         36.3         122.5         2.41         14.7

26 Dec ‘08     32.6       96.5         33.4          98.3         2.37         13.1

10 Jan ‘09     24.4       89.3         31.9         117.3         2.28         11.9

26 Jan ‘09     19.1       83.2         26.9         100.3         2.27         9.7

LSD(0.05)      2.6        0.11         0.45          17.1         0.09         0.15

R2             0.95       0.96         0.94          0.17         0.86         0.98

P-value       0.015      0.013        0.020         0.529        0.047        0.006


                                                                             www.iita.org
When is the best time to plant?


                                    Ruace, Zambesia                                                        Nampula
                     2500                                                            2500
                                A                    PD 26/1/08                                  B                              PD 2/1/08
                                                     PD 9/2/08                                                                  PD 22/1/08
                                                     PD 4/3/08                                                                  PD 18/2/08
                     2000                                                            2000
                                                     PD 15/3/08
Seed yield (kg/ha)




                     1500                                                            1500



                     1000                                                            1000



                      500                                                             500



                        0                                                               0
                            IT00K1263   Sudan-1     Brown mixed IT97K10696   IT-18          IT00K-1263   INIA-73        IT-16               IT-18   IT97K-499-35

                                                  Cowpea Varieties                                                 Cowpea Varieties



                        Effect of planting date on the yields of five of the ten cowpea varieties planted in Ruace (A) and Nampula
                        (B) during the 2008 growing season.




                                                                                                                                                         www.iita.org
Biological nitrogen fixation


 High nitrogen-fixing ability
- Promiscuous nodulators
- Inoculation methods




     Liquid        Peat-based    Granular


                                                          www.iita.org
a
                                                                 50                                            Check               Liquid A        Peat A
                                                                                  a                            Ganular A           Liquid B        Peat B
                                                                 45

                                                                 40
                                                                                                                                               a




                                       Number of nodules/plant
                                                                 35

 Rhizobium                                                       30
                                                                                       b


                                                                 25
 inoculation                                                     20       c
                                                                                                  c
                                                                                                        cb                     b
                                                                                                                                      b                 b
                                                                 15                                                                                cb
                                                                 10
                                                                                                                       c
                                                                  5
Nodule numbers from various
                                                                  0
inoculation treatments average                                                        Ruace                                        Nampula
over two soybean varieties.                                                                       Experimental Sites


                                                                 3000


                                                                 2900                                                      a

                                                                                           ab
                                                                 2800
                                                                                                          ab
                                       Yield (kg/ha)




                                                                 2700
                                                                                                                                                        ab
                                                                                                                                          ab
                                                                 2600         b


                                                                 2500

Grain yield from various inoculation                             2400
treatments average over two soybean
varieties at Ruace.                                              2300
                                                                        Check         Liquid A        Peat A    Ganular A           Liquid B        Peat B
                                                                                                  Inoculation Treatment


                                                                                                                                                             www.iita.org
Response to Phosphorous
TGx 1740-2F selected for low P
tolerance
Optimum - 20kg P/ha
                                        6000                                                       2000
                                               A                                                          B
                                                                                                   1800
                                        5000
                                                                                                   1600

                                                                                                   1400
                                        4000
                   Seed yield (kg/ha)




                                                                                                   1200

                                        3000                                                       1000

                                                                                                   800
                                                               427/5/7
                                        2000                                                                          Soybean
                                                               Ocepara                             600
                                                                                                                      Cow pea
                                                               Solitaire
                                                                                                   400
                                        1000                   TGx 1740-2F

                                                                                                   200

                                          0                                                          0
                                               0 kg P/ha   20 kg P/ha    40 kg P/ha   60 kg P/ha          0 kg P/ha   20 kg P/ha    40 kg P/ha     60 kg P/ha
                                                           Phosphorous Application                                    Phosphorous Application


      Soybean yields in Ruace in response to phosphorus application (A); and yields of cowpea and soybean
      averaged over four varieties of each crop in response to P application at Nampula (B).

                                                                                                                                                 www.iita.org
Selected genotypes

Genotypes selected based on screening and evaluation:

-Soybean: TGx 1740-2F, TGx 1908-8F, TGx 1937-1F, TGx
1904-6F and TGx 1485-1D

-Cowpea: IT98K-128-3, IT97K-390-2, IT97K-1069-6,
IT98K-131-2, IT00K-126-3, IT-16 and IT-18




                                                   www.iita.org
Seed Systems - Approaches
Seed Multiplication
National Performance Trials
On-farm demonstration in collaboration with partners
Linkage with farmer organizations for community-based
seed production
Worked with IIAM on Varietal release




                                                   www.iita.org
Off-season seed multiplication
- For on-farm demonstrations with partners
- To Facilitate community-based seed systems development


                                                           www.iita.org
www.iita.org
www.iita.org
Table 7. Yields of selected soybean genotypes grown
                in various agro-ecologies for NPT in 2008/09 growing
                season.
                                Location Yields (kg/ha)
Genotype
                 Ruace    Nampula Sussundenga        Namialo    Umbeluzi
TGx 1740-2F       3195      1372         1314          2442       2354
TGx 1908-8F       4369      1576         1718          1720       1344
TGx 1904-6F       4716      1947         1809          2280       1847
TGx 1937-1F       3693      1487         1214          1598        583
TGx 1485-1D       4153      1519         1236          1720       2175
Storm (Check)     3749      1130         1340          1042       1414
CV                 8.8        48           35           39         21
LSD(0.05)          530       NS           NS           1070        520


                                                                   www.iita.org
Table 8. Days to flowering and maturity of selected
        soybean genotypes at Ruace and Umbeluzi

Genotype                     Ruace                     Umbeluzi
                  Days to       Days to       Days to         Days to
                 Flowering      Maturity     Flowering        Maturity
TGx 1740-2F         40.0          86.5          49.7           134.0
TGx 1908-8F         47.0          98.0          66.0           138.5
TGx 1904-6F         49.2          97.7          63.0           125.7
TGx 1937-1F         57.2         100.5          73.0           139.5
TGx 1485-1D         44.0          86.7          65.2           133.2
Storm (Check)       36.7          80.5          49.2           116.7
CV                  1.36          1.18          1.49           0.91
LSD(0.05)           0.94          1.64          1.37           1.80


                                                               www.iita.org
Maturity duration
- Select variety to fit the growing
  season
- TGx 1937-1F doesn’t fit lat >17o S        S15o




                                            S19o




                Daylength increases         S26o


                                       www.iita.org
Table 9. Yield of selected cowpea genotypes in three agro-
        ecologies.
                            Location yields (kg/ha) and planting dates
                       Ruace                 Nampula           Sussundenga

Genotype       Feb 27 Mar        4, Jan 2, ‘08 Jan      14 Jan 31,’08 Jan        13
               ‘08    ‘08                      ‘09                    ‘09
IT00K-126-3     1527      1373        1644       1394        2087        1026
IT97K-1069-6    1924      2048        1178       1058        1580        1476
IT98K-128-3     1796        -           -         644          -          460
IT97K-390-2     1997        -           -         764          -         1470
IT98K-131-2      -        1051         876       1133        1083         865
IT-16            -        1334        1377       1045         748         976
IT-18            -        1582        1221       1048        1527        1027
CV              13.1      19.5        15.1       34.9         90          38.3
LSD             334       393          261        456         NS          518

                                                                         www.iita.org
On-farm demonstrations – collaboration with CLUSA and IKURU
-Cowpea: 355 Farmers and 1065 demonstrations plots in 25
communities
- Soybean: 68 Farmers and 204 demonstration plots in 8
communities

                                                              www.iita.org
www.iita.org
www.iita.org
Table 10. Soybean yields from farmer managed trials across several
                       communities in Zambesia Province in the 2008/09 growing season.

Genotype                                                Location Yields (kg/ha)
                                   Ruace             Tetete           Magige               Lioma    Serra
TGx 1740-2F                        3348a             2100a             3117a               3013a    2431a
TGx 1908-8F                        3129bc           1956ab             2883a               2904a    2171b
TGx 1904-6F                        3002c            1839a              2925a               2652a    2065b
TGx 1937-1F                        3083c            1904ab             2967a               2584a    2095b
TGx 1485-1D                        3220ab            2075a             3033a               2719a    2212ab
              Storm                2397d                 -                 -               2585a    1610c
Local check




              Santa Rosa           1770e             1217c             1750b               1625b    1255d
              Safari               1567e             1049c              251c                   -    1810bc
              Santa                1771e             576d                  -               1734b       -
 LSMEANS within a column followed by the same letter are not significantly different at P ˂ 0.05.


                                                                                                     www.iita.org
Table 11. Yields of improved and local cowpea lines from
                          farmer-managed trials in 2009


Genotype                                                   Location Yield (kg/ha)†

                    Rapale          Mogovol        Angoche          Angoche -        Rapale          Monapo
                    Marranta        as             Namapon          Serracao         Anchilo-        Neita-
                    ne              Muatua         da                                Namigonha       Muapala

IT-18                   461c           400c            502a             483a                548a       653b

IT00K-126-3            631a            550b            556a             478a                470b       832a

IT97K-1069-6           554b            925a            569a             568a                400c       534c

Namurua                240d            365c            173b             192b                248d       127d
(Check)

 †LSMEANS within a column followed by the same letter are not significantly different at P ˂ 0.05.


                                                                                                       www.iita.org
Seeds of selected cowpea sent to Zimbabwe,
Zambia and Tanzania for testing              www.iita.org
Main season seed multiplication
Harvested 12.5 tons


                                  www.iita.org
Main season seed multiplication
- Harvested 5.6 tons

                                  www.iita.org
On-going off-season seed multiplication
of soybean rust tolerant lines
TGx 1987-20F; TGx 1987-65F;
TGx 1987-62F; TGx 1987-8F;
TGx 1987-9F; TGx 1986-38F
TGx 1987-14F
                                          www.iita.org
www.iita.org
www.iita.org
www.iita.org
Seed Systems - Summary
12.5 tons Soybean and 5.6 tons cowpea seeds produced to
facilitate community seed production scheme

Data from NPT available

Data from on-farm demonstrations available

Conducted field days, TV program to promote technologies

5 soybean and 5 cowpea genotypes submitted for
registration and release


                                                    www.iita.org
Capacity building

  Human capacity
 – 2500 farmers and extension agents trained
 - 1I non degree and 6 degree students from IITA and IIAM
 Institutional capacity
 - Water pump for off-season seed multiplication
 - Physiological equipments and other basic lab equipments
 - Tractor and farm implements




                                                      www.iita.org
Future plans
Continue testing and identification of high-yielding adapted soybean
and cowpea for registration and release to provide more options to
producers
 Asia soybean rust is a major priority for the SA region. I will collaborate
with the soybean breeder and pathologist to identify resistance varieties
Study the leaf gas exchange and water use efficiency of genotypes
selected for drought tolerance
Scale-up seed multiplication to facilitate the development of a
sustainable community-based seed system
Develop efficient inoculants application strategies through assessing
the need for rhizobial inoculant use; characterization of indigenous
bradyrhizobial populations, testing native and introduced strains for
host-strain compatibility and competitiveness
Quantify N and non-N benefits to crops following soybean and cowpea
in rotation.

                                                                    www.iita.org
Acknowledgements


USAID

Tropical Legume II Project

Research Technicians




                                www.iita.org
www.iita.org
THANK YOU




            www.iita.org

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Developing and promoting legume based cropping systems in Mozambique

  • 1. Developing and promoting legume based cropping systems in Mozambique S. Boahen Legume Agronomist IITA – Mozambique Nampula www.iita.org
  • 2. Why Legumes? Importance of grain legumes - Major source of protein - Cropping systems – N contribution - Source of income Cowpea Growing demand for soybean Domestic demand estimated at 45,000 tons/year Current production ≈ 5,000 tons/yr; yields ≈ 700 kg/ha Cowpea yields ≈ 300 kg/ha Soybean www.iita.org
  • 3. Major Constraints Lack of high-yielding adapted varieties Poor access to seeds and seed delivery system Limited information on crop management practices Pests and diseases Low human and institutional capacity www.iita.org
  • 4. Objectives Identify and promote high-yielding adapted genotypes Develop appropriate crop management practices Facilitate the development of community-based seed systems Enhance capacity www.iita.org
  • 5. Research Provinces - Nampula - Zambezia - Manica - Maputo www.iita.org
  • 6. Partial aerial view of IITA Research site at Ruace, Zambezia, 2008 www.iita.org
  • 7. Soybean screening www.iita.org
  • 8. Cowpea screening www.iita.org
  • 9. Variety Selection Nampula - PD 19/01/08 a 1600 ab ab Potential yield 1400 1200 ab ab - Genetically determined Seed yield (kg/ha) 1000 b - Management and Env’t 800 600 - Critical: Yield stability 400 200 0 Storm TGx 1740-2F TGx 1904-6F TGx 1485-1D TGx 1910-10F TGx 1908-8F Soybean genotype www.iita.org
  • 10. Table 1. Yields of early and medium to late maturing soybean genotypes screened for desirable agronomic traits during the 2007/08 growing season. Genotype Ruace Nampula Genotype Ruace Nampula Early genotype (kg/ha) Medium to late genotype (kg/ha) TGx 1932-1F 2824 2330 TGx 1935-5F 2263 1452 TGx 1935-3F 2700 2217 TGx 1936-2F 1633 1533 TGx 1945-1F 2652 2002 TGx 1937-1F 2691 1672 TGx 1951-3F 2902 2354 TGx 1939-2F 1194 1528 TGx 1951-4F 2498 1957 TGx 1949-7F 2367 1978 TGx 1954-1F 2446 2186 TGx 1949-8F 2043 1767 TGx 1965-7F 2694 1535 TGx 1949-10F 2246 2070 TGx 1971-1F 2635 2404 TGx 1950-8F 2306 2576 TGX 1972-1F 2856 2435 TGx 1955-4F 2765 1517 TGx 1977-2F 2585 2628 TGx 1956-1F 2224 1511 TGx 1977-4F 2743 1769 TGx 1963-3F 3129 1318 LSD(0.05) NS NS LSD(0.05) 613 917 www.iita.org
  • 11. Table 2. Yields of early and early to medium maturing soybean genotype screened for desirable agronomic traits during the 2008/09 growing season. Yields of Early genotype (kg/ha) Yields of early to medium genotype (kg/ha) TGx 1987-20F 3464 TGx 1986-3F 2144 TGx 1987-65F 3409 TGx 1985-2F 2020 TGx 1987-62F 3037 TGx 1984-8F 1879 TGx 1987-8F 2960 TGx 1985-7F 1847 TGx 1987-6F 2948 TGx 1985-8F 1783 TGx 1987-9F 2822 TGx 1985-3F 1704 TGx 1987-17F 2761 TGx 1985-9F 1583 TGx 1987-10F 2657 TGx 1986-1F 1572 TGX 1019-2EN 2610 TGx 1985-4F 1556 TGx 1987-34F 2570 TGx 1985-10F 1555 LSD(0.05) 491 LSD(0.05) 496 www.iita.org
  • 12. Table 3. Yields of early and medium to late maturing soybean genotype screened for desirable agronomic traits during the 2008/09 growing season. Yields of Medium genotypes (kg/ha) Yield of Late genotype (kg/ha) TGx 1986-38F 3180 TGx 1955-4F 2575 TGx 1986-14F 3002 TGx 1935-5F 2541 TGx 1986-19F 2756 TGx 1965-7F 2523 TGx 1986-40F 2536 TGx 1984-11F 1812 TGx 1986-37F 2525 TGx 1984-1F 1770 TGx 1987-3F 2410 TGx 1440-1E 1760 TGx 1986-15F 2380 TGx 1984-24F 1706 TGx 1986-35F 2338 TGx 1984-23F 1504 TGx 1985-12F 1306 TGx 1984-22F 1496 TGx 1985-2F 1274 TGx 1984-19F 1477 LSD(0.05) 442 LSD(0.05) 543 www.iita.org
  • 13. Table 4. Top yielding Cowpea lines during 2008 and 2009 Location Yields (kg/ha) Ruace Nampula Sussundenga Genotype Year ‘08 Year ‘08 Year ‘09 Year ‘08 Year ‘09 IT 18 1582 1221 1055 1457 1027 IT 16 1334 1377 1057 1558 976 INIA-73 1472 1425 865 1484 1236 Sudan -1 1452 1224 1329 1553 830 Brown mixed 1621 1572 896 841 425 IT00K-126-3 1373 1644 1396 1803 1026 IT97K-1069-6 2048 1178 1058 1970 1476 IT98K-205-8 1025 290 589 1068 460 IT97K-499-35 1302 1084 669 1298 721 IT97K-390-2 - - 764 - 1470 IT98K-131-2 - 876 1133 1201 865 IT98K-412-13 - - 580 - 750 IT98K-494-6 1081 - - - - LSD (0.05) 394 261 456 941 518 www.iita.org
  • 14. Table 5. Cowpea lines selected under drought conditions Yield (kg/ha) in 2008 Variety Ruace Nampula IT98K-128-3 1796 435 IT98-628 1544 413 IT97K-390-2 1997 473 IT98K166-4 1192 398 IT98K-412-13 1767 523 IT98K-491-4 1461 482 IT89KD-288 1440 380 IT97K-819-118 1461 481 IT99K-7-21-2-2 1265 537 IT99K-529-1 1567 458 IT99K-216-24-2 1414 477 IT97K-1069-6 1924 531 IT98K-311-8-2 1470 252 IT96D-610 1261 445 IT00K-126-3 1526 476 IT99K-112-2 1331 331 LSD(0.05) 335 171 www.iita.org
  • 15. Variety Selection Diseases and pests - Resistance and tolerance www.iita.org
  • 17. Crop Management When is the best time to plant soybeans? 4500 PD 27/12/07 PD 9/1/08 - Daylength sensitivity PD 24/1/08 PD 8/2/08 4000 - Temperature 3500 - Early planting during the season 3000 Seed yield (kg/ha) 2500 2000 1500 1000 500 0 Storm TGx 1740-2F TGx 1904-6F TGx 1485-1D 427/5/7 TGx 1908-8F I4-day delay: Avg yield ≈ 47 kg/day Soybean genotypes 47x14MT = 658MT = $24.00/day Effect of planting date on the yields of six of the twelve soybean varieties planted in Ruace, Zambezia during the 2007/08 growing season. www.iita.org
  • 18. 5.0 PD 11/12/08 PD 24/12/08 PD 07/01/09 PD 21/01/09 4.5 4.0 3.5 Seed yield (tons/ha) 3.0 2.5 2.0 1.5 1.0 0.5 0.0 Storm TGx 1904-6F TGx 1963-3F TGx 1908-8F TGx 1951-3F TGx 1932-1F TGx 1485-1D Soybean genotypes Effect of planting date on the yields of seven of the sixteen soybean varieties planted in Ruace, Zambesia during the 2008/09 growing season. www.iita.org
  • 19. Table 6. Effect of planting date on seed yield variables and their contribution seed yield Seed Yield Variables Planting Plant Days to Pod-filling No of No of 100-seed Date stand/m2 Maturity duration (d) Pods/plant seeds/pod wt (g) 11 Dec ‘08 34.0 105.1 36.3 122.5 2.41 14.7 26 Dec ‘08 32.6 96.5 33.4 98.3 2.37 13.1 10 Jan ‘09 24.4 89.3 31.9 117.3 2.28 11.9 26 Jan ‘09 19.1 83.2 26.9 100.3 2.27 9.7 LSD(0.05) 2.6 0.11 0.45 17.1 0.09 0.15 R2 0.95 0.96 0.94 0.17 0.86 0.98 P-value 0.015 0.013 0.020 0.529 0.047 0.006 www.iita.org
  • 20. When is the best time to plant? Ruace, Zambesia Nampula 2500 2500 A PD 26/1/08 B PD 2/1/08 PD 9/2/08 PD 22/1/08 PD 4/3/08 PD 18/2/08 2000 2000 PD 15/3/08 Seed yield (kg/ha) 1500 1500 1000 1000 500 500 0 0 IT00K1263 Sudan-1 Brown mixed IT97K10696 IT-18 IT00K-1263 INIA-73 IT-16 IT-18 IT97K-499-35 Cowpea Varieties Cowpea Varieties Effect of planting date on the yields of five of the ten cowpea varieties planted in Ruace (A) and Nampula (B) during the 2008 growing season. www.iita.org
  • 21. Biological nitrogen fixation High nitrogen-fixing ability - Promiscuous nodulators - Inoculation methods Liquid Peat-based Granular www.iita.org
  • 22. a 50 Check Liquid A Peat A a Ganular A Liquid B Peat B 45 40 a Number of nodules/plant 35 Rhizobium 30 b 25 inoculation 20 c c cb b b b 15 cb 10 c 5 Nodule numbers from various 0 inoculation treatments average Ruace Nampula over two soybean varieties. Experimental Sites 3000 2900 a ab 2800 ab Yield (kg/ha) 2700 ab ab 2600 b 2500 Grain yield from various inoculation 2400 treatments average over two soybean varieties at Ruace. 2300 Check Liquid A Peat A Ganular A Liquid B Peat B Inoculation Treatment www.iita.org
  • 23. Response to Phosphorous TGx 1740-2F selected for low P tolerance Optimum - 20kg P/ha 6000 2000 A B 1800 5000 1600 1400 4000 Seed yield (kg/ha) 1200 3000 1000 800 427/5/7 2000 Soybean Ocepara 600 Cow pea Solitaire 400 1000 TGx 1740-2F 200 0 0 0 kg P/ha 20 kg P/ha 40 kg P/ha 60 kg P/ha 0 kg P/ha 20 kg P/ha 40 kg P/ha 60 kg P/ha Phosphorous Application Phosphorous Application Soybean yields in Ruace in response to phosphorus application (A); and yields of cowpea and soybean averaged over four varieties of each crop in response to P application at Nampula (B). www.iita.org
  • 24. Selected genotypes Genotypes selected based on screening and evaluation: -Soybean: TGx 1740-2F, TGx 1908-8F, TGx 1937-1F, TGx 1904-6F and TGx 1485-1D -Cowpea: IT98K-128-3, IT97K-390-2, IT97K-1069-6, IT98K-131-2, IT00K-126-3, IT-16 and IT-18 www.iita.org
  • 25. Seed Systems - Approaches Seed Multiplication National Performance Trials On-farm demonstration in collaboration with partners Linkage with farmer organizations for community-based seed production Worked with IIAM on Varietal release www.iita.org
  • 26. Off-season seed multiplication - For on-farm demonstrations with partners - To Facilitate community-based seed systems development www.iita.org
  • 29. Table 7. Yields of selected soybean genotypes grown in various agro-ecologies for NPT in 2008/09 growing season. Location Yields (kg/ha) Genotype Ruace Nampula Sussundenga Namialo Umbeluzi TGx 1740-2F 3195 1372 1314 2442 2354 TGx 1908-8F 4369 1576 1718 1720 1344 TGx 1904-6F 4716 1947 1809 2280 1847 TGx 1937-1F 3693 1487 1214 1598 583 TGx 1485-1D 4153 1519 1236 1720 2175 Storm (Check) 3749 1130 1340 1042 1414 CV 8.8 48 35 39 21 LSD(0.05) 530 NS NS 1070 520 www.iita.org
  • 30. Table 8. Days to flowering and maturity of selected soybean genotypes at Ruace and Umbeluzi Genotype Ruace Umbeluzi Days to Days to Days to Days to Flowering Maturity Flowering Maturity TGx 1740-2F 40.0 86.5 49.7 134.0 TGx 1908-8F 47.0 98.0 66.0 138.5 TGx 1904-6F 49.2 97.7 63.0 125.7 TGx 1937-1F 57.2 100.5 73.0 139.5 TGx 1485-1D 44.0 86.7 65.2 133.2 Storm (Check) 36.7 80.5 49.2 116.7 CV 1.36 1.18 1.49 0.91 LSD(0.05) 0.94 1.64 1.37 1.80 www.iita.org
  • 31. Maturity duration - Select variety to fit the growing season - TGx 1937-1F doesn’t fit lat >17o S S15o S19o Daylength increases S26o www.iita.org
  • 32. Table 9. Yield of selected cowpea genotypes in three agro- ecologies. Location yields (kg/ha) and planting dates Ruace Nampula Sussundenga Genotype Feb 27 Mar 4, Jan 2, ‘08 Jan 14 Jan 31,’08 Jan 13 ‘08 ‘08 ‘09 ‘09 IT00K-126-3 1527 1373 1644 1394 2087 1026 IT97K-1069-6 1924 2048 1178 1058 1580 1476 IT98K-128-3 1796 - - 644 - 460 IT97K-390-2 1997 - - 764 - 1470 IT98K-131-2 - 1051 876 1133 1083 865 IT-16 - 1334 1377 1045 748 976 IT-18 - 1582 1221 1048 1527 1027 CV 13.1 19.5 15.1 34.9 90 38.3 LSD 334 393 261 456 NS 518 www.iita.org
  • 33. On-farm demonstrations – collaboration with CLUSA and IKURU -Cowpea: 355 Farmers and 1065 demonstrations plots in 25 communities - Soybean: 68 Farmers and 204 demonstration plots in 8 communities www.iita.org
  • 36. Table 10. Soybean yields from farmer managed trials across several communities in Zambesia Province in the 2008/09 growing season. Genotype Location Yields (kg/ha) Ruace Tetete Magige Lioma Serra TGx 1740-2F 3348a 2100a 3117a 3013a 2431a TGx 1908-8F 3129bc 1956ab 2883a 2904a 2171b TGx 1904-6F 3002c 1839a 2925a 2652a 2065b TGx 1937-1F 3083c 1904ab 2967a 2584a 2095b TGx 1485-1D 3220ab 2075a 3033a 2719a 2212ab Storm 2397d - - 2585a 1610c Local check Santa Rosa 1770e 1217c 1750b 1625b 1255d Safari 1567e 1049c 251c - 1810bc Santa 1771e 576d - 1734b - LSMEANS within a column followed by the same letter are not significantly different at P ˂ 0.05. www.iita.org
  • 37. Table 11. Yields of improved and local cowpea lines from farmer-managed trials in 2009 Genotype Location Yield (kg/ha)† Rapale Mogovol Angoche Angoche - Rapale Monapo Marranta as Namapon Serracao Anchilo- Neita- ne Muatua da Namigonha Muapala IT-18 461c 400c 502a 483a 548a 653b IT00K-126-3 631a 550b 556a 478a 470b 832a IT97K-1069-6 554b 925a 569a 568a 400c 534c Namurua 240d 365c 173b 192b 248d 127d (Check) †LSMEANS within a column followed by the same letter are not significantly different at P ˂ 0.05. www.iita.org
  • 38. Seeds of selected cowpea sent to Zimbabwe, Zambia and Tanzania for testing www.iita.org
  • 39. Main season seed multiplication Harvested 12.5 tons www.iita.org
  • 40. Main season seed multiplication - Harvested 5.6 tons www.iita.org
  • 41. On-going off-season seed multiplication of soybean rust tolerant lines TGx 1987-20F; TGx 1987-65F; TGx 1987-62F; TGx 1987-8F; TGx 1987-9F; TGx 1986-38F TGx 1987-14F www.iita.org
  • 45. Seed Systems - Summary 12.5 tons Soybean and 5.6 tons cowpea seeds produced to facilitate community seed production scheme Data from NPT available Data from on-farm demonstrations available Conducted field days, TV program to promote technologies 5 soybean and 5 cowpea genotypes submitted for registration and release www.iita.org
  • 46. Capacity building  Human capacity – 2500 farmers and extension agents trained - 1I non degree and 6 degree students from IITA and IIAM  Institutional capacity - Water pump for off-season seed multiplication - Physiological equipments and other basic lab equipments - Tractor and farm implements www.iita.org
  • 47. Future plans Continue testing and identification of high-yielding adapted soybean and cowpea for registration and release to provide more options to producers Asia soybean rust is a major priority for the SA region. I will collaborate with the soybean breeder and pathologist to identify resistance varieties Study the leaf gas exchange and water use efficiency of genotypes selected for drought tolerance Scale-up seed multiplication to facilitate the development of a sustainable community-based seed system Develop efficient inoculants application strategies through assessing the need for rhizobial inoculant use; characterization of indigenous bradyrhizobial populations, testing native and introduced strains for host-strain compatibility and competitiveness Quantify N and non-N benefits to crops following soybean and cowpea in rotation. www.iita.org
  • 48. Acknowledgements USAID Tropical Legume II Project Research Technicians www.iita.org
  • 50. THANK YOU www.iita.org