1. GREESHMA MOHAN
790 NW 185th Ave Phone: (813) 451-5049
Beaverton, OR 97006 Email: mohan.greeshm@gmail.com
EDUCATION CREDENTIALS
Ph. D. Biomedical Engineering (GPA of 4/4) completion - 05/2015
University of South Florida - Tampa, FL
Master of Science in Bioengineering (GPA of 3.78/4) 08/2010
Clemson University - Clemson, SC
Bachelor of Engineering in Biotechnology (GPA of 8.81/10) 05/2007
Vellore Institute of Technology - India
Results driven dynamic individual with diverse and interdisciplinary work experience including material fabrication, polymer surface chemistry modification, characterization, validation, metrology and statistical analyses of large volumes of data, cell culture techniques, fluorescent microscopy, protein assays. Sustains and owns three laboratory modules - Nikon Eclipse Ti-U Microscope, Plasma Etch PE-50 and BioTek Synergy microplate-reader. Has innate attention to detail, assimilation of feedback for defect identification and in depth troubleshooting skills to attain results with excellent reproducibility, quality and yield over process variables used such as materials, methods, recipes and tools.
AREAS OF EXPERTISE
Process flow design & Optimization
Equipment ownership & sustaining
Metrology & Automation
Project Management
Characterization & Validation
Problem Solving
Research & Stat Data Analysis
Team Building & Mentoring
SPC & Design of Experiments
KEY SKILLS ASSESSMENT
ENGINEERING AND CELL BIOLOGY
Proficient in process specifications for development of biomaterial components, polymer surface chemistry modification, material characterization and cell culture techniques.
Expert in design and fabrication of a soft combinatorial biomaterial substrate with mechanical stiffness and surface chemistry gradients for high throughput screening of cell-substrate interactions.
Gained in depth knowledge of complex engineering concepts and cell biology including manufacturing improvement and sustaining product flow, filtration & recovery, characterization, metrology and testing.
Cell culture of Human Umbilical Vein Endothelial Cells, Primary Aortic Smooth Muscle Cells and NIH3T3 cell line, fluorescent labeling of protein and cells, ELISA assay.
RESEARCH AND DATA ANALYSIS
Skilled in the collecting and analyzing research data utilizing complex biomedical tools/equipment, and procedures such as cGDP and cGLP. Well versed in analyzing and optimizing unit operations, fabrication and modification of polymeric surfaces.
Programmed macros with NIS Elements software on a Nikon Eclipse Ti-U Microscope equipment for running advanced high throughput automated fluorescent imaging & data acquisition.
Trained and skilled in other tools such as spectrophotometric techniques such as BioTek Synergy microplate reader, XPS, atomic force microscopy, tensile testing, column chromatography, oxygen plasma cleaner.
Proficient in data collection, analysis, control charts and interpretation using statistical analysis software. Background in SigmaPlot, SAS, Windows Chart 5 & LabVIEW.
PROJECT MANAGEMENT
Well-versed in planning, organizing and implementing R&D projects while working in teams and/or independently.
Prioritizes tasks for continuous process improvements for strategic direction of product yield & profitability.
Embraces members of diverse cultures and backgrounds in the team set up for enhanced productivity.
Prudent engineering judgment and decision making skills.
2. Greeshma Mohan Resume, Page 2
PROFESSIONAL EXPERIENCE
University of South Florida, Tampa, FL
RESEARCH ASSISTANT - MECHANICAL ENGINEERING 05/2011 – Present
Expert in the fabrication of a combinatorial biomaterial substrate with multidirectional gradients.
Culture and Fluorescent staining of Human Umbilical Vein Endothelial Cells and NIH3T3 cell line on fabricated material for high throughput data extraction and statistical analysis of cell-substrate interactions to validate design parameters for implantable medical devices.
Collaborated with engineering to customize novel hardware architecture of experiment setup and optimization of process control parameters. Used cell & molecular biology methods and tools to study cell adhesive interactions.
RESEARCH MENTOR - MECHANICAL ENGINEERING 05/2011 – 08/2013
Motivated young scientists to pursue engineering career by demonstrating diverse complex engineering concepts through laboratory experiments.
TEACHING ASSISTANT - CHEMICAL & BIOMEDICAL ENGINEERING 08/2010 – 05/2012
Developed and facilitated coursework while providing guidance to students on homework and mini-projects.
Clemson University, Clemson, SC 08/2008 – 08/2010
RESEARCH ASSISTANT – BIOENGINEERING
Cell culture of Primary Aortic Smooth Muscle Cells and conducted various molecular biology assays such as Western Blotting, Immuno-histochemistry, BCA Protein Assay and Zymography for detection of enzyme activity.
Sultan Qaboos University, Oman 10/2007 – 04/2008
RESEARCH ASSISTANT TRAINEE
Performed statistical data analysis and conducted extraction of arteries & protein to study the effect of chronic Lisinopril administration on heart rate variability in spontaneously hypertensive rats.
IICT, Council of Scientific & Industrial Research, India 01/2006 – 04/2007
PROJECT TRAINEE
Instrumental in the enzymatic conversion of agricultural waste to a commercially valuable product xylitol.
INTERNSHIP EXPERIENCE
ZAIN, OMAN NATIONAL DAIRY PRODUCTS, OMAN 05/2005
PROCESS HACCP, QA/QC INTERN
MC DOWELLS INDIA LIMITED, INDIA 12/2005
BREWERY WASTE-MANAGEMENT ENGINEERING INTERN
SUVEN LIFE SCIENCES LTD, INDIA 05/2006
PHARMACEUTICAL DOWNSTREAM PROCESS ENGINEERING INTERN
SELECTED JOURNAL PUBLICATIONS & CONFERENCE PRESENTATIONS
Mohan, Greeshma and Gallant, Nathan, D. Surface chemistry gradients on silicone elastomers for high-throughput modulation of cell-adhesive interfaces, Journal of Biomedical Materials Research: Part A. Published 18th Oct 2014.
Mohan, Greeshma and Gallant, Nathan, D. Fabrication of a combinatorial biomaterial with multi-directional gradients to screen cell mechanobiology. In preparation.
Mohan, Greeshma and Gallant, Nathan, D., Combinatorial Screening of Cell Response to Surface Chemistry Gradient on a Soft Biomaterial, Society For Biomaterials, Boston, MA, April 2013.
Mohan, Greeshma and Gallant, Nathan, D., Cell Response to Surface Chemistry Gradient on a Soft Biomaterial: A Combinatorial Approach, NanoBio Collaborative International Conference, Tampa, FL, March 2012.
Mohan, Greeshma, Cooper, Joel; Novak, Anthony; Gallant, Nathan Fabrication of Combinatorial Biomaterials to Investigate Cell Response, ResearchDay, Tampa, FL, 2011.
S. S. Al-Siyabi, H. Ba-Omar, Greeshma Mohan and S. A Albarwani, The effect of chronic Lisinopril administration on heart rate variability in spontaneously hypertensive rats, Journal of the Federation of American Societies of Experimental Biology, Ib149, 2008.