1. Metagenomics
MICROBIOLOGY LABORATORY
SUBMITTED TO
DR. WALEED ALBASHA
PREPARED BY:
GHAYDA’A SHAQOUR
2012-2013

2. Introduction
 Microbes play an enormous role
in processes that relate to nearly
every aspect of our planet
 Microbes Maintain the
atmosphere
 Microbes Keep Us Healthy
 Microbes Clean Up Fuel Leaks

3. Metagenomics
 It is both a set of research
techniques
 Meta means “transcendent”
 “Metagenomics” refers to the
collection of genomes of complex
microbial communities, and
these communities include many
different types of microbes

4. Metagenomics
 Millions of organisms in one sample
 Millions of random fragments of DNA
 It is like jigsaw puzzles

5. Procedures
 Environmental Shotgun
Sequencing (ESS):
A. Sampling from habitat
B. Filtering particles,
typically by size
C. Lysis and DNA extraction
D. Cloning and library
construction
E. Sequencing the clones
F. Sequence assembly into
contigs and scaffolds

6. Procedures
 High-throughput
sequencing:
1. Illumina Genome
Analyzer II
2. Applied Biosystems
SOLiD system

7. Illumina Genome Analyzer II

8. MetaHIT
 Human intestinal microbial
metagenome
 Understand the role of microbes
in health and in GI disorders
such as IBS, obesity and
metabolic syndrome.

9. Potential contributions
 Earth Sciences
 Life Sciences
 Biomedical Sciences
 Bioenergy
 Biotechnology
 Biodefense and Microbial Forensics

10. Read more
 The New Science of Metagenomics: Revealing the
Secrets of Our Microbial Planet
free PDF downloade
http://www.nap.edu/catalog/11902.html
 Metagenomics of the Human Intestinal Tract
http://www.metahit.eu

11. References
 http://www.cloud-
sequencing.com/service/service_76.aspx?typeid=4
 http://www.ucc.ie/research/apc/content/research/bacte
rial_genomics.html
 http://mgrc.com.my/new/services-metagenomics.html
 http://www.interacademies.net/cms/7665.aspx
 http://www.horizonpress.com/metagenomics
 http://en.wikipedia.org/wiki/Metagenomics
 http://www.metahit.eu/index.php?id=410
 http://www.mayomedicallaboratories.com/articles/com
munique/2010/05.html

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