1. Curriculum vitae
Personal information
Surname de Giorgio
First name Barbara
Date of Birth 6th June 1976
Address 2, via Vallone, 27100, Pavia (PV), Italy
Telephone /Mobile ++39 3480036301
Nationality Italian
Gender Female
Education and
training
Title of qualification awarded PhD Pathology and Genetics
Name and type of organisation
University of Pavia – Laboratory of Molecular Biology (IRCCS Fondazione
providing education and
Policlinico San Matteo)
training
Molecular Biology. Principal arguments of the PhD thesis: analysis and research of
Principal subjects/Occupational
mutations of candidate genes responsible for Marfan Syndrome and gene
skills covered
expression analysis of FBN1.
Dates 2011
Title of qualification awarded Ms Degree in Molecular Biology
Name and type of organisation
University of Milan – Institute of Legal Medicine, via L. Mangiagalli 37, Milano,
providing education and
Italy.
training
Principal subjects/Occupational Forensic Science, study of correlation between the histo-morphological analysis of
skills covered bone tissue and the genetic identification analysis.
Level in national or
109/110
international classification
Dates 2007
Additional Trainings
Title Course of Clinical & Molecular Biology– Rome, 22-26 October 2012 (ECM Course)
Title Biotechnology and Medicine – Milan, 16th November 2011 (ECM Course)
Cytogenetics advanced Course: from conventional to molecular Karyotype – ANM-
Title
FAD (ECM on-line Course) 2011
Hematology – Pavia, IRCCS Policlinico S. Matteo. 10th September 2010 (ECM
Title
Course)
Title Real-Time PCR: 7900HT Basic Training – 4-5 March 2010
Introduction to the Next Generation Sequencing Technology (SOLiD™) – 19th
Title
November 2009
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2. Work Experiencies
Dates 3th September 2012 – Present
Occupation or position held Molecular Biologist
Name and address of employer Laboratory of Molecular Biology (Multimedica Group)
Detection, Quantification and Genotyping of Viral Infection (HCV, HBV, HIV,
Principal subjects/Occupational HPV) and Bacterial Infection (Mycobacterium tuberculosis, Chlamydia
skills covered trachomatis).
Detection of genetic variants in the coagulation factors (FII, FV, MTHFR).
Dates 1th September 2007 – 31th August 2012
Occupation or position held Molecular Biologist
Name and address of employer Laboratory of Molecular Biology (IRCCS Fondazione Policlinico San Matteo)
Analysis and research of mutations of candidate genes responsible for rare genetic
Principal subjects/Occupational disorders (e.g. Breast Cancer, Marfan Syndrome, Noonan Syndrome, Anderson-
skills covered Fabry disease) and Cystic Fibrosis, gene expression analysis, CNV analysis, allelic
discrimination and pharmacogenetics analysis.
Dates 1th November 2005 – 15th May 2007
Occupation or position held Forensic Scientist
Name and address of employer Institute of Legal Medicine, via L. Mangiagalli 37, Milano, Italy
Non Professional
Work Experiencies
Dates 15th May 2007 – 31th August 2007
Occupation or position held Sales Agent for cosmetic products and Trichologist
Name and address of employer Palazzo BenEssere (Integree Group) – Rieti
Dates 05th July 2000 – 31th August 2007
Customer Service Representative (Front office) + boss unit student for GDO at
Occupation or position held CEREF Scrl (Centro Regionale Formazione e Studi Cooperativi v.le Monza 12 -
20127 Milan)
Name and address of employer Auchan S.p.A. - Milan
Dates 01th June 2006 – 31th July 2006
Occupation or position held Call centre operator
Name and address of employer IPSOS Operations Srl - Milan
Dates 01th September 1995 – 31th May 2000
Occupation or position held Management of a pub of family property
Name and address of employer La Taverna del Gaucho - Luky pub - Monza
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3. Teaching Experiences
Dates 28th May 2010 – 30th May 2010
Master Course: “Le Basi Genetiche delle Malattie Cardiovascolari e della Risposta
Title of the training Terapeutica” (The Genetics Basis of Cardiovascolary Disease and the Therapeutic
Response)
Name and address of employer Italian Federation of Cardiology
Dates 01th October 2011 – 03th October 2011
Master Course: “Le Basi Genetiche delle Malattie Cardiovascolari e della Risposta
Title of the training Terapeutica” (The Genetics Basis of Cardiovascolary Disease and the Therapeutic
Response)
Name and address of employer Italian Federation of Cardiology
Name and address of employer University of Pavia – Laboratory of Molecular Biology
Personal skills and
competences
Mother tongue Italian
Other language(s)
Self-assessment Understanding Speaking Writing
European level (*) Listening Reading Spoken Spoken
interaction production
English good good good good good
(*) Common European Framework of Reference (CEF) level
Technical skills and (1) Dna Extraction: from bone, buccal swabs and dry blood with phenol-
competences in Forensic
chloroform or commercial columns;
Science
(2) Amplification of autosomal and sex forensic STR: with forensic PCR
commercial kits (Promega Corporation and Applied Biosystems);
(3) DNA separation and detection: polyacrylammide gel electrophoresis
with silver-staining detection, capillary electrophoresis (ABI Prism 310
Genetic Analyzer) with fluorescence detection;
(4) Sample Interpretation: good knowledge of GenScan® and Genotyper®
software;
(5) Histological Analysis of bone: preparation and analysis of undecalcified
and decalcified sections of bone with three staining methods (EE, PAS,
Feulgen).
Technical skills and (1) DNA and RNA Extraction: from blood, tissues and buccal swabs with
competences
Promega Maxwell®16 System - Personal Automation™ or commercial
columns;
(2) Gene amplification: Primers design with Primer Express®;
(3) Gel electrophoresis: for the analysis of PCR results;
(4) Purifications of PCR products;
(5) Gene sequencing: DNA sequencing with ABI Prism 3130 Genetic Analyzer;
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4. (6) Quantitative PCR: with Applied Biosystems 7900HT Real-Time PCR
System. Analysis of gene expression, genotyping and CNV with ABI
instrument.
Issue and poster (1) de Giorgio B , Favalli V, Grasso M, Serafini E, Cassini P, Canclini C,
Arbustini E. “FBN1 gene expression in Marfan Syndrome: a RT-qPCR pilot
study” q-PCR congress – Dublino (14-15 settembre 2010).
(2) Serafini E, Grasso M, Tarantino P, Canclini C, Guida I, Cassini P, Favalli V, de
Giorgio B, Arbustini E “Clinical study design: influence of CYP450
polymorfisms on drug therapy (Losartan vs Nebivolol vs the association of
both) in genotyped Marfan Syndrome (MFS) patients” q-PCR congress –
Dublino (14-15 settembre 2010).
(3) Favalli V, Magni P, Grasso M, Disabella E , de Giorgio B, Guida I, Tarantino
P, Serafini E, Agozzino M, Arbustini E “Genome-wide gene expression in
aneurysmal aortic walls of patients with Marfan Syndrome (MFS) and
Loeys-Dietz Syndromes (LDS) with known causative mutations” ESC-
Stoccolma (29-31 agosto 2010).
(4) Gambarin F, Massetti M, Serio A, Casotti M, Bozzani A, Dore R, Disabella E,
de Giorgio B, Odero A, Arbustini E. “Dissecazione Aortica: cosa c’ è di
nuovo?” da Conoscere e Curare il Cuore a cura di Francesco Prati, Edizione
Urban 2010, pag.195-215.
(5) M. Diegoli, M. Pasotti, M. Grasso, N. Marziliano, A. Serio, F. Gambarin, P.
Tarantino, A. Pilotto, E. Disabella, P. Cassini, M. Tagliani, B. de Giorgio, E.
Serafini, I. Guida, E. Arbustini “Eventi Predittivi nelle Cardiodistrofinopatie”.
SIGU-TORINO (8-10th November 2009).
(6) E. Disabella, M. Grasso, N. Marziliano, I. Guida, A. Pilotto, M. Diegoli, M.
Tagliani, P. Tarantino, E. Serafini, B. de Giorgio, P. Cassini, E. Arbustini
“Gene ACTA2 e Sindromi da Aneurisma Toracico e Dissecazione Aortica”.
Poster presented at SIGU-TORINO (8-10th November 2009).
(7) P. Cassini, I. Guida, M. Grasso, N. Marziliano, A. Pilotto, E. Serafini, M.
Diegoli, M. Tagliani, E. Disabella, B. de Giorgio, P. Tarantino, E. Arbustini
“Prevalence of Anderson Fabry Disease (AFD) and cardiac involvement”
SIGU-TORINO (8-10th November 2009).
(8) N. Marziliano, M. Grasso, M. Pasotti, M. Tagliani, A. Pilotto, E. Serafini, P.
Cassini, B. de Giorgio, A. Serio, E. Arbustini “Geno-phenotype
characterisation of hypertrophic cardiomyopathy patients that evolve through
end-storage heart failure” European J. Human Genetics (2009) Vol.17
Sup.2:370.
(9) M. Casotti, A. Serio, A. Pilotto, E. Serafini, M. Grasso, N. Marziliano, M.
Tagliani, P. Cassini, B. de Giorgio, M. Agozzino, C. Campana, F. Gambarin,
S. Ghio, M. Vigano’, L. Gavazzi, E. Arbustini “Clinical phenotypes and out
come of 101 LMNA gene mutation carriers”. Circulation (2008) 118: S_955.
(10) Arbustini E, Pilotto A, Grasso M, Marziliano N, Serio A, Gambarin F, Pasotti
M, Serafini E, de Giorgio B. “Novel human pathological mutation. Gene
symbol: LMNA. Disease: Cardiomyopathy, dilated with conduction defects”.
Hum Genet. 2009 Apr; 125 (3):350.
(11) M. Grasso, N. Marziliano, E. Disabella, A. Serio, M. Pasotti, F. Gambarin, A.
Pilotto, E. Serafini, M. Diegoli *, E. Porcu, M. Tagliani, M. Concardi, M.
Agozzino, P. Cassini, B. de Giorgio, P. Tarantino, V. Favalli, I. Guida, A.
Brega ° and E. Arbustini. “TGFBR1 and TGFBR2 gene mutations in Loyes-
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5. Dietz and thoracis aortic aneurysm dissection syndromes”. SIGU-GENOVA
(23-25th November 2008).
(12) M.Diegoli , M. Grasso , N. Marziliano , S. Serio , A. Pilotto , M. Pasotti , E.
Serafini , E. Porcu , P. Cassini , B. de Giorgio , P. Tarantino , I. Guida , F.
Gambarin , A. Brega , C. Monterosso , E. Arbustini. “Bicuspidia Aortica
correlata a mutazioni nel gene NOTCH1”. SIGU-GENOVA (23-25th
November 2008).
(13) M.Grasso , E. Disabella , N.Marziliano , M. Pasotti , A. Pilotto, A. Serio , F.
Gambarin , P. Tarantino , I. Guida , B. de Giorgio , P. Cassini , V. Favalli , A.
Brega , E. Arbustini. “Prevalence and Phenotypes associated with double
Fibrillin-1 gene heterozygosity: clinical relevance for diagnosis, prognosis
and prenatal diagnosis”. SIGU-GENOVA (23-25th November 2008).
(14) N.Marziliano, V. Favalli, M. Grasso, E. Disabella, A. Pilotto, E. Serafini, M.
Diegoli, E. Porcu, M. Tagliani, M. Concardi, M. Agozzino, P. Cassini, B. de
Giorgio, P. Tarantino, I. Guida, M. Massetti, F. Cuttone, A. Brega , E.
Arbustini “Profilatura Genetica mediante microarrays di pazienti con
aneurisma aortico e portatori di mutazione a carico dei geni FBN1, TGFBR1
E TGFBR2”. SIGU-GENOVA (23-25th November 2008).
(15) N Marziliano, A Repetto, E Porcu, A Pilotto, M Tagliani, M Diegoli, B de
Giorgio, M Grasso, M Pasotti, E Arbustini. (116) “Gene expression
signature of adrenaline biosynthesis and inflammatory pathways in women
with left ventricular apical ballooning syndrome” European J. Heart Failure
Supplement (2008) 7, 31.
(16) E.Disabella, E. Serafini, P. Cassini, B. de Giorgio, M. Tagliani, L. Del monte,
N. Marziliano, A. Pilotto, M. Grasso, E. Arbustini “Cornea verticillata in a
female patient revealed the presence of Fabry disease resulting from an in-
frame 12-nucleotide deletion within the GLA gene” 8th International
Symposium on Lysosomal Storage Disorders. Paris, France, April 18-20
(2008).
(17) N Marziliano, M Grasso, A Pilotto, E Serafini, M Tagliani, E Disabella, B
de Giorgio, P Cassini, M Pasotti, E Arbustini “Myocyte function and gene
defects in arrythmogenic right ventricular dysplasia: clinical pnehotypes
and open problems for clinical genetics” European J. Human Genetics (2008)
Vol.16 Sup.2:258.
(18) NMarziliano, M Pasotti, M Grasso, A Pilotto, E Serafini, B de Giorgio, M
Diegoli, A Brega, E Arbustini “Clinical phenotypes and out come of 101
LMNA gene mutation carriers” European J. Human Genetics (2008) Vol.16
Sup.2:24.
(19) M. Pasotti, A. Serio, A. Pilotto, E. Serafini, M. Grasso, N. Marziliano, M.
Tagliani, P. Cassini, B. de Giorgio, M. Agozzino, C. Campana, F.I. Gambarin,
S. Ghio, M. Vigano’, L. Gavazzi, E. Arbustini “Clinical phenotypes and
outcome of 101 LMNA gene mutation carriers” Circulation Suppl. 2008;
118:S_955.
(20)Moda F, Lovisolo A, de Giorgio B, Coco S, Lorenzoni R, Lazzaro A, Piccinini
A. “Protocollo operativo per lo studio della correlazione tra l’analisi isto-
morfologica del tessuto osseo e l’analisi genetico identificativa” (“Operative
protocol for the study of correlation between the histo-morphological
analysis of bone tissue and the genetic identification analysis”), Poster
presented at “Ge.F.I.- 21° National Congress”, Lipari, Italy 5-7 September
2006.
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6. Congress partecipation (1) “Ge.F.I. = Genetisti Forensi Italiani - 21° National Congress”, Lipari, Italy 5-
7th September 2006;
(2) “DNA in Forensics 2006”, Innsbruck, Austria 26-30th September 2006
(3) “Vivere con la Sindrome di Marfan” Pavia, Italy 22th Settember 2007;
(4) “Vivere con una Cardiomiopatia” Pavia, Italy 27th October 2007;
(5) “Question and Answers – Workshop on Inherited Cardiomyopaties” Camogli,
Italy 4-5th April 2008;
(6) “Vivere con una Cardiomiopatia” Pavia, Italy 27th Settember 2008;
(7) “La Sindrome di Marfan e le Sindromi Correlate: Lotta alla Dissecazione
Aortica” Pavia, Italy 04th October 2008;
(8) 9th Meeting on : “Clinical Application of Real Time PCR Technology”
Florence, Italy 26th Settember 2008;
(9) 1° Congresso GIMAF: “Screening di Popolazione a Rischio per Malattia di
Fabry: esperienze a confronto” Pavia, Italy 28 Marzo 2009;
(10) “Question and Answers – Workshop on Inherited Cardiomyopaties” Camogli,
Italy 3-4th April 2009.
(11) “XII Congresso Nazionale SIGU” (Italian Society of Human Genetics) Turin,
Italy 8-10th November 2009.
(12) “q-PCR” Dublin, Ireland 14-15 september 2010.
Computer skills and Good knowledge of Windows, Word, Excel, Power Point, Internet Explorer,
competences Mozilla Firefox, Genescan and Genotyper software.
Other skills and Good ability in bibliographic retrieval with the chief search engines (Pubmed,
competences NCBI, Ensembl, Scirus, Reference Manager and Google Scholar)
Driving licence(s) European Driving Licence B obtained in 1995
Referees  MD Fabiana Gambarin, Centro Malattie Genetiche Cardiovascolari -
Laboratory of Molecular Biology (IRCCS Fondazione Policlinico San
Matteo) P.le Golgi n. 19, Pavia, Italy.
Mail: f_gambarin@yahoo.it
 PhD Federico Sebastiani, Dako, Sales & Support Manager Italy, via Piero
Portaluppi 11/2, Milano, Italy.
Mail: federico.sebastiani@hotmail.it
 MD, PhD Andrea Piccinini, Institute of Legal Medicine, University of Milan,
Forensic Genetics Laboratory, via L. Mangiagalli 37, Milano, Italy.
Phone: ++39(0)2.50315706
Mail: andrea.piccinini@unimi.it
DL 196/03 Il sottoscritto/a, acquisita l’informativa di cui all’art. 13 Decreto Legislativo 196/03
con l’invio del proprio CV presta il proprio consenso, ai sensi degli art. 23 e 26 del
decreto stesso, al trattamento dei dati personali, anche sensibili, che ha ritenuto
opportuno indicare nel CV.
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